mRNA processing (WP1229)

Anopheles gambiae

This process describes the conversion of precursor messenger RNA into mature messenger RNA (mRNA). The pre-mRNA molecule undergoes three main modifications. These modifications are 5' capping, 3' polyadenylation, and RNA splicing, which occur in the cell nucleus before the RNA is translated. 5' Capping: Capping of the pre-mRNA involves the addition of 7-methylguanosine (m7G) to the 5' end. The cap protects the 5' end of the primary RNA transcript from attack by ribonucleases that have specificity to the 3'5' phosphodiester bonds. 3' Processing: The pre-mRNA processing at the 3' end of the RNA molecule involves cleavage of its 3' end and then the addition of about 200 adenine residues to form a poly(A) tail. As the poly(A) tails is synthesised, it binds multiple copies of poly(A) binding protein, which protects the 3'end from ribonuclease digestion. Splicing: RNA splicing is the process by which introns, regions of RNA that do not code for protein, are removed from the pre-mRNA and the remaining exons connected to re-form a single continuous molecule. Description adapted from Wikipedia:


Kristina Hanspers , Daniela Digles , Alex Pico , and Martina Summer-Kutmon


last edited

Discuss this pathway

Check for ongoing discussions or start your own.

Cited In

Are you planning to include this pathway in your next publication? See How to Cite and add a link here to your paper once it's online.


Anopheles gambiae



Pathway Ontology

spliceosome pathway


Label Type Compact URI Comment
GMP Metabolite hmdb:HMDB0001397
AgaP_AGAP002144 GeneProduct ncbigene:1269414
AgaP_AGAP007670 GeneProduct ncbigene:1269557
AgaP_AGAP009296 GeneProduct ncbigene:1280262
AgaP_AGAP012380 GeneProduct ncbigene:1280333
AgaP_AGAP005298 GeneProduct ncbigene:1270385
AgaP_AGAP005142 GeneProduct ncbigene:1274820
AgaP_AGAP007246 GeneProduct ncbigene:1269901
AgaP_AGAP011035 GeneProduct ncbigene:1269464
AgaP_AGAP003936 GeneProduct ncbigene:1278762
AgaP_AGAP004090 GeneProduct ncbigene:1270887
AgaP_AGAP001792 GeneProduct ncbigene:1281323 GenBank sequence: M10329
AgaP_AGAP009093 GeneProduct ncbigene:1280046
AgaP_AGAP001460 GeneProduct ncbigene:1281715
AgaP_AGAP002374 GeneProduct ncbigene:3290608
AgaP_AGAP009477 GeneProduct ncbigene:1271433
AgaP_AGAP003428 GeneProduct ncbigene:1272801
AgaP_AGAP009905 GeneProduct ncbigene:1279319
AgaP_AGAP011907 GeneProduct ncbigene:1280756
AgaP_AGAP001419 GeneProduct ncbigene:1281757
AgaP_AGAP005640 GeneProduct ncbigene:1276326
AgaP_AGAP006108 GeneProduct ncbigene:4576730
AgaP_AGAP005549 GeneProduct ncbigene:1276233
AgaP_AGAP008263 GeneProduct ncbigene:1277723
AgaP_AGAP009506 GeneProduct ncbigene:1271405
AgaP_AGAP008122 GeneProduct ncbigene:1277833
AgaP_AGAP004765 GeneProduct ncbigene:1278460
AgaP_AGAP005322 GeneProduct ncbigene:1276029 PMID: 12773558
AgaP_AGAP002956 GeneProduct ncbigene:1273007
AgaP_AGAP005021 GeneProduct ncbigene:4576284
AgaP_AGAP004610 GeneProduct ncbigene:1275923 PMID: 14729963
AgaP_AGAP010496 GeneProduct ncbigene:1275237 Antagonist splicing factor
AgaP_AGAP007701 GeneProduct ncbigene:1269532
AgaP_AGAP002658 GeneProduct ncbigene:1273301
AgaP_AGAP002895 GeneProduct ncbigene:1273072
AgaP_AGAP004592 GeneProduct ncbigene:1275942
AgaP_AGAP002193 GeneProduct ncbigene:1269364
AgaP_AGAP006240 GeneProduct ncbigene:1276901
AgaP_AGAP003794 GeneProduct ncbigene:1271533
AgaP_AGAP008577 GeneProduct ncbigene:1275439
AgaP_AGAP004682 GeneProduct ncbigene:1268691
AgaP_AGAP003019 GeneProduct ncbigene:1272933
AgaP_AGAP000399 GeneProduct ncbigene:1271847
AgaP_AGAP001224 GeneProduct ncbigene:1281946
AgaP_AGAP006668 GeneProduct ncbigene:4576768
AgaP_AGAP002614 GeneProduct ncbigene:1273354
AgaP_AGAP007810 GeneProduct ncbigene:1278148
AgaP_AGAP005117 GeneProduct ncbigene:1274794
AgaP_AGAP003462 GeneProduct ncbigene:1272833
AgaP_AGAP003635 GeneProduct ncbigene:1274299
AgaP_AGAP007325 GeneProduct ncbigene:1269848
AgaP_AGAP011328 GeneProduct ncbigene:1270609
AgaP_AGAP011494 GeneProduct ncbigene:1278378
AgaP_AGAP005136 GeneProduct ncbigene:1274814
AgaP_AGAP001064 GeneProduct ncbigene:1278678
AgaP_AGAP002474 GeneProduct ncbigene:1273485
AgaP_AGAP011340 GeneProduct ncbigene:1270599
AgaP_AGAP006171 GeneProduct ncbigene:1276838 PMID: 12773558
AgaP_AGAP007242 GeneProduct ncbigene:1269904
AgaP_AGAP001538 GeneProduct ncbigene:1281638
AgaP_AGAP004414 GeneProduct ncbigene:1274558
AgaP_AGAP008528 GeneProduct ncbigene:1277444
AgaP_AGAP002265 GeneProduct ncbigene:1269293 PMID: 14559993. SRrp86 is a unique member of the SR protein superfamily containing one RNA recognition motif and two serine-arginine (SR)-rich domains separated by an unusual glutamic acid-lysine (EK)-rich region. Previously, we showed that SRrp86 could regulate alternative splicing by both positively and negatively modulating the activity of other SR proteins and that the unique EK domain could inhibit both constitutive and alternative splicing. These functions were most consistent with the model in which SRrp86 functions by interacting with and thereby modulating the activity of target proteins. To identify the specific proteins that interact with SRrp86, we used a yeast two-hybrid library screen and immunoprecipitation coupled to mass spectrometry. We show that SRrp86 interacts with all of the core SR proteins, as well as a subset of other splicing regulatory proteins, including SAF-B, hnRNP G, YB-1, and p72. In contrast to previous results that showed activation of SRp20 by SRrp86, we now show that SAF-B, hnRNP G, and 9G8 all antagonize the activity of SRrp86. Overall, we conclude that not only does SRrp86 regulate SR protein activity but that it is, in turn, regulated by other splicing factors to control alternative splice site selection.
AgaP_AGAP006885 GeneProduct ncbigene:1270195
AgaP_AGAP000178 GeneProduct ncbigene:1272086
AgaP_AGAP002144 GeneProduct ncbigene:1269414
AgaP_AGAP004950 GeneProduct ncbigene:1275761
AgaP_AGAP002307 GeneProduct ncbigene:1273662
AgaP_AGAP010918 GeneProduct ncbigene:1271032
AgaP_AGAP003945 GeneProduct ncbigene:1278776
AgaP_AGAP002776 GeneProduct ncbigene:1273194
AgaP_AGAP012054 GeneProduct ncbigene:1280613
AgaP_AGAP002908 GeneProduct ncbigene:1273053
AgaP_AGAP008303 GeneProduct ncbigene:1277682
AgaP_AGAP006599 GeneProduct ncbigene:1277182
AgaP_AGAP005366 GeneProduct ncbigene:3289953
AgaP_AGAP003460 GeneProduct ncbigene:1272829
AgaP_AGAP008925 GeneProduct ncbigene:1279895
AgaP_AGAP002706 GeneProduct ncbigene:1273262
AgaP_AGAP005523 GeneProduct ncbigene:1276207
AgaP_AGAP002947 GeneProduct ncbigene:1273015
AgaP_AGAP006755 GeneProduct ncbigene:1270305
AgaP_AGAP006885 GeneProduct ncbigene:1270195
AgaP_AGAP008298 GeneProduct ncbigene:1277686
AgaP_AGAP001195 GeneProduct ncbigene:1281974
AgaP_AGAP008925 GeneProduct ncbigene:1279895
AgaP_AGAP007212 GeneProduct ncbigene:1269919
AgaP_AGAP000847 GeneProduct ncbigene:1277360
AgaP_AGAP005136 GeneProduct ncbigene:1274814
AgaP_AGAP008433 GeneProduct ncbigene:1277542
AgaP_AGAP011637 GeneProduct ncbigene:1280995
AgaP_AGAP001645 GeneProduct ncbigene:1281527
AgaP_AGAP009570 GeneProduct ncbigene:1278931 PMID: 14729963
AgaP_AGAP011149 GeneProduct ncbigene:1270778