Glycolysis (WP253)

Saccharomyces cerevisiae

Many yeast and bacterial species ferment pyruvate to 2,3-butanediol via the intermediate, acetoin. Although acetoin is a flavorless compound, it is a precursor to some off-odor compounds, such as diacetyl, which can be unfavorable in beer and wine. Acetoin and 2,3-butanediol are both by-products of alcoholic fermentation by S. cerevisiae and other wine yeasts. Acetoin production during alcoholic fermentation depends on the yeast strain and other variables such as substrate and temperature (as reported in (CITS: [16535224])). Wine yeasts with high fermentation power generally produce low acetoin levels and large amounts of ethanol and higher alcohols (alcohols with more than 2 carbons) (as reported in (CITS: [16535224])). SOURCE: SGD pathways,


Jessica Heckman , Christine Chichester , Egon Willighagen , Denise Slenter , and Marvin Martens


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Saccharomyces cerevisiae



Pathway Ontology

glycolysis pathway


Label Type Compact Identifier Comment
H+ Metabolite chebi:15378
H+ Metabolite chebi:15378
dihydroxy-acetone-phosphate Metabolite hmdb:HMDB0001473
H+ Metabolite chebi:15378
fructose-1,6-bisphosphate Metabolite hmdb:HMDB0001058
2-phosphoglycerate Metabolite chebi:24344
H2O Metabolite chebi:15377
pyruvate Metabolite chebi:15361
NAD Metabolite chebi:13389
3-Phosphoglycerate Metabolite chebi:17050
fructose-6-phosphate (closed form) Metabolite chemspider:26330295
glyceraldehyde-3-phosphate Metabolite cas:591-57-1
NADH Metabolite cas:53-84-9
ADP Metabolite cas:58-64-0
ADP Metabolite cas:58-64-0
ATP Metabolite cas:1927-31-7
glucose-6-phosphate (closed form) Metabolite chebi:75150
ATP Metabolite cas:1927-31-7
ATP Metabolite cas:1927-31-7
3-phospho-D-glyceroyl-phosphate Metabolite cas:38168-82-0
phosphoenolpyruvate Metabolite cas:138-08-9
Phosphate Metabolite cas:14265-44-2
ADP Metabolite cas:58-64-0
Glucose-6P (open) Metabolite chebi:57584 D-Glucose-6-Phosphate (2-) open form
Fructose 6P (open) Metabolite chebi:57579 fructose-6-phosphate (2-) open form
CDC19 GeneProduct ensembl:YAL038W pyruvate kinase 1
TPI1 GeneProduct ensembl:YDR050C
ERR1 GeneProduct ensembl:YOR393W enolase: ERR1
PFK2 GeneProduct ensembl:YMR205C
GPM3 GeneProduct ensembl:YOL056W
ENO2 GeneProduct ensembl:YHR174W
FBA1 GeneProduct ensembl:YKL060C
phosphopyruvate hydratase GeneProduct sgd:S000004942
PGK1 GeneProduct ensembl:YCR012W 3-phophsoglycerate kinase
ENO1 GeneProduct ensembl:YGR254W
TDH2 GeneProduct ensembl:YJR009C glyceraldehyde 3-phosphate dehydrogenase 2
PYK2 GeneProduct ensembl:YOR347C pyruvate kinase 2
TDH1 GeneProduct ensembl:YJL052W glyceraldehyde 3-phosphate dehydrogenase 1
PGI1 GeneProduct ensembl:YBR196C glucose-6-phosphate isomerase
GPM1 GeneProduct ensembl:YKL152C phosphoglycerate mutase 1
PFK1 GeneProduct ensembl:YGR240C
TDH3 GeneProduct ensembl:YGR192C glyceraldehyde 3-phosphate dehydrogenase 3
ERR2 GeneProduct ensembl:YPL281C enolase: ERR2