Eukaryotic transcription initiation (WP177)

Drosophila melanogaster

In eukaryotes, RNA polymerase, and therefore the initiation of transcription, requires the presence of a core promoter sequence in the DNA. RNA polymerase is able to bind to core promoters in the presence of various specific transcription factors. The most common type of core promoter in eukaryotes is a short DNA sequence known as a TATA box. The TATA box, as a core promoter, is the binding site for a transcription factor known as TATA binding protein (TBP), which is itself a subunit of another transcription factor, called Transcription Factor II D (TFIID). After TFIID binds to the TATA box via the TBP, five more transcription factors and RNA polymerase combine around the TATA box in a series of stages to form a preinitiation complex. One transcription factor, DNA helicase, has helicase activity and so is involved in the separating of opposing strands of double-stranded DNA to provide access to a single-stranded DNA template. However, only a low, or basal, rate of transcription is driven by the preinitiation complex alone. Other proteins known as activators and repressors, along with any associated coactivators or corepressors, are responsible for modulating transcription rate. Source: [[wikipedia:Transcription_(genetics)|Wikipedia]]


Nathan Salomonis , Thomas Kelder , Christine Chichester , Kristina Hanspers , Ryan Miller , Egon Willighagen , and Eric Weitz


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Drosophila melanogaster



Pathway Ontology

RNA polymerase III transcription initiation pathway RNA polymerase I transcription initiation pathway RNA polymerase II transcription initiation pathway


Label Type Compact URI Comment
Tbp GeneProduct ncbigene:37476
TfIIB GeneProduct ncbigene:34430
Taf5 GeneProduct ncbigene:47900
Taf6 GeneProduct ncbigene:40134
Taf7 GeneProduct ncbigene:40934
Ilk GeneProduct ncbigene:53573
Taf12 GeneProduct ncbigene:41406
Taf13 GeneProduct ncbigene:35297
TfIIEalpha GeneProduct ncbigene:39313
TfIIA-S GeneProduct ncbigene:42822
TfIIFbeta GeneProduct ncbigene:41290
TfIIEbeta GeneProduct ncbigene:38527
Cdk7 GeneProduct ncbigene:31441
Tfb1 GeneProduct ncbigene:36598
Tfb4 GeneProduct ncbigene:33294
Tfb2 GeneProduct ncbigene:39688
hay GeneProduct ncbigene:39202
Xpd GeneProduct ncbigene:37414
RpI135 GeneProduct ncbigene:33210
RpIII128 GeneProduct ncbigene:36289
RpII215 GeneProduct ncbigene:32100
RpII18 GeneProduct ncbigene:48312
RpII33 GeneProduct ncbigene:34796
Rpb5 GeneProduct ncbigene:36160
Sin GeneProduct ncbigene:40325
CG7339 GeneProduct ncbigene:39310
CG33785 GeneProduct ncbigene:3772344
RpI1 GeneProduct ncbigene:36617
RpII15 GeneProduct ncbigene:41741
Rpb7 GeneProduct ncbigene:261631
Rpb8 GeneProduct ncbigene:40415
Rpb11 GeneProduct ncbigene:35043
RpII140 GeneProduct ncbigene:41721
CycH GeneProduct ncbigene:40429
Mat1 GeneProduct ncbigene:36130


  1. Regulation of gene expression by TBP-associated proteins. Lee TI, Young RA. Genes Dev. 1998 May 15;12(10):1398–408. PubMed Europe PMC Scholia
  2. Orchestrated response: a symphony of transcription factors for gene control. Lemon B, Tjian R. Genes Dev. 2000 Oct 15;14(20):2551–69. PubMed Europe PMC Scholia