Colanic acid building blocks biosynthesis (WP121)

Saccharomyces cerevisiae

Colanic acid (or M antigen) is a type of extracellular polysaccharide. It consists of a polyanionic heteropolysaccharide that contains a nonstoichiometric combination of sugars such as D-glucose, L-fucoses, D-galactose, and D-glucuronate, which are decorated with O-acetyl and pyruvate side chains. Before assembly, the sugars must be activated in the form of nucleotide sugars. The process of assembling the colanic acid polysaccharide repeat takes place on the membrane lipid through a series of glycosyl transferases on the cytoplasmic side of the inner membrane. Once the single repeat is assembled, it is flipped to the periplasmic side and polymerized by the Wzy-dependent pathway.


Meredith Braymer , Egon Willighagen , Anders Riutta , Denise Slenter , Eric Weitz , and Alex Pico


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Saccharomyces cerevisiae



Pathway Ontology

classic metabolic pathway


Label Type Compact URI Comment
UDP-D-glucose Metabolite wikidata:Q27102800
UTP Metabolite chebi:46398
NAD Metabolite chebi:13389
GDP-mannose Metabolite chebi:84880
NADP Metabolite chebi:44409
UDP-D-glucose Metabolite wikidata:Q27102800
H2O Metabolite chebi:15377
GDP-4-dehydro-6-deoxy-D-mannose Metabolite chebi:16955
UDP-galactose Metabolite wikidata:Q259015
UDP-D-glucuronate Metabolite chebi:17200
alpha-D-Galactose Metabolite chebi:28061
H+ Metabolite chebi:15378
Alpha-D-Galactose-1-Phosphate Metabolite wikidata:Q3082858
UDP-D-glucose Metabolite chebi:46229
H2O Metabolite chebi:15377
phosphate Metabolite cas:14265-44-2
guanosine diphosphate fucose Metabolite cas:15839-70-0
NADPH Metabolite cas:53-57-6
mannose-6-phosphate Metabolite chebi:43896
glucose-1-phosphate Metabolite chebi:16077
pyrophosphate Metabolite cas:2466-09-3
glucose-1-phosphate Metabolite chebi:16077
NADH Metabolite hmdb:HMDB0001487
mannose-1-phosphate Metabolite chebi:35374
GDP Metabolite cas:146-91-8
ATP Metabolite cas:1927-31-7
fructose-6-phosphate Metabolite pubchem.compound:15648788
ADP Metabolite cas:58-64-0
GAL10 GeneProduct sgd:S000000223
PMI40 GeneProduct sgd:S000000805
UGP1 GeneProduct sgd:S000001518
SEC53 GeneProduct sgd:S000001849
YHL012W GeneProduct sgd:S000001004
GAL7 GeneProduct sgd:S000000222
GAL1 GeneProduct sgd:S000000224
PSA1 GeneProduct ensembl:YDL055C