Eukaryotic transcription initiation (WP830)

Gallus gallus

In eukaryotes, RNA polymerase, and therefore the initiation of transcription, requires the presence of a core promoter sequence in the DNA. RNA polymerase is able to bind to core promoters in the presence of various specific transcription factors. The most common type of core promoter in eukaryotes is a short DNA sequence known as a TATA box. The TATA box, as a core promoter, is the binding site for a transcription factor known as TATA binding protein (TBP), which is itself a subunit of another transcription factor, called Transcription Factor II D (TFIID). After TFIID binds to the TATA box via the TBP, five more transcription factors and RNA polymerase combine around the TATA box in a series of stages to form a preinitiation complex. One transcription factor, DNA helicase, has helicase activity and so is involved in the separating of opposing strands of double-stranded DNA to provide access to a single-stranded DNA template. However, only a low, or basal, rate of transcription is driven by the preinitiation complex alone. Other proteins known as activators and repressors, along with any associated coactivators or corepressors, are responsible for modulating transcription rate. Source: [[wikipedia:Transcription_(genetics)|Wikipedia]]


Thomas Kelder , Kristina Hanspers , Christine Chichester , and Eric Weitz


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Gallus gallus



Pathway Ontology

RNA polymerase II transcription initiation pathway RNA polymerase I transcription initiation pathway RNA polymerase III transcription initiation pathway


Label Type Compact URI Comment
TBP GeneProduct ncbigene:395995
GTF2B GeneProduct ncbigene:424518
RCJMB04_15p4 GeneProduct ncbigene:423872
TAF6 GeneProduct ncbigene:776310
TAF7 GeneProduct ncbigene:422192
TAF9 GeneProduct ncbigene:422152
ILK GeneProduct ncbigene:374018
TAF12 GeneProduct ncbigene:419585
TAF13 GeneProduct ncbigene:418365
GTF2E1 GeneProduct ncbigene:418328
GTF2A2 GeneProduct ncbigene:770910
GTF2F2 GeneProduct ncbigene:418843
GTF2E2 GeneProduct ncbigene:771227
CDK7 GeneProduct ncbigene:771294
GTF2H1 GeneProduct ncbigene:423081
ERCC3 GeneProduct ncbigene:424226
POLR1B GeneProduct ncbigene:416710
POLR3B GeneProduct ncbigene:418071
POLR2F GeneProduct ncbigene:395486
POLR2C GeneProduct ncbigene:415635
POLR2E GeneProduct ncbigene:420103
POLR2K GeneProduct ncbigene:768387
POLR3E GeneProduct ncbigene:416620
POLR3H GeneProduct ncbigene:417990
POLR3K GeneProduct ncbigene:768505
POLR1A GeneProduct ncbigene:422910
POLR2I GeneProduct ncbigene:426289
POLR2H GeneProduct ncbigene:424954
POLR2B GeneProduct ncbigene:422621
CCNH GeneProduct ncbigene:427328
MNAT1 GeneProduct ncbigene:423521
GTF2H3 GeneProduct ncbigene:771837
POLR1E GeneProduct ensembl:ENSGALG00000005395
POLR1D GeneProduct ncbigene:428713


  1. Regulation of gene expression by TBP-associated proteins. Lee TI, Young RA. Genes Dev. 1998 May 15;12(10):1398–408. PubMed Europe PMC Scholia
  2. Orchestrated response: a symphony of transcription factors for gene control. Lemon B, Tjian R. Genes Dev. 2000 Oct 15;14(20):2551–69. PubMed Europe PMC Scholia