Wnt Signaling Pathway and Pluripotency (WP1344)

Danio rerio

This pathway was adapted from several resources and is designed to provide a theoretical frame-work for examining Wnt signaling and interacting components in the context of embryonic stem-cell pluripotency and self-renewal. A central organizing theme of this pathway are known drug targets which promote self-renewal or pluripotency (BIO and IQ-1) and implicated upstream regulators of the core pluripotency transcriptional components (e.g. Nanog). It should be noted that it is unclear whether all the depicted components participate in this pathway in human embryonic stem cells. Interactions and object/gene groups for the pathway exist for the majority of components.


Kristina Hanspers , Martina Summer-Kutmon , Alex Pico , and Egon Willighagen


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Danio rerio



Pathway Ontology

Wnt signaling pathway

Cell Type Ontology

pluripotent stem cell


Label Type Compact URI Comment
apc GeneProduct ncbigene:386762
zgc:136929 GeneProduct ncbigene:678649
dvl2 GeneProduct ncbigene:323630
ctnnb1 GeneProduct ncbigene:30265
wnt3a GeneProduct ncbigene:60632
jun GeneProduct ncbigene:335916
prkci GeneProduct ncbigene:117507
wnt10b GeneProduct ncbigene:30308
wnt2bb GeneProduct ncbigene:556087
zgc:56064 GeneProduct ncbigene:393885
ppp2cb GeneProduct ncbigene:406582
prkca GeneProduct ncbigene:497384
lef1 GeneProduct ncbigene:30701
sox2 GeneProduct ncbigene:378723
zbtb33 GeneProduct ncbigene:562507
racgap1 GeneProduct ncbigene:323197
mapk10 GeneProduct ncbigene:569698
fzd2 GeneProduct ncbigene:30370
wnt16 GeneProduct ncbigene:791563
wnt2ba GeneProduct ncbigene:359837
wnt9b GeneProduct ncbigene:565677
wnt5a GeneProduct ncbigene:568191
foxj1a GeneProduct ncbigene:767737
gpc4 GeneProduct ncbigene:118437
prkcq GeneProduct ncbigene:555521
fbxw2 GeneProduct ncbigene:553942
wnt1 GeneProduct ncbigene:30128
prkcbb GeneProduct ncbigene:393953
prkd1 GeneProduct ncbigene:557123
LOC561831 GeneProduct ncbigene:561831
dvl1b GeneProduct ncbigene:560032
pafah1b1b GeneProduct ncbigene:394247
wnt11r GeneProduct ncbigene:30283
tp53 GeneProduct ncbigene:30590
ccnd1 GeneProduct ncbigene:30222
wnt3 GeneProduct ncbigene:569420
ppp2r2bb GeneProduct ncbigene:558810
mapk9 GeneProduct ncbigene:100149273
si:ch211-269e2.2 GeneProduct ncbigene:566032
wnt7aa GeneProduct ncbigene:565714
ppp2r1b GeneProduct ncbigene:449548
ppp2r5eb GeneProduct ncbigene:373878
prkcz GeneProduct ncbigene:555737
lrp6 GeneProduct ncbigene:100003877
LOC100333651 GeneProduct ncbigene:100333651
LOC100149082 GeneProduct ncbigene:100149082
fosl1 GeneProduct ncbigene:564241
axin2 GeneProduct ncbigene:58080
zgc:153713 GeneProduct ncbigene:751630
fzd6 GeneProduct ncbigene:393533
nkd1 GeneProduct ncbigene:751108
nkd2b GeneProduct ncbigene:794221
axin1 GeneProduct ncbigene:57931
ppp2r5c GeneProduct ncbigene:557208
gsk3b GeneProduct ncbigene:30654
wnt4a GeneProduct ncbigene:30123
ppp2r2d GeneProduct ncbigene:327263
csnk1da GeneProduct ncbigene:322106
LOC100335012 GeneProduct ncbigene:100335012
fzd3a GeneProduct ncbigene:565921
wnt10a GeneProduct ncbigene:30171
ldlr GeneProduct ncbigene:387529
map3k7 GeneProduct ncbigene:553788
wnt11r GeneProduct ncbigene:30283 Non-canonical Wnt signaling enhances differentiation of human circulating progenitor cells to cardiomyogenic cells. PMID: 15701629
prkcea GeneProduct ncbigene:100005075
fzd5 GeneProduct ncbigene:30364
LOC565849 GeneProduct ncbigene:565849
prkcha GeneProduct ncbigene:100144561
LOC100330801 GeneProduct ncbigene:100330801
wnt7bb GeneProduct ncbigene:100148840
wnt5b GeneProduct ncbigene:30105
zgc:194761 GeneProduct ncbigene:557384
ppp2r4 GeneProduct ncbigene:445031
lrp5 GeneProduct ncbigene:568518
ccnd2a GeneProduct ncbigene:799608


  1. Manipulation of self-renewal in human embryonic stem cells through a novel pharmacological GSK-3 inhibitor. Sato N, Brivanlou AH. Methods Mol Biol. 2006;331:115–28. PubMed Europe PMC Scholia
  2. Wnt/beta-catenin/CBP signaling maintains long-term murine embryonic stem cell pluripotency. Miyabayashi T, Teo JL, Yamamoto M, McMillan M, Nguyen C, Kahn M. Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5668–73. PubMed Europe PMC Scholia
  3. Tcf3 functions as a steady-state limiter of transcriptional programs of mouse embryonic stem cell self-renewal. Yi F, Pereira L, Merrill BJ. Stem Cells. 2008 Aug;26(8):1951–60. PubMed Europe PMC Scholia
  4. Esrrb activates Oct4 transcription and sustains self-renewal and pluripotency in embryonic stem cells. Zhang X, Zhang J, Wang T, Esteban MA, Pei D. J Biol Chem. 2008 Dec 19;283(51):35825–33. PubMed Europe PMC Scholia
  5. Glypican4 promotes cardiac specification and differentiation by attenuating canonical Wnt and Bmp signaling. Strate I, Tessadori F, Bakkers J. Development. 2015 May 15;142(10):1767–76. PubMed Europe PMC Scholia
  6. Both ciliary and non-ciliary functions of Foxj1a confer Wnt/β-catenin signaling in zebrafish left-right patterning. Zhu P, Xu X, Lin X. Biol Open. 2015 Oct 2;4(11):1376–86. PubMed Europe PMC Scholia