Meiotic recombination genes (WP377)

Saccharomyces cerevisiae

Meiotic double strand breaks are initiated by Spo11, a conserved topoisomerase-derived protein, together with its partner subunits. After the breaks are formed, Spo11 remains covalently attached to the 5 prime strands at both ends of the DNA. It is released through an endonucleolytic cleavage reaction, which is facilitated by MRX (Mre11, Rad50, and Xrs2) and Sae2. This reaction liberates Spo11, which is attached to a short oligonucleotide. The 5 prime strands are further processed by exonucleases, such as Exo1 in yeast, leading to the production of long single-stranded tails. These tails are coated with RPA, an ssDNA-binding protein, before being replaced by recombinases Rad51 and Dmc1. The recombinases form a nucleoprotein filament and search for sequence similarity, which is primarily located on the homologous chromosome. This process leads to the production of D-loop structures.

Authors

Alex Pico and Eric Weitz

Activity

last edited

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Organisms

Saccharomyces cerevisiae

Communities

Annotations

Pathway Ontology

cell cycle pathway, meiotic regulatory pathway

Participants

Label Type Compact URI Comment
MEK1 GeneProduct sgd:S000005878
RAD51 GeneProduct sgd:S000000897
SPO11 GeneProduct sgd:S000001014
REC114 GeneProduct sgd:S000004740
RED1 GeneProduct sgd:S000004253
HOP1 GeneProduct sgd:S000001334
RAD50 GeneProduct sgd:S000005194
RAD57 GeneProduct sgd:S000002411
MER2 GeneProduct sgd:S000003782
MEI4 GeneProduct sgd:S000001954
XRS2 GeneProduct sgd:S000002777
RAD52 GeneProduct sgd:S000004494
REC104 GeneProduct sgd:S000001200
MRE11 GeneProduct sgd:S000004837
RAD55 GeneProduct sgd:S000002483
REC102 GeneProduct sgd:S000004321

References