Wnt signaling pathway and pluripotency (WP399)

Homo sapiens

This pathway was adapted from several resources and is designed to provide a theoretical frame-work for examining Wnt signaling and interacting components in the context of embryonic stem-cell pluripotency and self-renewal. A central organizing theme of this pathway are known drug targets that promote self-renewal or pluripotency (BIO and IQ-1) and implicated upstream regulators of the core pluripotency transcriptional components (e.g. Nanog). It should be noted that it is unclear whether all the depicted components participate in this pathway in human embryonic stem cells. Interactions and object/gene groups for the pathway exist for the majority of components. Proteins on this pathway have targeted assays available via the [https://assays.cancer.gov/available_assays?wp_id=WP399 CPTAC Assay Portal].


Nathan Salomonis , Thomas Kelder , Martijn Van Iersel , Alex Pico , Kristina Hanspers , Daniela Digles , Egon Willighagen , Zahra Roudbari , Eric Weitz , Martina Summer-Kutmon , and Finterly Hu


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Homo sapiens



Cell Type Ontology

pluripotent stem cell

Pathway Ontology

Wnt signaling, canonical pathway Wnt signaling pathway


Label Type Compact URI Comment
IQ-1 Metabolite cas:331001-62-8
BIO Metabolite pubchem.compound:5287844
FZD8 GeneProduct ncbigene:8325
APC GeneProduct ncbigene:324
CTBP1 GeneProduct ncbigene:1487
MMP7 GeneProduct ncbigene:4316
DVL2 GeneProduct ncbigene:1856
CTNNB1 GeneProduct ncbigene:1499
WNT3A GeneProduct ncbigene:89780
JUN GeneProduct ncbigene:3725
FZD10 GeneProduct ensembl:ENSG00000111432
NFYA GeneProduct ensembl:ENSG00000001167
PRKCI GeneProduct ncbigene:5584
ESRRB GeneProduct ncbigene:2103
WNT10B GeneProduct ncbigene:7480
PLAU GeneProduct ncbigene:5328
WNT2B GeneProduct ncbigene:7482
CBP GeneProduct ncbigene:1387
PPP2CA GeneProduct ncbigene:5515
PPP2CB GeneProduct ncbigene:5516
TCF7 GeneProduct ensembl:ENSG00000081059
TCF7L2 GeneProduct ensembl:ENSG00000148737
PRKCA GeneProduct ncbigene:5578
LEF1 GeneProduct ncbigene:51176
SOX2 GeneProduct ncbigene:6657
ZBTB33 GeneProduct ncbigene:10009
RACGAP1 GeneProduct ncbigene:29127
MAPK10 GeneProduct ncbigene:5602
FZD2 GeneProduct ncbigene:2535
WNT16 GeneProduct ncbigene:51384
WNT2 GeneProduct ncbigene:7472
WNT9B GeneProduct ncbigene:7484
WNT5A GeneProduct ncbigene:7474
PPP2R1A GeneProduct ncbigene:5518
TCF7L1 GeneProduct ncbigene:83439
PPARD GeneProduct ncbigene:5467
P300 GeneProduct ncbigene:2033
PRKCQ GeneProduct ncbigene:5588
FBXW2 GeneProduct ncbigene:26190
NLK GeneProduct ncbigene:51701
PPM1J GeneProduct ensembl:ENSG00000155367
WNT1 GeneProduct ncbigene:7471
PRKCB1 GeneProduct ncbigene:5579
PRKD1 GeneProduct ncbigene:5587
FOXD3 GeneProduct ensembl:ENSG00000187140
MYC GeneProduct ncbigene:4609
WNT6 GeneProduct ncbigene:7475
DVL1 GeneProduct ncbigene:1855
PAFAH1B1 GeneProduct ncbigene:5048
MAP2K4 GeneProduct ncbigene:6416
WNT11 GeneProduct ncbigene:7481
TP53 GeneProduct ncbigene:7157
CCND1 GeneProduct ncbigene:595
WNT3 GeneProduct ncbigene:7473
PPP2R2B GeneProduct ncbigene:5521
MAPK9 GeneProduct ncbigene:5601
PPP2R3B GeneProduct ncbigene:28227
POU5F1 GeneProduct ncbigene:5460
WNT7A GeneProduct ncbigene:7476
CTBP2 GeneProduct ncbigene:1488
PPP2R1B GeneProduct ncbigene:5519
PPP2R5E GeneProduct ncbigene:5529
PRKCZ GeneProduct ncbigene:5590
LRP6 GeneProduct ncbigene:4040
FZD4 GeneProduct ncbigene:8322
DVL3 GeneProduct ncbigene:1857
Prkcc GeneProduct ncbigene:5582
FOSL1 GeneProduct ncbigene:8061
AXIN2 GeneProduct ncbigene:8313
FZD9 GeneProduct ncbigene:8326
RHOA GeneProduct ncbigene:387
FZD6 GeneProduct ncbigene:8323
NKD1 GeneProduct ncbigene:85407
FZD7 GeneProduct ncbigene:8324
NKD2 GeneProduct ncbigene:85409
FRAT1 GeneProduct ncbigene:10023
AXIN1 GeneProduct ncbigene:8312
NANOG GeneProduct ncbigene:79923
PPP2R5C GeneProduct ncbigene:5527
GSK3B GeneProduct ncbigene:2932
WNT4 GeneProduct ncbigene:54361
PPP2R2A GeneProduct ncbigene:5520
CSNK1E GeneProduct ncbigene:1454
PPP2R2C GeneProduct ncbigene:5522
FZD3 GeneProduct ncbigene:7976
CTNND1 GeneProduct ncbigene:1500
WNT10A GeneProduct ncbigene:80326
LDLR GeneProduct ncbigene:3949
MAP3K7 GeneProduct ncbigene:6885
WNT11 GeneProduct ncbigene:7481 Non-canonical Wnt signaling enhances differentiation of human circulating progenitor cells to cardiomyogenic cells. PMID: 15701629
PRKCE GeneProduct ncbigene:5581
FZD5 GeneProduct ncbigene:7855
CCND3 GeneProduct ncbigene:896
PPP2R3A GeneProduct ncbigene:5523
PRKCH GeneProduct ncbigene:5583
CD44 GeneProduct ncbigene:960
WNT7B GeneProduct ncbigene:7477
WNT5B GeneProduct ncbigene:81029
FZD1 GeneProduct ncbigene:8321
PRKCD GeneProduct ncbigene:5580
PPP2R4 GeneProduct ncbigene:5524
LRP5 GeneProduct ncbigene:4041
CCND2 GeneProduct ncbigene:894
LRRK2-mut GeneProduct ncbigene:120892
CTNNB1 GeneProduct ncbigene:1499
CTNNB1 GeneProduct ncbigene:1499


  1. KEGG Pathway: hsa04310
  2. Manipulation of self-renewal in human embryonic stem cells through a novel pharmacological GSK-3 inhibitor. Sato N, Brivanlou AH. Methods Mol Biol. 2006;331:115–28. PubMed Europe PMC Scholia
  3. Wnt/beta-catenin/CBP signaling maintains long-term murine embryonic stem cell pluripotency. Miyabayashi T, Teo JL, Yamamoto M, McMillan M, Nguyen C, Kahn M. Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5668–73. PubMed Europe PMC Scholia
  4. Tcf3 functions as a steady-state limiter of transcriptional programs of mouse embryonic stem cell self-renewal. Yi F, Pereira L, Merrill BJ. Stem Cells. 2008 Aug;26(8):1951–60. PubMed Europe PMC Scholia
  5. Esrrb activates Oct4 transcription and sustains self-renewal and pluripotency in embryonic stem cells. Zhang X, Zhang J, Wang T, Esteban MA, Pei D. J Biol Chem. 2008 Dec 19;283(51):35825–33. PubMed Europe PMC Scholia
  6. (G2019S) LRRK2 activates MKK4-JNK pathway and causes degeneration of SN dopaminergic neurons in a transgenic mouse model of PD. Chen CY, Weng YH, Chien KY, Lin KJ, Yeh TH, Cheng YP, et al. Cell Death Differ. 2012 Oct;19(10):1623–33. PubMed Europe PMC Scholia