ArcPathVisio
Brace
Octagon
Ellipse
EndoplasmicReticulum
HexagonPathVisio
GolgiApparatus
MimDegradation
Mitochondria
Octagon
PentagonPathVisio
Rectangle
RoundedRectangle
SarcoplasmicReticulum
TrianglePathVisio
none
5' G cap
pre-mRNA
hnRNPs
3'
iPo
Primary RNA transcript
3'
Rngtt (capping enzyme)
Rnmt (methyltransferase)
5'
5'
3'
5'
Polr2a
3'
5'
5'
Misc.
U5 snRNP
RNA helicase
Additional Factors
YYYY
mRNA Capping
3'
Processing of Intron-Containing Pre-mRNA
Cap Binding
65
Formation of the Spliceosomal E complex
exon 1
Cleavage of mRNA at the 3'-end
P-
A
A
U6 snRNP
RNA helicase A
SR-binding sites
mRNA 3'-end processing
Pi
Accessory Proteins
Additional SR proteins
AAUAAA
Poly(A) pol
Internal methylation of mRNA
RNAi pathway
exon 1
mRNA splicing (major pathway)
hnRNP regulators
CFI
exon 2
snRNP Sm core complex
UG
3'
U4:U5:U6 tri-snRNP complex
exon 2
(2c)
UG
Additional Factors
SR protein kinases
U4 snRNP
splicing factor SF3A
Additional Factors
AG
Kinases Phosphorylating RS domains?
Formation of the Spliceosomal B complex
Formation of pre-mRNPs
SR-binding sites
Formation of the Spliceosomal A complex
splicesome associated helicases
CPSF
5'
3'
SR-repressor proteins
Serine/Argenine Rich Proteins(SR)
Lariat Formation and 5'-Splice Site Cleavage
YYYY
Formation of the active Spliceosomal C complex
U4/U6
35
(2a)
right click on blue text for detailed information
exon 1
5'
A
A
P
5'
mRNA polyadenylation
exon 1
3'
CF I
splicing factor SF3B
Formation of the Spliceosomal B complex
Putative Alternative Splicing
Regulators
Methylation of mRNA
Formation of the Spliceosomal A complex
U5
YYYY
U6
Formation of the Spliceosomal E complex
U2
exon 1
SR protein phosphatases
YYYY
degraded
AG
heterogeneous nuclear ribonucleoproteins
exon 1
U2AF
CPSF
AG
exon 2
Poly(A) polymerase alpha
mRNA splice site selection
A
Regulation of
Alternative Splicing
YYYY
5'
U2AF
U4
Note: New participating members shown at each step
SR-proteins
U1 snRNP
CA
AG
SF3b14b
exon 2
(1)
SR-proteins
U2 snRNP
AG
CstF
YYYY
U1
CstF
U1 snRNP
Spt5
mRNA capping
AG
snRNP Sm core complex
CF II
SR-kinases
U2 snRNPs
3'
Formation of the Spliceosomal C complex
(2b)
exon 2
Formation of pre-mRNPs
Internal methylation of mRNA
exon 2
(3)
intron
(2)
CF II
snRNP Sm core complex
AAAAAAA
Poly(A)pb II
SR-proteins
5'
U5
A
5'
GU
Poly(A)-binding protein II
3'
U2 snRNP
Cleavage at the 3'-Splice-Site and Exon Ligation
SR-phosphatases
3'
RBM17
DNAJC8
XRN2
DHX16
CELF2
SPOP
PHF5A
NSEP1
SUGP1
PRPF40A
PSKH1
PCBP2
LSM7
DDX1
SRPK1
TRA2B
SRPK2
FAM177A1
PRPF4B
CELF1
CLP1
METTL3
NXF1
SFPQ
RBM5
PRPF3
SRSF10
PAPOLA
CLASRP
DICER1
PABPN1
U2
PRMT1
SFSWAP
CD2BP2
SRSF10
TMED10
PPM1G
NUDT21
DHX38
SREK1
SRSF4
CLK2
PRPF8
GMP
SNRPN
CLK4
PRMT2
DDX20
NONO
CELF4
PRPF18
PTBP1
SNRPN
ATP
DHX9
SUGP2
SRRM1
CDC40
SMC1A
CDC40
DHX8
CLK1
CLK3
PTBP2
RBM39
FUS
RNPS1
DHX15
SSFA1
LSM2
PRPF8
EFTUD2
U2AF1
U2AF2
Rnu6
SSFA1
PRPF4
LSM2
PRPF4
U4 snRNA
SSFA1
SF3A2
SF3A1
SF3A3
SF3B5
SF3B3
SF3B2
SF3B4
SF3B1
SRSF9
SRSF1
SRSF6
SFRS3
SRSF2
SRSF5
SRSF7
SNRPA1
SNRPB2
RNGTT
SUPT5H
POLR2A
RNMT
SNRPD3
SNRPD1
SNRPD2
SNRPF
SNRPE
SNRPG
SNRPB
CSTF3
CSTF2
CSTF2T
CSTF1
HNRNPM
HNRNPA1
HNRNPA2B1
HNRNPR
HNRPH2
HNRNPU
HNRPA3P1
HNRNPK
HNRNPD
HNRNPAB
HNRNPC
HNRNPL
RBMX
HNRNPH2
NCBP2
NCBP1
CPSF3
CPSF4
CPSF2
CPSF1
U1 snRNA
SNRNP70
SNRPA
PRPF6
SNRNP40
U5 snRNA
TXNL4A
Name: mRNA Processing
Last Modified: 20180119142026
Organism: Pan troglodytes