One-carbon metabolism (WP435)

Mus musculus

This one-carbon metabolism pathway is centered around folate. Folate is the primary methyl-group donor for processes such as DNA methylation reactions, nucleotide synthesis and DNA repair mechanisms. An important pathway for any study related to folate or DNA methylation. Differential methylation (e.g. hypermethylation of tumor suppressors) as well as disturbances in nucleotide synthesis and repair, are associated with several forms of cancer. There are also indications that hypermethylation is involved in the progression of adenomas to cancer. Inferred from human one-carbon metabolism pathway.


Michiel Adriaens , Kristina Hanspers , Alex Pico , Chris Evelo , Daniela Digles , Martina Summer-Kutmon , Egon Willighagen , Friederike Ehrhart , and Eric Weitz


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Mus musculus




Pathway Ontology

folate mediated one-carbon metabolic pathway


Label Type Compact URI Comment
Tetrahydrofolate Metabolite cas:135-16-0
L-Methionine Metabolite hmdb:HMDB0000696
5-Formiminotetrahydrofolic acid Metabolite hmdb:HMDB0001534
5-Formyltetrahydrofolate Metabolite hmdb:HMDB0001562
5,10-Methenyl Tetrahydrofolate Metabolite hmdb:HMDB0001354
Dihydrofolate Metabolite cas:4033-27-6
Monoglutamate Metabolite cas:59-30-3
Glycine Metabolite hmdb:HMDB0000123
S-Adenosylhomocysteine Metabolite hmdb:HMDB0000939
Polyglutamate Metabolite cas:25513-46-6
5-Methyl Tetrahydrofolate Metabolite cas:134-35-0
Riboflavin/Vitamin B2 Metabolite hmdb:HMDB0000244
Homocysteine Metabolite hmdb:HMDB0000742
dUMP Metabolite chebi:17622
Methylcobalamin Metabolite hmdb:HMDB0002274
L-Serine Metabolite hmdb:HMDB0000187
dTMP Metabolite chebi:17013
Ethanol Metabolite hmdb:HMDB0000108
S-Adenosylmethionine Metabolite chebi:15414
Cobalamin Metabolite hmdb:HMDB0002174
10-Formyl Tetrahydrofolate Metabolite cas:2800-34-2
Betaine Metabolite hmdb:HMDB0000043
5,10-Methylene Tetrahydrofolate Metabolite chebi:12071
Mthfd1 GeneProduct ncbigene:108156
Mthfd1 GeneProduct ncbigene:108156
Mtfmt GeneProduct ncbigene:69606
Dnmt1 GeneProduct ncbigene:13433
Gart GeneProduct ncbigene:14450
Dnmt3b GeneProduct ncbigene:13436
Dhfr GeneProduct ncbigene:13361
Matia GeneProduct ncbigene:11720
Bhmt GeneProduct ncbigene:12116
Mthfd1 GeneProduct ncbigene:108156
Mat2b GeneProduct ncbigene:108645
Atic GeneProduct ncbigene:108147
Ftcd GeneProduct ncbigene:14317
Tcn II GeneProduct ncbigene:21452
Mthfd2 GeneProduct ncbigene:17768
Amt GeneProduct ncbigene:434437
Shmt2 GeneProduct ncbigene:108037
Mtr GeneProduct ncbigene:238505
Amt GeneProduct ncbigene:434437
Folh1 GeneProduct ncbigene:53320
Ehmt1 GeneProduct ncbigene:77683
Ahcy GeneProduct ncbigene:269378
Shmt1 GeneProduct ncbigene:20425
Mthfd1l GeneProduct ncbigene:270685
Mthfd2 GeneProduct ncbigene:17768
Dnmt3a GeneProduct ncbigene:13435
Mthfr GeneProduct ncbigene:17769
Mtrr GeneProduct ncbigene:210009
Ehmt2 GeneProduct ncbigene:110147
Mthfd1l GeneProduct ncbigene:270685
Ftcd GeneProduct ncbigene:14317
Mthfd1l GeneProduct ncbigene:270685
Aldh1l1 GeneProduct ncbigene:107747
Kiaa0828 GeneProduct ncbigene:74340
Gart GeneProduct ncbigene:14450
Dhfr GeneProduct ncbigene:13361
Tyms GeneProduct ncbigene:22171
Dhfr GeneProduct ncbigene:13361
Mthfs GeneProduct ncbigene:107885


  1. Twenty-four non-synonymous polymorphisms in the one-carbon metabolic pathway and risk of colorectal adenoma in the Nurses’ Health Study. Hazra A, Wu K, Kraft P, Fuchs CS, Giovannucci EL, Hunter DJ. Carcinogenesis. 2007 Jul;28(7):1510–9. PubMed Europe PMC Scholia