Acetylcholine synthesis (WP528)

Homo sapiens

Acetylcholine is an important neurotransmitter. It can be rapidly released in the synaptic cleft upon activation of the neuron. In the synaptic cleft the compound is degraded rapidly into choline and acetate, this is essential for proper neuronal functioning. Choline and Acetate are taken up into the cytosol and recycled for the next activation.


Andrew Kwa , Kristina Hanspers , Hubert Hug , Martijn Van Iersel , Thomas Kelder , Egon Willighagen , Anders Riutta , Anwesha Bohler , Martina Summer-Kutmon , and Eric Weitz


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Homo sapiens



Cell Type Ontology

cholinergic neuron

Pathway Ontology

acetylcholine metabolic pathway


Label Type Compact URI Comment
Cytidine diphosphate choline Metabolite hmdb:HMDB0001413
Phosphatidylethanolamine Metabolite hmdb:HMDB0060501
Phosphorylcholine Metabolite hmdb:HMDB0001565
Acetylcholine Metabolite cas:51-84-3
Choline Metabolite cas:62-49-7
Glycerophosphocholine Metabolite cas:28319-77-9
Phosphatidylcholine Metabolite hmdb:HMDB0000564
Pyruvate Metabolite hmdb:HMDB0000243
Acetyl CoA Metabolite cas:72-89-9
Acetate Metabolite cas:64-19-7
Choline Metabolite cas:62-49-7
PCYT1A GeneProduct ncbigene:5130
PDHA2 GeneProduct ncbigene:5161
ACHE GeneProduct ncbigene:43
PDHA1 GeneProduct ncbigene:5160
PEMT GeneProduct ncbigene:10400
CHKA GeneProduct ncbigene:1119
CHAT GeneProduct ncbigene:1103


  1. Choline and cholinergic neurons. Blusztajn JK, Wurtman RJ. Science. 1983 Aug 12;221(4611):614–20. PubMed Europe PMC Scholia