Metabolic pathways of fibroblasts (WP5312)

Homo sapiens

Fibroblasts are known to contribute to tissue and organ connectivity by producing and secreting extracellular matrix (ECM) proteins. Although several diseases, such as fibrosis and cancer, have been linked to changes in fibroblast metabolism, the resting metabolic conditions that are present in healthy cells generally go unreported. Because fibroblasts are known to display heterogeneity, the metabolic processes of different tissues may vary. The aim was to identify and map out the general metabolic pathways present in fibroblasts. The pathways for collagen, serine, and proline, as well as glycosaminoglycan synthesis, are nearly complete. Nonetheless, dashed arrows indicate missing process steps that should be identified and included. There is a lack of data on fibroblasts' metabolic activities, and further research is required to understand the complete metabolic pathways of fibroblasts.


AliHbdl , Jgumbaragyte , Ecenazertan , Eric Weitz , Mariamierzejewska , Alippifed , MatejKrivulka , Egon Willighagen , Andra Waagmeester , and Alex Pico


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Homo sapiens



Pathway Ontology

classic metabolic pathway

Disease Ontology

pulmonary fibrosis

Cell Type Ontology



Label Type Compact URI Comment
Heparan sulfate Metabolite hmdb:HMDB00693
Hyaluronic acid Metabolite hmdb:HMDB02061
Chondroitin sulfate Metabolite chebi:37397
Keratan sulfate Metabolite chebi:60924
UDP-GlcA Metabolite chebi:17200
Fatty acid Metabolite chebi:35366
Dermatan sulfate Metabolite hmdb:HMDB00632
Glycine Metabolite chebi:15428
Proline Metabolite chebi:17203
P5C Metabolite chebi:15893
Collagen Metabolite chebi:3815
Serine Metabolite chebi:17822
Glutamate Metabolite chebi:29985
G6P Metabolite hmdb:HMDB01401
Acetyl-CoA Metabolite hmdb:HMDB01206
Pyruvate Metabolite hmdb:HMDB00243
Glutamine Metabolite chebi:18050
αKG Metabolite hmdb:HMDB00208
Glucose Metabolite hmdb:HMDB00122
3PG Metabolite hmdb:HMDB00807
F6P Metabolite chebi:15946
Lactic acid Metabolite hmdb:HMDB00190
Glutamine Metabolite chebi:18050
L-glutamate Metabolite chebi:29985
Glutamine Metabolite chebi:18050
Glucose Metabolite hmdb:HMDB00122
Lactic acid Metabolite hmdb:HMDB00190
G1P Metabolite chebi:29042
UDP-glucose Metabolite chebi:18066
GSAL Metabolite wikidata:Q2823261
Fatty acid Metabolite chebi:35366
FGFR1 GeneProduct uniprot:P11362 Full name: Fibroblast growth factor receptor 1 Gene: FGFR1
CD36 GeneProduct uniprot:P16671 Full name: Platelet glycoprotein 4 Gene: CD36
G6PI GeneProduct uniprot:P06744 Full name: Glucose-6-phosphate isomerase Gene: GPI
P3H4 GeneProduct uniprot:Q92791 Full name: Endoplasmic reticulum protein SC65 Gene: P3H4
LPAR1 GeneProduct ensembl:ENSG00000198121
LOX GeneProduct uniprot:Q9Y4K0 Full name: Lysyl oxidase homolog 2 Gene: LOXL2
BMP1 GeneProduct uniprot:P13497 Full name: Bone morphogenetic protein 1 Gene: BMP1
FGFR4 GeneProduct uniprot:P22455 Full name: Fibroblast growth factor receptor 4 Gene: FGFR4
ADAMTS2 GeneProduct uniprot:O95450 Full name: A disintegrin and metalloproteinase with thrombospondin motifs 2 Gene: ADAMTS2
P3H3 GeneProduct uniprot:Q8IVL6 Full name: Prolyl 3-hydroxylase 3 Gene: P3H3
Hsp47 GeneProduct uniprot:P50454 Full name: Serpin H1 Gene: SERPINH1
LDH GeneProduct uniprot:P00338 Full name: L-lactate dehydrogenase A chain Gene: LDHA
SLC1A5 GeneProduct uniprot:Q15758 Full name: Neutral amino acid transporter B(0) Gene: SLC1A5
PLOD1 GeneProduct uniprot:Q02809 Full name: Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 Gene: PLOD1
P4HA3 GeneProduct uniprot:Q7Z4N8 Full name: Prolyl 4-hydroxylase subunit alpha-3 Gene: P4HA3
P4HA1 GeneProduct uniprot:P13674 Full name: Prolyl 4-hydroxylase subunit alpha-1 Gene: P4HA1
P4HA2 GeneProduct uniprot:O15460 Full name: Prolyl 4-hydroxylase subunit alpha-2 Gene: P4HA2
SLC1A5 GeneProduct uniprot:Q15758 Full name: Neutral amino acid transporter B(0) Gene: SLC1A5
UGDH Protein uniprot:O60701
P5CR Protein uniprot:P32322 Full name: Pyrroline-5-carboxylate reductase 1, mitochondrial Gene: PYCR1
GLUT1 Protein uniprot:P11166 Full name: Solute carrier family 2, facilitated glucose transporter member 1 Gene: SLC2A1
GLS Protein uniprot:O94925 Full name: Glutaminase kidney isoform, mitochondrial Gene: GLS
GLDH Protein uniprot:P00367 Full name: Glutamate dehydrogenase 1 Gene: GLUD1
SHMT Protein uniprot:O62585 Full name: Serine hydroxymethyltransferase, cytosolic Gene: SHMT1
UGP2 Protein uniprot:Q16851 Full name: UTP--glucose-1-phosphate uridylyltransferase Gene: UGP2
PHGDH Protein uniprot:O43175 Full name: D-3-phosphoglycerate dehydrogenase Gene: PHGDH
RhoA Protein uniprot:P61586 Full name: Transforming protein RhoA/Ras homolog family member A Gene: RHOA
P5CS Protein uniprot:P54886 Full name: Delta-1-pyrroline-5-carboxylate synthase Gene: ALDH18A1
PSP Protein uniprot:P78330 Full name: Phosphoserine phosphatase Gene: PSPH
PLC Protein uniprot:P19174 Full name: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 Gene: PLCG1
PSA Protein uniprot:Q9Y617 Full name: Phosphoserine aminotransferase Gene: PSAT1
HK Protein uniprot:P35557 Full name: Hexokinase-4 Gene: GCK
GLS Protein uniprot:O94925 Full name: Glutaminase kidney isoform, mitochondrial Gene: GLS
MCT1 Protein uniprot:P53985 Full name: Monocarboxylate transporter 1 Gene: SLC16A1
PGM1 Protein uniprot:P36871 Full name: Phosphoglucomutase-1 Gene: PGM1
BMP1 Protein uniprot:P13497 Full name: Bone morphogenetic protein 1 Gene: BMP1


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