RuMP cycle, oxidative branch of the pentose phosphate pathway and formaldehyde assimilation (WP332)

Saccharomyces cerevisiae

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The reactions of the oxidative branch of the pentose phosphate pathway occur in the cytosol and are important for generating NADPH through the oxidative reduction of NADP+. NADPH is an important source of the reducing energy required by many enzymes in central biosynthetic pathways. Although there are other biochemical pathways that generate NADPH in yeast, the pentose phosphate pathway is considered the major source of NADPH, and insufficient NADPH probably contributes to the fitness defects seen in mutants of the oxidative branch of the pentose phosphate pathway.

Authors

Jessica Heckman , Daniela Digles , Egon Willighagen , and Denise Slenter

Activity

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Organisms

Saccharomyces cerevisiae

Communities

Annotations

Pathway Ontology

RuMP pathway of formaldehyde assimilation pentose phosphate pathway - oxidative phase

Participants

Label Type Compact URI Comment
fructose-6-phosphate Metabolite cas:643-13-0
S-hydroxymethylglutathione Metabolite chebi:48926
NADPH Metabolite cas:53-57-6
S-formylglutathione Metabolite chebi:16225
NADH Metabolite cas:53-84-9
D-arabino-3-Hexulose 6-phosphate Metabolite chebi:27973
CO2 Metabolite chebi:16526
6-phosphono-D-glucono-lactone Metabolite chebi:16938
NADH Metabolite cas:53-84-9
formaldehyde Metabolite cas:50-00-0
H2O Metabolite chebi:15377
6-phospho-D-gluconate Metabolite chebi:58759
formate Metabolite chebi:15740
NADPH Metabolite chebi:16474
ribulose-5-phosphate Metabolite cas:4151-19-3
glucose-6-phosphate Metabolite cas:59-56-3
glutathione Metabolite chebi:57925
NAD Metabolite chebi:13389
CO2 Metabolite chebi:16526
NADP Metabolite chebi:44409
H+ Metabolite chemspider:1010
NADPH Metabolite chebi:16474
H+ Metabolite chebi:15378
H+ Metabolite chemspider:1010
H+ Metabolite chemspider:1010
NADP Metabolite chebi:44409
glutathione Metabolite chebi:57925
H2O Metabolite chebi:15377
FDH1 GeneProduct sgd:S000005915
SOL3 GeneProduct ensembl:YHR163W
GND1 GeneProduct sgd:S000001226
SOL4 GeneProduct ensembl:YGR248W
ZWF1 GeneProduct ensembl:YNL241C
GND2 GeneProduct sgd:S000003488
SFA1 GeneProduct ensembl:YDL168W
S-formylglutathione hydrolase Protein uniprot:P40363

References

  1. Induction of specific enzymes of the oxidative pentose phosphate pathway by glucono-delta-lactone in Saccharomyces cerevisiae. Sinha A, Maitra PK. J Gen Microbiol. 1992 Sep;138(9):1865–73. PubMed Europe PMC Scholia
  2. Isolation and characterization of the ZWF1 gene of Saccharomyces cerevisiae, encoding glucose-6-phosphate dehydrogenase. Nogae I, Johnston M. Gene. 1990 Dec 15;96(2):161–9. PubMed Europe PMC Scholia
  3. Overexpression of ADH1 confers hyper-resistance to formaldehyde in Saccharomyces cerevisiae. Grey M, Schmidt M, Brendel M. Curr Genet. 1996 Apr;29(5):437–40. PubMed Europe PMC Scholia
  4. Purification and properties of an esterase from the yeast Saccharomyces cerevisiae and identification of the encoding gene. Degrassi G, Uotila L, Klima R, Venturi V. Appl Environ Microbiol. 1999 Aug;65(8):3470–2. PubMed Europe PMC Scholia
  5. Enhanced formaldehyde detoxification by overexpression of glutathione-dependent formaldehyde dehydrogenase from Arabidopsis. Achkor H, Díaz M, Fernández MR, Biosca JA, Parés X, Martínez MC. Plant Physiol. 2003 Aug;132(4):2248–55. PubMed Europe PMC Scholia
  6. Division of labor among the yeast Sol proteins implicated in tRNA nuclear export and carbohydrate metabolism. Stanford DR, Whitney ML, Hurto RL, Eisaman DM, Shen WC, Hopper AK. Genetics. 2004 Sep;168(1):117–27. PubMed Europe PMC Scholia