Trehalose degradation, low osmolarity (WP70)

Saccharomyces cerevisiae

Trehalose is a storage carbohydrate that can either be synthesized or obtained from the external environment. To be utilized as a carbon source, trehalase (EC: must convert trehalose and water into two molecules of glucose. S. cerevisiae has two trehalase enzymes, one is an acid trehalase encoded by ATH1 and the other is a neutral trehalase encoded by NTH1. The name "acid" or "neutral" are based on the optimal pH of each enzyme (pH 4.5-5.0 and pH 6.8-7.0, respectively). Each trehalase enzyme is active in a different subcellular location. Nth1p occurs as homodimer that is located in the cytoplasm, and is required for the hydrolysis of intracellular trehalose. Intracellular trehalose either results from trehalose biosynthesis, or to a lesser degree from uptake of trehalose from the environment via the Mal11p transporter. Ath1p was originally predicted to be a vacuolar protein, but has been experimentally shown to mainly localize in the periplasmic space, with a small fraction also occurring in the cell wall. Extracellular trehalose is hydrolysed into 2 glucose molecules by Ath1p. Source:


Meredith Braymer , Egon Willighagen , Daniela Digles , Denise Slenter , Finterly Hu , and Eric Weitz


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Saccharomyces cerevisiae



Pathway Ontology

trehalose degradation pathway


Label Type Compact URI Comment
H2O Metabolite chebi:15377
beta-D-Glucose Metabolite chebi:15903
alpha,alpha-trehalose 6-phosphate Metabolite chebi:58429
H2O Metabolite chebi:15377
H2O Metabolite chebi:15377
glucose-6-phosphate Metabolite chebi:75052
ATP Metabolite cas:1927-31-7
ADP Metabolite cas:58-64-0
phosphate Metabolite cas:14265-44-2
glucose-6-phosphate Metabolite chebi:75150
trehalose Metabolite cas:99-20-7
ATH1 GeneProduct sgd:S000006230 For conversion of acid trehalose
GLK1 GeneProduct sgd:S000000545
NTH2 GeneProduct sgd:S000000205
TPS2 GeneProduct sgd:S000002481
NTH1 GeneProduct sgd:S000002408 For c onversion of neutral trehalose