mRNA vaccine activation of dendritic cell and induction of IFN-1 (WP5187)

Homo sapiens

After uptake, mRNA is translated into spike protein and presented as cell-surface MHC-bound peptides to CD4+ and CD8+ T cells. Cytosolic sensing of RNA by RIG-I and MDA5 plus TLR binding within endosomes leads to activation of IFN regulatory factor 3/7 (IRF3/7) and nuclear factor κB (NF-κB), which bind to DNA inducing gene transcription, and production of IFN-I and proinflammatory cytokines, respectively


Nhung Pham , Eric Weitz , Egon Willighagen , and Alex Pico


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Homo sapiens




Pathway Ontology

disease pathway

Disease Ontology


Cell Type Ontology

dendritic cell CD8-positive, alpha-beta T cell CD4-positive, alpha-beta T cell


Label Type Compact URI Comment
Spike Protein Protein uniprot:P0DTC2
S peptide presentation Protein uniprot:Q95460
MAVS Protein uniprot:Q7Z434
IRF-7 Protein uniprot:Q92985
IRF3 Protein uniprot:Q14653
RIG-I (DDX58) Protein uniprot:O95786
MDA5 Protein uniprot:Q9BYX4
T cell receptor Protein uniprot:P01848
TLR3 Protein uniprot:O15455
NF-kB Protein uniprot:Q9Y6Q6
TLR7 Protein uniprot:Q9NYK1
TLR8 Protein uniprot:Q9NR97
S peptide presentation Protein uniprot:Q95460
T cell receptor Protein uniprot:P01848


  1. COVID-19 vaccine side effects: The positives about feeling bad. Sprent J, King C. Sci Immunol. 2021 Jun 22;6(60):eabj9256. PubMed Europe PMC Scholia