Eukaryotic transcription initiation (WP425)

Saccharomyces cerevisiae

In eukaryotes, RNA polymerase, and therefore the initiation of transcription, requires the presence of a core promoter sequence in the DNA. RNA polymerase is able to bind to core promoters in the presence of various specific transcription factors. The most common type of core promoter in eukaryotes is a short DNA sequence known as a TATA box. The TATA box, as a core promoter, is the binding site for a transcription factor known as TATA binding protein (TBP), which is itself a subunit of another transcription factor, called Transcription Factor II D (TFIID). After TFIID binds to the TATA box via the TBP, five more transcription factors and RNA polymerase combine around the TATA box in a series of stages to form a preinitiation complex. One transcription factor, DNA helicase, has helicase activity and so is involved in the separating of opposing strands of double-stranded DNA to provide access to a single-stranded DNA template. However, only a low, or basal, rate of transcription is driven by the preinitiation complex alone. Other proteins known as activators and repressors, along with any associated coactivators or corepressors, are responsible for modulating transcription rate. Source: [[wikipedia:Transcription_(genetics)|Wikipedia]]


Nathan Salomonis , Thomas Kelder , Christine Chichester , and Eric Weitz


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Saccharomyces cerevisiae



Pathway Ontology

transcription pathway


Label Type Compact URI Comment
SPT15 GeneProduct ncbigene:856891
SUA7 GeneProduct ncbigene:856201
TAF5 GeneProduct ncbigene:852497
TAF6 GeneProduct ncbigene:852766
TAF7 GeneProduct ncbigene:855267
TAF9 GeneProduct ncbigene:855276
TAF12 GeneProduct ncbigene:851723
TAF13 GeneProduct ncbigene:854875
TOA2 GeneProduct ncbigene:853807
TFG2 GeneProduct ncbigene:852888
KIN28 GeneProduct ncbigene:851450
TFB4 GeneProduct ncbigene:856169
TFB2 GeneProduct ncbigene:855981
SSL2 GeneProduct ncbigene:854663
RAD3 GeneProduct ncbigene:856918
RPA135 GeneProduct ncbigene:856119
RET1 GeneProduct ncbigene:854382
RPO21 GeneProduct ncbigene:851415
RPO26 GeneProduct ncbigene:856317
RPB3 GeneProduct ncbigene:854791
RPB5 GeneProduct ncbigene:852451
RPC25 GeneProduct ncbigene:853713
RPC11 GeneProduct ncbigene:851615
RPA190 GeneProduct ncbigene:854519
RPB7 GeneProduct ncbigene:852013
RPB8 GeneProduct ncbigene:854399
RPB11 GeneProduct ncbigene:854157
RPC19 GeneProduct ncbigene:855609
RPB2 GeneProduct ncbigene:854322
CCL1 GeneProduct ncbigene:856136
TFB3 GeneProduct ncbigene:852071


  1. Regulation of gene expression by TBP-associated proteins. Lee TI, Young RA. Genes Dev. 1998 May 15;12(10):1398–408. PubMed Europe PMC Scholia
  2. Orchestrated response: a symphony of transcription factors for gene control. Lemon B, Tjian R. Genes Dev. 2000 Oct 15;14(20):2551–69. PubMed Europe PMC Scholia