Acetylcholine synthesis (WP360)

Rattus norvegicus

Acetylcholine is an important neurotransmitter. It can be rapidly released in the synaptic cleft upon activation of the neuron. In the synaptic cleft the compound is degraded rapidly into choline and acetate, this is essential for proper neuronal functioning. Choline and Acetate are taken up into the cytosol and recycled for the next activation.


Andrew Kwa , Manny Ramirez , Kristina Hanspers , Thomas Kelder , Andra Waagmeester , Christine Chichester , Egon Willighagen , Lauren J. Dupuis , and Eric Weitz


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Rattus norvegicus



Pathway Ontology

acetylcholine metabolic pathway


Label Type Compact URI Comment
Phosphatidylcholine Metabolite chebi:49183
Pyruvate from Glycolysis Metabolite chebi:15361
Phosphorylcholine Metabolite hmdb:HMDB0001565
Acetate Metabolite chebi:30089
Acetylcholine Metabolite hmdb:HMDB0000895
Acetyl-CoA Metabolite hmdb:HMDB0001206
Cytidine Diphosphate Choline Metabolite chebi:16436
Phosphatidylethanolamine Metabolite chebi:16038
Choline Metabolite hmdb:HMDB0000097
Choline Metabolite hmdb:HMDB0000097
Glycerophosphocholine Metabolite chebi:16870
Pcyt1a GeneProduct ncbigene:140544
Pdha1 GeneProduct ncbigene:29554
Ahsg GeneProduct ncbigene:25373
Ache GeneProduct ncbigene:83817
Chat GeneProduct uniprot:CLAT
Pemt GeneProduct ncbigene:25511
Pdha2 GeneProduct ncbigene:117098


  1. Choline and cholinergic neurons. Blusztajn JK, Wurtman RJ. Science. 1983 Aug 12;221(4611):614–20. PubMed Europe PMC Scholia