mRNA processing (WP123)

Caenorhabditis elegans

This process describes the conversion of precursor messenger RNA into mature messenger RNA (mRNA). The pre-mRNA molecule undergoes three main modifications. These modifications are 5' capping, 3' polyadenylation, and RNA splicing, which occur in the cell nucleus before the RNA is translated. 5' Capping: Capping of the pre-mRNA involves the addition of 7-methylguanosine (m7G) to the 5' end. The cap protects the 5' end of the primary RNA transcript from attack by ribonucleases that have specificity to the 3'5' phosphodiester bonds. 3' Processing: The pre-mRNA processing at the 3' end of the RNA molecule involves cleavage of its 3' end and then the addition of about 200 adenine residues to form a poly(A) tail. As the poly(A) tails is synthesised, it binds multiple copies of poly(A) binding protein, which protects the 3'end from ribonuclease digestion. Splicing: RNA splicing is the process by which introns, regions of RNA that do not code for protein, are removed from the pre-mRNA and the remaining exons connected to re-form a single continuous molecule. Description adapted from Wikipedia:


Nathan Salomonis , Kristina Hanspers , Daniela Digles , Friederike Ehrhart , Denise Slenter , Alex Pico , and Lars Willighagen


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Caenorhabditis elegans



Pathway Ontology

spliceosome pathway


Label Type Compact URI Comment
prp-8 GeneProduct ncbigene:176153
C27H5.3 GeneProduct ncbigene:174175
cpf-2 GeneProduct ncbigene:176742
M03C11.7 GeneProduct ncbigene:176461
prp-4 GeneProduct ncbigene:172443
prp-21 GeneProduct ncbigene:173440
Y76B12C.7 GeneProduct ncbigene:177003
F37E3.1 GeneProduct ncbigene:172345
rsp-6 GeneProduct ncbigene:177566
rsp-1 GeneProduct ncbigene:174748
prp-17 GeneProduct ncbigene:172999
F43G9.5 GeneProduct ncbigene:172657
snr-4 GeneProduct ncbigene:179639
snr-6 GeneProduct ncbigene:176668
F11A10.2 GeneProduct ncbigene:178147
him-1 GeneProduct ncbigene:172116
W03F9.10 GeneProduct ncbigene:178542
F56D2.6 GeneProduct ncbigene:175771
tag-172 GeneProduct ncbigene:3565068
hrp-2 GeneProduct ncbigene:173086
unc-75 GeneProduct ncbigene:182722
nxf-1 GeneProduct ncbigene:191736
F59A2.4 GeneProduct ncbigene:175442
sap-1 GeneProduct ncbigene:173767
Y48B6A.3 GeneProduct ncbigene:175071
rsp-2 GeneProduct ncbigene:174747
F09G2.4 GeneProduct ncbigene:179103
T08B2.5 GeneProduct ncbigene:172312
C25A1.4 GeneProduct ncbigene:172881
ama-1 GeneProduct ncbigene:177190
Y55F3BR.1 GeneProduct ncbigene:176913
rha-1 GeneProduct ncbigene:174417
snr-5 GeneProduct ncbigene:176103
F28C1.1 GeneProduct ncbigene:179705
T13H5.4 GeneProduct ncbigene:174364
rsp-3 GeneProduct ncbigene:176688 Antagonist splicing factor
T08A11.2 GeneProduct ncbigene:175549
F08G12.2 GeneProduct ncbigene:181319
rsp-4 GeneProduct ncbigene:173915
rsr-1 GeneProduct ncbigene:173013
etr-1 GeneProduct ncbigene:173401
ptb-1 GeneProduct ncbigene:174647
pab-3 GeneProduct ncbigene:172768
F58B3.7 GeneProduct ncbigene:178091
gut-2 GeneProduct ncbigene:179833
F43G6.5 GeneProduct ncbigene:174816
mog-4 GeneProduct ncbigene:175117
C44B7.2 GeneProduct ncbigene:3565706
gut-2 GeneProduct ncbigene:179833
bath-43 GeneProduct ncbigene:176422
eft-1 GeneProduct ncbigene:175851
tag-72 GeneProduct ncbigene:182875
rnp-3 GeneProduct ncbigene:177182
Y113G7B.17 GeneProduct ncbigene:180326
cel-1 GeneProduct ncbigene:172814
spt-5 GeneProduct ncbigene:178143
lsm-7 GeneProduct ncbigene:177988
uaf-1 GeneProduct ncbigene:175270
teg-1 GeneProduct ncbigene:176561
dcr-1 GeneProduct ncbigene:176138
rsp-7 GeneProduct ncbigene:174646 PMID: 14559993. SRrp86 is a unique member of the SR protein superfamily containing one RNA recognition motif and two serine-arginine (SR)-rich domains separated by an unusual glutamic acid-lysine (EK)-rich region. Previously, we showed that SRrp86 could regulate alternative splicing by both positively and negatively modulating the activity of other SR proteins and that the unique EK domain could inhibit both constitutive and alternative splicing. These functions were most consistent with the model in which SRrp86 functions by interacting with and thereby modulating the activity of target proteins. To identify the specific proteins that interact with SRrp86, we used a yeast two-hybrid library screen and immunoprecipitation coupled to mass spectrometry. We show that SRrp86 interacts with all of the core SR proteins, as well as a subset of other splicing regulatory proteins, including SAF-B, hnRNP G, YB-1, and p72. In contrast to previous results that showed activation of SRp20 by SRrp86, we now show that SAF-B, hnRNP G, and 9G8 all antagonize the activity of SRrp86. Overall, we conclude that not only does SRrp86 regulate SR protein activity but that it is, in turn, regulated by other splicing factors to control alternative splice site selection.
C36B1.5 GeneProduct ncbigene:183250
prp-17 GeneProduct ncbigene:172999
phf-5 GeneProduct ncbigene:3564851
mog-1 GeneProduct ncbigene:176409
dnj-30 GeneProduct ncbigene:171833
F11A10.8 GeneProduct ncbigene:178151
Y59A8B.6 GeneProduct ncbigene:180180
rnp-7 GeneProduct ncbigene:176002
mog-5 GeneProduct ncbigene:173920
Y55F3AM.3 GeneProduct ncbigene:176922
snr-1 GeneProduct ncbigene:178483
snr-7 GeneProduct ncbigene:171834
F47G9.1 GeneProduct ncbigene:179567
C36B1.5 GeneProduct ncbigene:183250
snr-3 GeneProduct ncbigene:174072
Y73B6BL.33 GeneProduct ncbigene:177398
Y67H2A.1 GeneProduct ncbigene:178285
tag-203 GeneProduct ncbigene:172406
prp-8 GeneProduct ncbigene:176153
suf-1 GeneProduct ncbigene:174380
cpf-1 GeneProduct ncbigene:174378
rnp-2 GeneProduct ncbigene:177181


  1. Pre-mRNA splicing and its regulation in Caenorhabditis elegans. Zahler AM. WormBook [Internet]. 2012; Available from: DOI Scholia