RNA interference (WP3164)

Bos taurus

The process of RNA-interference in eukaryotic cells. Long precursor microRNA (miRNA), called pri-miRNA, is cleaved by RNase III endonuclease (Drosha) into pieces of approximately 70 nucleotides each (called pre-miRNA) in the nucleus. Following transportation into the cytoplasm by exportin 5 another RNase III endonuclease (Dicer) cleaves it into mature miRNA segments. Degradation of messenger RNA (mRNA) and translational repression occurs after miRNA binds to the RNA-induced silencing complex (RISC). Cytoplasmic long double-stranded RNA (dsRNA) is cleaved by Dicer into small interfering RNA (siRNA), which is incorporated into RISC, resulting in the cleavage and degradation of specific target mRNA. Synthetic double-stranded siRNA is not processed by Dicer and directly incorporated by the RISC.

Authors

Martina Summer-Kutmon

Activity

last edited

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Organisms

Bos taurus

Communities

Annotations

Pathway Ontology

RNA degradation pathway

Participants

Label Type Compact URI Comment
DICER1 GeneProduct ensembl:ENSBTAG00000012852 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:23405
XPO5 GeneProduct ensembl:ENSBTAG00000018970 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:57510
DROSHA GeneProduct ensembl:ENSBTAG00000017551 HomologyConvert: Homo sapiens to Bos taurus: Original ID = L:29102
DGCR8 GeneProduct ensembl:ENSBTAG00000019869 HomologyConvert: Homo sapiens to Bos taurus: Original ID = En:ENSG00000128191
ERI1 GeneProduct ensembl:ENSBTAG00000048265 HomologyConvert: Homo sapiens to Bos taurus: Original ID = En:ENSG00000104626

References

  1. Liposomal siRNA nanocarriers for cancer therapy. Ozpolat B, Sood AK, Lopez-Berestein G. Adv Drug Deliv Rev. 2014 Feb;66:110–6. PubMed Europe PMC Scholia
  2. Eri1: a conserved enzyme at the crossroads of multiple RNA-processing pathways. Thomas MF, L’Etoile ND, Ansel KM. Trends Genet. 2014 Jul;30(7):298–307. PubMed Europe PMC Scholia