N-glycan biosynthesis (WP5153)

Homo sapiens

This pathways describes the biosynthesis of N-linked (Asparagine-linked) glycans, to form glycoproteins. This process is a type of co-translational and post-translational modification and sometimes referred to as N-linked glycosylation. Glycosylation is important for many processes, including protein folding and cell-cell adhesion and immune function. This pathway was modeled based on Figure 9.3 and 9.4 from [https://www.ncbi.nlm.nih.gov/books/NBK453020/ Essentials of Glycobiology [Internet]. 3rd edition], Figure 17.1 from [https://link.springer.com/chapter/10.1007/978-981-13-2835-0_17 Dall'Olio] and [https://www.genome.jp/pathway/hsa00510 KEGG].


Kristina Hanspers and Egon Willighagen


last edited

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Homo sapiens



Pathway Ontology

N-linked glycan biosynthetic pathway protein modification pathway


Label Type Compact URI Comment
Dolichol Metabolite chebi:88766
G10595 Metabolite kegg.glycan:G10595
Polyprenol Metabolite chebi:26199
dolichyl diphosphate Metabolite hmdb:HMDB01513
Dolichyl phosphate D-mannose Metabolite hmdb:HMDB00994
Fructose-6-phosphate Metabolite hmdb:HMDB0000124
GDP-D-mannose Metabolite chebi:84880
G00171 Metabolite kegg.glycan:G00171
G00010 Metabolite kegg.glycan:G00010
G00011 Metabolite kegg.glycan:G00011
G10694 Metabolite kegg.glycan:G10694
G00012 Metabolite kegg.glycan:G00012
G00013 Metabolite kegg.glycan:G00013
G00014 Metabolite kegg.glycan:G00014
G00015 Metabolite kegg.glycan:G00015
G00019 Metabolite kegg.glycan:G00019
G00020 Metabolite kegg.glycan:G00020
G00016 Metabolite kegg.glycan:G00016
G00021 Metabolite kegg.glycan:G00021
G00022 Metabolite kegg.glycan:G00022
G00017 Metabolite kegg.glycan:G00017
G00018 Metabolite kegg.glycan:G00018
G10596 Metabolite kegg.glycan:G10596
G10597 Metabolite kegg.glycan:G10597
G00007 Metabolite kegg.glycan:G00007
G10598 Metabolite kegg.glycan:G10598
G10599 Metabolite kegg.glycan:G10599
G00008 Metabolite kegg.glycan:G00008
G00006 Metabolite kegg.glycan:G00006
G00009 Metabolite kegg.glycan:G00009
dolichyl phosphate Metabolite chebi:16214
G00001 Metabolite kegg.glycan:G00001
G00002 Metabolite kegg.glycan:G00002
G00003 Metabolite kegg.glycan:G00003
G00004 Metabolite kegg.glycan:G00004
G00005 Metabolite kegg.glycan:G00005
G10526 Metabolite kegg.glycan:G10526
G00006 Metabolite kegg.glycan:G00006
dolichyl β-D-glucosyl phosphate Metabolite chebi:15812
dolichyl phosphate Metabolite chebi:16214
dolichyl phosphate Metabolite chebi:16214
Glucose 6-phosphate Metabolite hmdb:HMDB0001401
Mannose-6-phosphate Metabolite hmdb:HMDB0001078
D-mannose 1-phosphate Metabolite chebi:35374
G10694 Metabolite kegg.glycan:G10694
MOGS GeneProduct ensembl:ENSG00000115275
MPDU1 GeneProduct ensembl:ENSG00000129255
DAD1 GeneProduct ensembl:ENSG00000129562
STT3B GeneProduct ensembl:ENSG00000163527
DOLPP1 GeneProduct ensembl:ENSG00000167130
RFT1 GeneProduct ensembl:ENSG00000163933
ALG10 GeneProduct ensembl:ENSG00000139133
GANAB GeneProduct ensembl:ENSG00000089597
MAN1B1 GeneProduct ensembl:ENSG00000177239
MGAT1 GeneProduct ensembl:ENSG00000131446
MAN1A1 GeneProduct ensembl:ENSG00000111885
MAN2A1 GeneProduct ensembl:ENSG00000112893
MAN1A2 GeneProduct ensembl:ENSG00000198162
MAN1C1 GeneProduct ensembl:ENSG00000117643
MAN2A2 GeneProduct ensembl:ENSG00000196547
MGAT2 GeneProduct ensembl:ENSG00000168282
MGAT3 GeneProduct ensembl:ENSG00000128268
FUT8 GeneProduct ensembl:ENSG00000033170
B4GALT1 GeneProduct ensembl:ENSG00000086062
B4GALT2 GeneProduct ensembl:ENSG00000117411
B4GALT3 GeneProduct ensembl:ENSG00000158850
ST6GAL1 GeneProduct ensembl:ENSG00000073849
ST6GAL2 GeneProduct ensembl:ENSG00000144057
MGAT5 GeneProduct ensembl:ENSG00000152127
MGAT4C GeneProduct ensembl:ENSG00000182050
ALG8 GeneProduct ensembl:ENSG00000159063
ALG6 GeneProduct ensembl:ENSG00000088035
ALG9 GeneProduct ensembl:ENSG00000086848
ALG12 GeneProduct ensembl:ENSG00000182858
ALG3 GeneProduct ensembl:ENSG00000214160
STT3A GeneProduct ensembl:ENSG00000134910
DDOST GeneProduct ensembl:ENSG00000244038
RPN1 GeneProduct ensembl:ENSG00000163902
RPN2 GeneProduct ensembl:ENSG00000118705
TUSC3 GeneProduct ensembl:ENSG00000104723
DOLK GeneProduct ensembl:ENSG00000175283
SRD5A3 GeneProduct ensembl:ENSG00000128039
DPAGT1 GeneProduct ensembl:ENSG00000172269
ALG13 GeneProduct ensembl:ENSG00000101901
ALG14 GeneProduct ensembl:ENSG00000172339
ALG1 GeneProduct ensembl:ENSG00000033011
ALG2 GeneProduct ensembl:ENSG00000119523
ALG11 GeneProduct ensembl:ENSG00000253710
DPM1 GeneProduct ensembl:ENSG00000000419
DPM2 GeneProduct ensembl:ENSG00000136908
DPM3 GeneProduct ensembl:ENSG00000179085
MPDU1 GeneProduct ensembl:ENSG00000129255
ALG5 GeneProduct ensembl:ENSG00000120697
GMPPA GeneProduct ensembl:ENSG00000144591
GMPPB GeneProduct ensembl:ENSG00000173540
PMM2 GeneProduct ensembl:ENSG00000140650
MPI GeneProduct ensembl:ENSG00000178802
GPI GeneProduct ensembl:ENSG00000105220
MGAT4B GeneProduct hgnc.symbol:MGAT4B
MGAT4A GeneProduct ncbigene:11320
MGAT4D GeneProduct ncbigene:152586
MGAT5B GeneProduct ncbigene:146664
ALG10B GeneProduct ensembl:ENSG00000175548


  1. KEGG Pathway: hsa00510
  2. A mutation in the human MPDU1 gene causes congenital disorder of glycosylation type If (CDG-If). Kranz C, Denecke J, Lehrman MA, Ray S, Kienz P, Kreissel G, et al. J Clin Invest. 2001 Dec;108(11):1613–9. PubMed Europe PMC Scholia
  3. N-Glycans. Stanley P, Taniguchi N, Aebi M. In: Varki A, Cummings RD, Esko JD, Stanley P, Hart GW, Aebi M, et al., editors. Essentials of Glycobiology. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2017. p. 99–111. PubMed Europe PMC Scholia
  4. Glycobiology of Aging. Dall’Olio F. Subcell Biochem. 2018;90:505–26. PubMed Europe PMC Scholia