mRNA processing (Anopheles gambiae)

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Rnmt (methyltransferase)exon 25intronU5mRNA Capping(1)exon 1PiU2 snRNPs65heterogeneous nuclear ribonucleoproteinsU6Additional FactorsCAUGSR protein kinasesYYYY5mRNA 3'-end processingexon 1Cleavage of mRNA at the 3'-endSR-kinasesexon 2CF IIexon 23Formation of the Spliceosomal A complex3hnRNPsFormation of pre-mRNPsYYYYAdditional Factors5SR-phosphatasespre-mRNA5mRNA polyadenylation355mRNA splice site selectionexon 1AGAGAmRNA splicing (major pathway)GUAGSerine/Argenine Rich Proteins(SR)YYYYInternal methylation of mRNACF IIASR-binding sitesCstF(3)Cap Bindingexon 1U4:U5:U6 tri-snRNP complexCF ICFIAAUAAA5(2b)U4/U6(2)RNAi pathwayRNA helicase3UGsnRNP Sm core complexright click on blue text for detailed informationA(2c)3splicing factor SF3BSR-proteins3Misc.5U4AGFormation of the Spliceosomal B complexCstFSR-proteins5Formation of the Spliceosomal A complexAGP-Poly(A)pb IIexon 1Internal methylation of mRNALariat Formation and 5'-Splice Site CleavageRngtt (capping enzyme)U2AF5 G capexon 2U5U2 snRNPFormation of pre-mRNPsCPSFFormation of the Spliceosomal E complexSR-proteinsKinases Phosphorylating RS domains?U1 snRNP3Formation of the Spliceosomal C complexRNA helicase ACPSFMethylation of mRNA3SR-binding sites3mRNA capping5YYYYYYYYU2 snRNPNote: New participating members shown at each stepPoly(A)-binding protein IIRegulation ofAlternative SplicingSF3b14bhnRNP regulatorsCleavage at the 3'-Splice-Site and Exon LigationSR protein phosphatasessplicing factor SF3AU5 snRNPPutative Alternative SplicingRegulators35Accessory ProteinsPoly(A) polSR-repressor proteinsexon 2Polr2aU2U1 snRNPU4 snRNPProcessing of Intron-Containing Pre-mRNA3Poly(A) polymerase alpha(2a)degradedsnRNP Sm core complexsplicesome associated helicasesYYYYU6 snRNPAdditional SR proteinsAPrimary RNA transcriptexon 2AFormation of the Spliceosomal E complexAAAAAAAAiPoU1Formation of the active Spliceosomal C complex5Formation of the Spliceosomal B complex3snRNP Sm core complexAGAdditional FactorsPU2AFexon 1Spt5AgaP_AGAP002658AgaP_AGAP005142AgaP_AGAP004610SSFA1DICER1AgaP_AGAP006108AgaP_AGAP003936AgaP_AGAP007242U5-116KDAgaP_AGAP007325AgaP_AGAP008528ATPAgaP_AGAP002265AgaP_AGAP003460HNRPA2B1AgaP_AGAP002956AgaP_AGAP005366AgaP_AGAP005549AgaP_AGAP006599RBMXU4 snRNAAgaP_AGAP011035AgaP_AGAP001419HNRPA3P1AgaP_AGAP006885AgaP_AGAP003019RNU2AgaP_AGAP003794AgaP_AGAP000178AgaP_AGAP011328CLK1NCBP2SFRS2SF3A3AgaP_AGAP003635SFRS4FUSIP1AgaP_AGAP008433SFRS10CPSF4AgaP_AGAP008298SFRS5NONOAgaP_AGAP011637SSFA1AgaP_AGAP003945AgaP_AGAP008925HNRPCAgaP_AGAP006755GMPAgaP_AGAP006171AgaP_AGAP002144HNRPH2AgaP_AGAP001195SRPK2AgaP_AGAP006240AgaP_AGAP002706AgaP_AGAP006885AgaP_AGAP009905SFRS14SSFA1AgaP_AGAP002776AgaP_AGAP006668AgaP_AGAP008122SFRS9AgaP_AGAP001645AgaP_AGAP004090AgaP_AGAP005117AgaP_AGAP004950AgaP_AGAP010496AgaP_AGAP007810AgaP_AGAP002474U5 snRNAAgaP_AGAP007246AgaP_AGAP007670AgaP_AGAP001538CLK4AgaP_AGAP011149AgaP_AGAP011494AgaP_AGAP012054PSKH1AgaP_AGAP004414AgaP_AGAP011907AgaP_AGAP008925AgaP_AGAP005523AgaP_AGAP009570AgaP_AGAP005298U1 snRNAAgaP_AGAP003462AgaP_AGAP002947AgaP_AGAP001792AgaP_AGAP004682SNRPNAgaP_AGAP002614PRPF4BAgaP_AGAP002307CUGBP1DDX20AgaP_AGAP001064AgaP_AGAP005021PAPOLASF4AgaP_AGAP004592AgaP_AGAP009506PCBP2AgaP_AGAP002193FUSIP1AgaP_AGAP008263PTBP2AgaP_AGAP002374AgaP_AGAP007212SNRPB2AgaP_AGAP002895AgaP_AGAP009477AgaP_AGAP000399HNRPUAgaP_AGAP010918AgaP_AGAP008577AgaP_AGAP009296AgaP_AGAP012380AgaP_AGAP005136AgaP_AGAP003428HNRPH1AgaP_AGAP004765HRMT1L1HNRPKAgaP_AGAP005322AgaP_AGAP000847AgaP_AGAP002908AgaP_AGAP011340AgaP_AGAP001224CLK2SNRPEAgaP_AGAP002144AgaP_AGAP008303AgaP_AGAP005640AgaP_AGAP001460SNRPNAgaP_AGAP005136AgaP_AGAP007701AgaP_AGAP009093


Description

This process describes the conversion of precursor messenger RNA into mature messenger RNA (mRNA). The pre-mRNA molecule undergoes three main modifications. These modifications are 5' capping, 3' polyadenylation, and RNA splicing, which occur in the cell nucleus before the RNA is translated.

5' Capping: Capping of the pre-mRNA involves the addition of 7-methylguanosine (m7G) to the 5' end. The cap protects the 5' end of the primary RNA transcript from attack by ribonucleases that have specificity to the 3'5' phosphodiester bonds.

3' Processing: The pre-mRNA processing at the 3' end of the RNA molecule involves cleavage of its 3' end and then the addition of about 200 adenine residues to form a poly(A) tail. As the poly(A) tails is synthesised, it binds multiple copies of poly(A) binding protein, which protects the 3'end from ribonuclease digestion.

Splicing: RNA splicing is the process by which introns, regions of RNA that do not code for protein, are removed from the pre-mRNA and the remaining exons connected to re-form a single continuous molecule.

Description adapted from Wikipedia: http://en.wikipedia.org/wiki/Post-transcriptional_modification

Comments

GenMAPP notes 
Gene lists from obtained from InterPro 9-23-2003: Mm RNA-binding region RNP-1 (RNA recognition motif)
GenMAPP remarks 
Adapted from http://www.reactome.org.
HomologyConvert 
This pathway was inferred from Homo sapiens pathway WP411(r30272) with a 65% conversion rate.

Quality Tags

Ontology Terms

 

Bibliography

No bibliography


History

View all...
CompareRevisionActionTimeUserComment
105892view11:21, 16 August 2019MkutmonHMDB identifier unification
94573view17:53, 28 September 2017AlexanderPicoremoved nested group
71445view19:12, 17 October 2013MaintBotAutomated update of data sources
68444view19:18, 5 July 2013MaintBotUpdated to 2013 gpml schema
67494view11:09, 26 June 2013DdiglesOntology Term : 'spliceosome pathway' added !
40476view18:47, 1 March 2011MaintBotRemoved redundant pathway information and comments
35234view19:22, 11 February 2010KhanspersPeriodical save, work in progress
35233view19:10, 11 February 2010KhanspersModified description
33733view23:44, 8 December 2009MaintBotAutomatic update of empty xrefs
32285view13:09, 15 August 2009MaintBotFixed text labels
31836view12:28, 14 August 2009MaintBotFixed group labels
31313view20:14, 13 August 2009MaintBotFixed text labels
30798view23:44, 29 July 2009MaintBotNew pathway

External references

DataNodes

View all...
NameTypeDatabase referenceComment
ATPMetabolite
AgaP_AGAP000178GeneProduct1272086 (Entrez Gene)
AgaP_AGAP000399GeneProduct1271847 (Entrez Gene)
AgaP_AGAP000847GeneProduct1277360 (Entrez Gene)
AgaP_AGAP001064GeneProduct1278678 (Entrez Gene)
AgaP_AGAP001195GeneProduct1281974 (Entrez Gene)
AgaP_AGAP001224GeneProduct1281946 (Entrez Gene)
AgaP_AGAP001419GeneProduct1281757 (Entrez Gene)
AgaP_AGAP001460GeneProduct1281715 (Entrez Gene)
AgaP_AGAP001538GeneProduct1281638 (Entrez Gene)
AgaP_AGAP001645GeneProduct1281527 (Entrez Gene)
AgaP_AGAP001792GeneProduct1281323 (Entrez Gene) GenBank sequence: M10329
AgaP_AGAP002144GeneProduct1269414 (Entrez Gene)
AgaP_AGAP002193GeneProduct1269364 (Entrez Gene)
AgaP_AGAP002265GeneProduct1269293 (Entrez Gene) PMID: 14559993. SRrp86 is a unique member of the SR protein superfamily containing one RNA recognition motif and two serine-arginine (SR)-rich domains separated by an unusual glutamic acid-lysine (EK)-rich region. Previously, we showed that SRrp86 could regulate alternative splicing by both positively and negatively modulating the activity of other SR proteins and that the unique EK domain could inhibit both constitutive and alternative splicing. These functions were most consistent with the model in which SRrp86 functions by interacting with and thereby modulating the activity of target proteins. To identify the specific proteins that interact with SRrp86, we used a yeast two-hybrid library screen and immunoprecipitation coupled to mass spectrometry. We show that SRrp86 interacts with all of the core SR proteins, as well as a subset of other splicing regulatory proteins, including SAF-B, hnRNP G, YB-1, and p72. In contrast to previous results that showed activation of SRp20 by SRrp86, we now show that SAF-B, hnRNP G, and 9G8 all antagonize the activity of SRrp86. Overall, we conclude that not only does SRrp86 regulate SR protein activity but that it is, in turn, regulated by other splicing factors to control alternative splice site selection.
AgaP_AGAP002307GeneProduct1273662 (Entrez Gene)
AgaP_AGAP002374GeneProduct3290608 (Entrez Gene)
AgaP_AGAP002474GeneProduct1273485 (Entrez Gene)
AgaP_AGAP002614GeneProduct1273354 (Entrez Gene)
AgaP_AGAP002658GeneProduct1273301 (Entrez Gene)
AgaP_AGAP002706GeneProduct1273262 (Entrez Gene)
AgaP_AGAP002776GeneProduct1273194 (Entrez Gene)
AgaP_AGAP002895GeneProduct1273072 (Entrez Gene)
AgaP_AGAP002908GeneProduct1273053 (Entrez Gene)
AgaP_AGAP002947GeneProduct1273015 (Entrez Gene)
AgaP_AGAP002956GeneProduct1273007 (Entrez Gene)
AgaP_AGAP003019GeneProduct1272933 (Entrez Gene)
AgaP_AGAP003428GeneProduct1272801 (Entrez Gene)
AgaP_AGAP003460GeneProduct1272829 (Entrez Gene)
AgaP_AGAP003462GeneProduct1272833 (Entrez Gene)
AgaP_AGAP003635GeneProduct1274299 (Entrez Gene)
AgaP_AGAP003794GeneProduct1271533 (Entrez Gene)
AgaP_AGAP003936GeneProduct1278762 (Entrez Gene)
AgaP_AGAP003945GeneProduct1278776 (Entrez Gene)
AgaP_AGAP004090GeneProduct1270887 (Entrez Gene)
AgaP_AGAP004414GeneProduct1274558 (Entrez Gene)
AgaP_AGAP004592GeneProduct1275942 (Entrez Gene)
AgaP_AGAP004610GeneProduct1275923 (Entrez Gene) PMID: 14729963
AgaP_AGAP004682GeneProduct1268691 (Entrez Gene)
AgaP_AGAP004765GeneProduct1278460 (Entrez Gene)
AgaP_AGAP004950GeneProduct1275761 (Entrez Gene)
AgaP_AGAP005021GeneProduct4576284 (Entrez Gene)
AgaP_AGAP005117GeneProduct1274794 (Entrez Gene)
AgaP_AGAP005136GeneProduct1274814 (Entrez Gene)
AgaP_AGAP005142GeneProduct1274820 (Entrez Gene)
AgaP_AGAP005298GeneProduct1270385 (Entrez Gene)
AgaP_AGAP005322GeneProduct1276029 (Entrez Gene) PMID: 12773558
AgaP_AGAP005366GeneProduct3289953 (Entrez Gene)
AgaP_AGAP005523GeneProduct1276207 (Entrez Gene)
AgaP_AGAP005549GeneProduct1276233 (Entrez Gene)
AgaP_AGAP005640GeneProduct1276326 (Entrez Gene)
AgaP_AGAP006108GeneProduct4576730 (Entrez Gene)
AgaP_AGAP006171GeneProduct1276838 (Entrez Gene) PMID: 12773558
AgaP_AGAP006240GeneProduct1276901 (Entrez Gene)
AgaP_AGAP006599GeneProduct1277182 (Entrez Gene)
AgaP_AGAP006668GeneProduct4576768 (Entrez Gene)
AgaP_AGAP006755GeneProduct1270305 (Entrez Gene)
AgaP_AGAP006885GeneProduct1270195 (Entrez Gene)
AgaP_AGAP007212GeneProduct1269919 (Entrez Gene)
AgaP_AGAP007242GeneProduct1269904 (Entrez Gene)
AgaP_AGAP007246GeneProduct1269901 (Entrez Gene)
AgaP_AGAP007325GeneProduct1269848 (Entrez Gene)
AgaP_AGAP007670GeneProduct1269557 (Entrez Gene)
AgaP_AGAP007701GeneProduct1269532 (Entrez Gene)
AgaP_AGAP007810GeneProduct1278148 (Entrez Gene)
AgaP_AGAP008122GeneProduct1277833 (Entrez Gene)
AgaP_AGAP008263GeneProduct1277723 (Entrez Gene)
AgaP_AGAP008298GeneProduct1277686 (Entrez Gene)
AgaP_AGAP008303GeneProduct1277682 (Entrez Gene)
AgaP_AGAP008433GeneProduct1277542 (Entrez Gene)
AgaP_AGAP008528GeneProduct1277444 (Entrez Gene)
AgaP_AGAP008577GeneProduct1275439 (Entrez Gene)
AgaP_AGAP008925GeneProduct1279895 (Entrez Gene)
AgaP_AGAP009093GeneProduct1280046 (Entrez Gene)
AgaP_AGAP009296GeneProduct1280262 (Entrez Gene)
AgaP_AGAP009477GeneProduct1271433 (Entrez Gene)
AgaP_AGAP009506GeneProduct1271405 (Entrez Gene)
AgaP_AGAP009570GeneProduct1278931 (Entrez Gene) PMID: 14729963
AgaP_AGAP009905GeneProduct1279319 (Entrez Gene)
AgaP_AGAP010496GeneProduct1275237 (Entrez Gene) Antagonist splicing factor
AgaP_AGAP010918GeneProduct1271032 (Entrez Gene)
AgaP_AGAP011035GeneProduct1269464 (Entrez Gene)
AgaP_AGAP011149GeneProduct1270778 (Entrez Gene)
AgaP_AGAP011328GeneProduct1270609 (Entrez Gene)
AgaP_AGAP011340GeneProduct1270599 (Entrez Gene)
AgaP_AGAP011494GeneProduct1278378 (Entrez Gene)
AgaP_AGAP011637GeneProduct1280995 (Entrez Gene)
AgaP_AGAP011907GeneProduct1280756 (Entrez Gene)
AgaP_AGAP012054GeneProduct1280613 (Entrez Gene)
AgaP_AGAP012380GeneProduct1280333 (Entrez Gene)
CLK1GeneProductPMID: 15010457: Inhibitor John C. Bell, PhD

Professor, Depts. of Medicine and Biochemistry, Microbiology & Immunology University of Ottawa Career Scientist, Ottawa Regional Cancer Centre Associate Scientist, The Ottawa Hospital Research Institute Telephone: 613-737-7700 ext 6893 Fax: 613-247-3524 Email: John.Bell@orcc.on.ca The control of pre-mRNA splicing by the Clk kinase family

We are studying a family of kinases which we believe provide an interface between intracellular signaling networks and the post-transcriptional mechanism of mRNA splicing. We are performing a structure:function analysis of the three Clk family members to identify domains in the three proteins which are involved in regulating splicing. Using homologous recombination we are generating null strains of mice which are lacking one, two or all three Clk genes. The Clk kinases all possess dual specificity kinase activity and yeast expression systems are being used to produce large amounts of the kinase to perform a detailed analysis of the sites of serine, threonine and tyrosine autophosphorylation within the kinase.
CLK2GeneProduct
CLK4GeneProduct
CPSF4GeneProduct
CUGBP1GeneProduct
DDX20GeneProduct
DICER1GeneProduct
FUSIP1GeneProductFUSI MOUSE PMID: 11684676
GMPMetabolite
HNRPA2B1GeneProduct
HNRPA3P1GeneProduct
HNRPCGeneProduct
HNRPH1GeneProduct
HNRPH2GeneProduct
HNRPKGeneProduct
HNRPUGeneProduct
HRMT1L1GeneProduct
NCBP2GeneProduct
NONOGeneProduct
PAPOLAGeneProduct
PCBP2GeneProduct
PRPF4BGeneProduct
PSKH1GeneProductPMID: 12466556
PTBP2GeneProduct
RBMXGeneProduct
RNU2GeneProductGenBank sequence: K00027
SF3A3GeneProduct
SF4GeneProductinferred from: SURP 2, G patch protein, UniProt:O60378
SFRS10GeneProduct
SFRS14GeneProduct
SFRS2GeneProduct
SFRS4GeneProduct
SFRS5GeneProduct
SFRS9GeneProductPMID: 12738786, PMID: 12024014 (associated with hnRNP A1 splicing)
SNRPB2GeneProduct
SNRPEGeneProduct
SNRPNGeneProduct
SRPK2GeneProductPMID: 12773558
SSFA1GeneProduct
U1 snRNAGeneProductGenBank sequence: J00645
U4 snRNAGeneProductGenBank sequence: M10328, M18004
U5 snRNAGeneProductGenBank sequence: AB021173
U5-116KDGeneProduct

Annotated Interactions

No annotated interactions
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