mRNA processing (WP142)

Drosophila melanogaster

This process describes the conversion of precursor messenger RNA into mature messenger RNA (mRNA). The pre-mRNA molecule undergoes three main modifications. These modifications are 5' capping, 3' polyadenylation, and RNA splicing, which occur in the cell nucleus before the RNA is translated. 5' Capping: Capping of the pre-mRNA involves the addition of 7-methylguanosine (m7G) to the 5' end. The cap protects the 5' end of the primary RNA transcript from attack by ribonucleases that have specificity to the 3'5' phosphodiester bonds. 3' Processing: The pre-mRNA processing at the 3' end of the RNA molecule involves cleavage of its 3' end and then the addition of about 200 adenine residues to form a poly(A) tail. As the poly(A) tails is synthesised, it binds multiple copies of poly(A) binding protein, which protects the 3'end from ribonuclease digestion. Splicing: RNA splicing is the process by which introns, regions of RNA that do not code for protein, are removed from the pre-mRNA and the remaining exons connected to re-form a single continuous molecule. Description adapted from Wikipedia:


Nathan Salomonis , Kristina Hanspers , Daniela Digles , and Alex Pico


last edited

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Drosophila melanogaster



Pathway Ontology

spliceosome pathway


Label Type Compact URI Comment
GMP Metabolite hmdb:HMDB0001397
ATP Metabolite cas:1927-31-7
Srp54 GeneProduct ncbigene:34312 PMID: 14559993. SRrp86 is a unique member of the SR protein superfamily containing one RNA recognition motif and two serine-arginine (SR)-rich domains separated by an unusual glutamic acid-lysine (EK)-rich region. Previously, we showed that SRrp86 could regulate alternative splicing by both positively and negatively modulating the activity of other SR proteins and that the unique EK domain could inhibit both constitutive and alternative splicing. These functions were most consistent with the model in which SRrp86 functions by interacting with and thereby modulating the activity of target proteins. To identify the specific proteins that interact with SRrp86, we used a yeast two-hybrid library screen and immunoprecipitation coupled to mass spectrometry. We show that SRrp86 interacts with all of the core SR proteins, as well as a subset of other splicing regulatory proteins, including SAF-B, hnRNP G, YB-1, and p72. In contrast to previous results that showed activation of SRp20 by SRrp86, we now show that SAF-B, hnRNP G, and 9G8 all antagonize the activity of SRrp86. Overall, we conclude that not only does SRrp86 regulate SR protein activity but that it is, in turn, regulated by other splicing factors to control alternative splice site selection.
CG3605 GeneProduct ncbigene:33514
SRm160 GeneProduct ncbigene:39483
CG5970 GeneProduct ncbigene:36494
CG6841 GeneProduct ncbigene:40064
cpsf GeneProduct ncbigene:44250
Cbp80 GeneProduct ncbigene:44409
CstF-50 GeneProduct ncbigene:43734
hrg GeneProduct ncbigene:49636
bai GeneProduct ncbigene:42996
xl6 GeneProduct ncbigene:33967
CG11266 GeneProduct ncbigene:33989
CG10418 GeneProduct ncbigene:39408
CG10689 GeneProduct ncbigene:35192
bru-3 GeneProduct ncbigene:39527
CG8877 GeneProduct ncbigene:36304
CG1249 GeneProduct ncbigene:40781
U2A GeneProduct ncbigene:35713
CG7698 GeneProduct ncbigene:42240
CG6015 GeneProduct ncbigene:42593
CG3689 GeneProduct ncbigene:39083
CG9548 GeneProduct ncbigene:33909
snRNP69D GeneProduct ncbigene:44668
Spx GeneProduct ncbigene:31558
CG16941 GeneProduct ncbigene:42048
DebB GeneProduct ncbigene:36314
Hrb87F GeneProduct ncbigene:48535
Art8 GeneProduct ncbigene:34528
CstF-64 GeneProduct ncbigene:42239
Spt5 GeneProduct ncbigene:53442
CG17540 GeneProduct ncbigene:3355114
noi GeneProduct ncbigene:40678
CG6322 GeneProduct ncbigene:39955
SmB GeneProduct ncbigene:34426
CG6695 GeneProduct ncbigene:42937
CG1957 GeneProduct ncbigene:43426
CG9924 GeneProduct ncbigene:41704
bru-2 GeneProduct ncbigene:250811
CG7757 GeneProduct ncbigene:40172
snf GeneProduct ncbigene:31442
CG13277 GeneProduct ncbigene:35003
Pabp2 GeneProduct ncbigene:35788
CG17838 GeneProduct ncbigene:42460
CG6322 GeneProduct ncbigene:39955
mle GeneProduct ncbigene:35523
CG5198 GeneProduct ncbigene:34432
SF2 GeneProduct ncbigene:53443 Antagonist splicing factor
CG11985 GeneProduct ncbigene:50007
Dcr-1 GeneProduct ncbigene:42693
mRNA-capping-enzyme GeneProduct ncbigene:32379
heph GeneProduct ncbigene:48571
su(f) GeneProduct ncbigene:3354994
CG10354 GeneProduct ncbigene:33964
CG10375 GeneProduct ncbigene:42812
CG4896 GeneProduct ncbigene:33305
SC35 GeneProduct ncbigene:53444
l(1)G0007 GeneProduct ncbigene:32373
RpII215 GeneProduct ncbigene:32100
sbr GeneProduct ncbigene:43944
aret GeneProduct ncbigene:34648
snRNP70K GeneProduct ncbigene:33982
Doa GeneProduct ncbigene:43415
CG8241 GeneProduct ncbigene:36561
yps GeneProduct ncbigene:39377
CG10754 GeneProduct ncbigene:39456
Ddx1 GeneProduct ncbigene:40457
caz GeneProduct ncbigene:32587
CG4849 GeneProduct ncbigene:43358
sm GeneProduct ncbigene:37254
SmD3 GeneProduct ncbigene:36306
CG2807 GeneProduct ncbigene:33235
CG7028 GeneProduct ncbigene:38032
CG9742 GeneProduct ncbigene:32636
CG3436 GeneProduct ncbigene:33180
l(2)35Bd GeneProduct ncbigene:34877
CG11107 GeneProduct ncbigene:35654
CG10418 GeneProduct ncbigene:39408
CG8877 GeneProduct ncbigene:36304
CG18591 GeneProduct ncbigene:34080
CG9373 GeneProduct ncbigene:41138
Clp GeneProduct ncbigene:33259
SMC1 GeneProduct ncbigene:42853
CG6015 GeneProduct ncbigene:42593
U2af50 GeneProduct ncbigene:32602
Rbp1 GeneProduct ncbigene:41294
B52 GeneProduct ncbigene:41670
CG3058 GeneProduct ncbigene:33645
CG13900 GeneProduct ncbigene:38093