Brassinolide biosynthetic pathway (WP2634)

Arabidopsis thaliana

Brassinolide is a plant hormone.


Andra Waagmeester , Egon Willighagen , Anders Riutta , Denise Slenter , Finterly Hu , and Conroy lipids


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Arabidopsis thaliana



Pathway Ontology

lipid metabolic pathway classic metabolic pathway


Label Type Compact URI Comment
Castasterone Metabolite lipidmaps:LMST01030129
5-dehydroepisterol Metabolite lipidmaps:LMST01030135
campest-4-en-3-one Metabolite lipidmaps:LMST01030122
Brassinolide Metabolite lipidmaps:LMST01140001
Episterol Metabolite lipidmaps:LMST01030115
Cathasterone Metabolite lipidmaps:LMST01030132
6α-Hydroxycastasterone Metabolite lipidmaps:LMST01030128
6-Oxocampestanol Metabolite lipidmaps:LMST01030133
3-Dehydroteasterone Metabolite lipidmaps:LMST01030131
Typhasterol Metabolite lipidmaps:LMST01030130
Teasterone Metabolite lipidmaps:LMST01030121
6-Deoxocastasterone Metabolite lipidmaps:LMST01030127
6-Deoxotyphasterol Metabolite lipidmaps:LMST01030126
3-Dehydro-6-deoxoteasterone Metabolite lipidmaps:LMST01030125
6-Deoxoteasterone Metabolite lipidmaps:LMST01030120
6-Deoxocathasterone Metabolite lipidmaps:LMST01030124
Campestanol Metabolite lipidmaps:LMST01030103
6α-Hydroxycampestanol Metabolite lipidmaps:LMST01030134
5α-campestan-3-one Metabolite lipidmaps:LMST01030123
campest-4-en-3beta-ol Metabolite lipidmaps:LMST01031016
Campesterol Metabolite lipidmaps:LMST01030097
24-Methylenecholesterol Metabolite lipidmaps:LMST01030099
dwf4 GeneProduct ensembl:AT3G50660
CYP85A2 GeneProduct uniprot:Q940V4
CYP90B1 GeneProduct uniprot:O64989
STE1 GeneProduct uniprot:Q39208
dwf4 GeneProduct ensembl:AT3G50660
BRI1 GeneProduct ensembl:AT4G39400 Homologous to lka (pea) and cu-3 (tomato)?
cpd GeneProduct ensembl:AT5G05690 Potentially relevant?
Homologous to dpy (tomato)?
DET2 GeneProduct ensembl:AT2G38050 Homologous to lk (pea)?
Synonym: dwf6
cpd GeneProduct ensembl:AT5G05690 Synonyms: cbb3, dwf3


  1. The Arabidopsis dwf7/ste1 mutant is defective in the delta7 sterol C-5 desaturation step leading to brassinosteroid biosynthesis. Choe S, Noguchi T, Fujioka S, Takatsuto S, Tissier CP, Gregory BD, et al. Plant Cell. 1999 Feb;11(2):207–21. PubMed Europe PMC Scholia
  2. Arabidopsis det2 is defective in the conversion of (24R)-24-methylcholest-4-En-3-one to (24R)-24-methyl-5alpha-cholestan-3-one in brassinosteroid biosynthesis. Noguchi T, Fujioka S, Takatsuto S, Sakurai A, Yoshida S, Li J, et al. Plant Physiol. 1999 Jul;120(3):833–40. PubMed Europe PMC Scholia
  3. A putative role for the tomato genes DUMPY and CURL-3 in brassinosteroid biosynthesis and response. Koka CV, Cerny RE, Gardner RG, Noguchi T, Fujioka S, Takatsuto S, et al. Plant Physiol. 2000 Jan;122(1):85–98. PubMed Europe PMC Scholia
  4. Brassinosteroid-6-oxidases from Arabidopsis and tomato catalyze multiple C-6 oxidations in brassinosteroid biosynthesis. Shimada Y, Fujioka S, Miyauchi N, Kushiro M, Takatsuto S, Nomura T, et al. Plant Physiol. 2001 Jun;126(2):770–9. PubMed Europe PMC Scholia
  5. Arabidopsis CYP85A2, a cytochrome P450, mediates the Baeyer-Villiger oxidation of castasterone to brassinolide in brassinosteroid biosynthesis. Kim TW, Hwang JY, Kim YS, Joo SH, Chang SC, Lee JS, et al. Plant Cell. 2005 Aug;17(8):2397–412. PubMed Europe PMC Scholia