RuMP cycle, oxidative branch of the pentose phosphate pathway and formaldehyde assimilation (WP332)

Saccharomyces cerevisiae

The reactions of the oxidative branch of the pentose phosphate pathway occur in the cytosol and are important for generating NADPH through the oxidative reduction of NADP+. NADPH is an important source of the reducing energy required by many enzymes in central biosynthetic pathways. Although there are other biochemical pathways that generate NADPH in yeast, the pentose phosphate pathway is considered the major source of NADPH, and insufficient NADPH probably contributes to the fitness defects seen in mutants of the oxidative branch of the pentose phosphate pathway.


Jessica Heckman , Daniela Digles , Egon Willighagen , and Denise Slenter


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Saccharomyces cerevisiae



Pathway Ontology

RuMP pathway of formaldehyde assimilation pentose phosphate pathway - oxidative phase


Label Type Compact URI Comment
fructose-6-phosphate Metabolite cas:643-13-0
S-hydroxymethylglutathione Metabolite chebi:48926
NADPH Metabolite cas:53-57-6
S-formylglutathione Metabolite chebi:16225
NADH Metabolite cas:53-84-9
D-arabino-3-Hexulose 6-phosphate Metabolite chebi:27973
CO2 Metabolite chebi:16526
6-phosphono-D-glucono-lactone Metabolite chebi:16938
NADH Metabolite cas:53-84-9
formaldehyde Metabolite cas:50-00-0
H2O Metabolite chebi:15377
6-phospho-D-gluconate Metabolite chebi:58759
formate Metabolite chebi:15740
NADPH Metabolite chebi:16474
ribulose-5-phosphate Metabolite cas:4151-19-3
glucose-6-phosphate Metabolite cas:59-56-3
glutathione Metabolite chebi:57925
NAD Metabolite chebi:13389
CO2 Metabolite chebi:16526
NADP Metabolite chebi:44409
H+ Metabolite chemspider:1010
NADPH Metabolite chebi:16474
H+ Metabolite chebi:15378
H+ Metabolite chemspider:1010
H+ Metabolite chemspider:1010
NADP Metabolite chebi:44409
glutathione Metabolite chebi:57925
H2O Metabolite chebi:15377
FDH1 GeneProduct sgd:S000005915
SOL3 GeneProduct ensembl:YHR163W
GND1 GeneProduct sgd:S000001226
SOL4 GeneProduct ensembl:YGR248W
ZWF1 GeneProduct ensembl:YNL241C
GND2 GeneProduct sgd:S000003488
SFA1 GeneProduct ensembl:YDL168W
S-formylglutathione hydrolase Protein uniprot:P40363


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