Cholesterol biosynthesis (WP103)

Mus musculus

Cholesterol is a waxy steroid metabolite found in the cell membranes and transported in the blood plasma of all animals. It is an essential structural component of mammalian cell membranes, where it is required to establish proper membrane permeability and fluidity. In addition, cholesterol is an important component for the manufacture of bile acids, steroid hormones, and several fat-soluble vitamins. Cholesterol is the principal sterol synthesized by animals, but small quantities are synthesized in other eukaryotes, such as plants and fungi. It is almost completely absent among prokaryotes, which include bacteria. Source: [[wikipedia:Cholesterol|Wikipedia]]


Michael Lieberman , Ned Mantei , Thomas Kelder , Christine Chichester , Egon Willighagen , Irene , Denise Slenter , and Eric Weitz


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Mus musculus



Pathway Ontology

cholesterol biosynthetic pathway


Label Type Compact Identifier Comment
(S)-2,3-Epoxysqualene Metabolite chebi:15441
Mevalonic acid Metabolite cas:150-97-0
farnesyl pyrophosphate Metabolite cas:13058-04-3
Lathosterol Metabolite cas:80-99-9
Lanosterin Metabolite cas:79-63-0
Mevalonic acid 5-pyrophosphate Metabolite cas:1492-08-6
7-Dehydrocholesterol Metabolite cas:434-16-2
isopentenyl pyrophosphate Metabolite cas:358-71-4
Geranyl-PP Metabolite cas:6699-20-3
Cholesterol Metabolite cas:57-88-5
Mevalonic acid-5P Metabolite cas:73566-35-5
Squalene Metabolite cas:111-02-4
Acetyl-CoA Metabolite hmdb:HMDB0001206
Dimethylallylpyrophosphate Metabolite cas:358-72-5
Nsdhl GeneProduct ncbigene:18194 NAD(P) dependent steroid dehydrogenase-like
Fdps GeneProduct ncbigene:110196
Hmgcs1 GeneProduct ncbigene:208715
Sc4mol GeneProduct ncbigene:66234
Sqle GeneProduct ncbigene:20775
Idi1 GeneProduct ncbigene:319554
Mvd GeneProduct ncbigene:192156
Cyp51 GeneProduct ncbigene:13121
Hmgcr GeneProduct ncbigene:15357
Dhcr7 GeneProduct ncbigene:13360
Pmvk GeneProduct ncbigene:68603
Fdps GeneProduct ncbigene:110196
Sc5d GeneProduct ncbigene:235293
Fdft1 GeneProduct ncbigene:14137
Lss GeneProduct ncbigene:16987
Mvk GeneProduct ncbigene:17855


  1. Geranylgeranyl diphosphate synthase catalyzing the single condensation between isopentenyl diphosphate and farnesyl diphosphate. Sagami H, Korenaga T, Ogura K. J Biochem. 1993 Jul;114(1):118–21. PubMed Europe PMC Scholia
  2. Cloning and characterization of ERG25, the Saccharomyces cerevisiae gene encoding C-4 sterol methyl oxidase. Bard M, Bruner DA, Pierson CA, Lees ND, Biermann B, Frye L, et al. Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):186–90. PubMed Europe PMC Scholia
  3. Characterization of yeast methyl sterol oxidase (ERG25) and identification of a human homologue. Li L, Kaplan J. J Biol Chem. 1996 Jul 12;271(28):16927–33. PubMed Europe PMC Scholia
  4. Structural and evolutionary studies on sterol 14-demethylase P450 (CYP51), the most conserved P450 monooxygenase: I. Structural analyses of the gene and multiple sizes of mRNA. Noshiro M, Aoyama Y, Kawamoto T, Gotoh O, Horiuchi T, Yoshida Y. J Biochem. 1997 Dec;122(6):1114–21. PubMed Europe PMC Scholia
  5. The gene mutated in bare patches and striated mice encodes a novel 3beta-hydroxysteroid dehydrogenase. Liu XY, Dangel AW, Kelley RI, Zhao W, Denny P, Botcherby M, et al. Nat Genet. 1999 Jun;22(2):182–7. PubMed Europe PMC Scholia
  6. Membrane-bound enzymes of cholesterol synthesis from lanosterol. Gaylor JL. Biochem Biophys Res Commun. 2002 Apr 19;292(5):1139–46. PubMed Europe PMC Scholia
  7. Localization of mammalian NAD(P)H steroid dehydrogenase-like protein on lipid droplets. Ohashi M, Mizushima N, Kabeya Y, Yoshimori T. J Biol Chem. 2003 Sep 19;278(38):36819–29. PubMed Europe PMC Scholia