mRNA processing (Anopheles gambiae)

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mRNA CappingU4/U6exon 1GUFormation of the Spliceosomal B complexProcessing of Intron-Containing Pre-mRNA(2)exon 2P-SF3b14b35Formation of pre-mRNPsU2RNA helicaseCPSFU6Additional SR proteinsAGexon 1Cleavage of mRNA at the 3'-endYYYYmRNA polyadenylationKinases Phosphorylating RS domains?intronPoly(A)pb IIYYYYUGU2 snRNP55 G capFormation of the Spliceosomal E complexSR-proteinsAG3SR-proteinsPrimary RNA transcriptSR-binding sitesCPSFexon 1exon 2Polr2ahnRNP regulatorsFormation of the Spliceosomal B complex553pre-mRNAA5mRNA cappingAGmRNA splicing (major pathway)Poly(A) polymerase alpha3SR-binding sitesCFImRNA 3'-end processingMethylation of mRNAexon 2YYYYYYYYCF IICAexon 2(2c)U5(2a)3U2 snRNPAAAAAAAexon 1U4:U5:U6 tri-snRNP complex5YYYY(3)5U2 snRNPsAdditional Factorsexon 1Rngtt (capping enzyme)AU1AFormation of the active Spliceosomal C complexright click on blue text for detailed informationRegulation ofAlternative Splicing3Formation of the Spliceosomal E complex5degradedPoly(A) polexon 2Formation of pre-mRNPsCleavage at the 3'-Splice-Site and Exon LigationSR-kinases653ANote: New participating members shown at each step3Internal methylation of mRNAsplicesome associated helicasesFormation of the Spliceosomal A complexSR protein phosphatasesP3U5Poly(A)-binding protein IIU2AFCstFheterogeneous nuclear ribonucleoproteinsAAUAAAU1 snRNPAInternal methylation of mRNA3AGAGsnRNP Sm core complexUGCF IIAdditional FactorsSR-proteinsRNAi pathway(1)SR-repressor proteinssplicing factor SF3BsnRNP Sm core complexAccessory ProteinsRNA helicase AmRNA splice site selection5Additional FactorsCstFSpt5U4U2AFU6 snRNPiPo5CF I(2b)SR protein kinasesFormation of the Spliceosomal A complexMisc.AGU5 snRNPPutative Alternative SplicingRegulatorsYYYYhnRNPsA3snRNP Sm core complexPi5Cap BindingFormation of the Spliceosomal C complexLariat Formation and 5'-Splice Site Cleavage3U4 snRNPexon 1U1 snRNP5splicing factor SF3ASR-phosphatasesSerine/Argenine Rich Proteins(SR)exon 2Rnmt (methyltransferase)AgaP_AGAP002706AgaP_AGAP001195HNRPUU4 snRNADDX20AgaP_AGAP006599SNRPECPSF4U5 snRNASFRS5AgaP_AGAP008925AgaP_AGAP002614AgaP_AGAP005142AgaP_AGAP006668AgaP_AGAP005322AgaP_AGAP000847CLK1AgaP_AGAP012380U1 snRNAGMPAgaP_AGAP000178AgaP_AGAP001460RBMXAgaP_AGAP003019AgaP_AGAP007701AgaP_AGAP001538PSKH1AgaP_AGAP006240AgaP_AGAP011035CLK2AgaP_AGAP002895AgaP_AGAP007325AgaP_AGAP007670AgaP_AGAP003794SNRPB2U5-116KDAgaP_AGAP004950AgaP_AGAP007810AgaP_AGAP005640AgaP_AGAP011907AgaP_AGAP005366AgaP_AGAP004090AgaP_AGAP005523SSFA1AgaP_AGAP001792SNRPNHNRPKAgaP_AGAP011637ATPAgaP_AGAP009570AgaP_AGAP005136AgaP_AGAP001419NCBP2AgaP_AGAP006171AgaP_AGAP008528AgaP_AGAP002776AgaP_AGAP009905AgaP_AGAP002144AgaP_AGAP001064SSFA1AgaP_AGAP004414HNRPCSNRPNAgaP_AGAP005136SF4AgaP_AGAP003428SFRS9NONOHNRPA2B1SFRS2AgaP_AGAP005021AgaP_AGAP010918SSFA1AgaP_AGAP003936AgaP_AGAP011328AgaP_AGAP005549AgaP_AGAP002908AgaP_AGAP007212AgaP_AGAP006108FUSIP1AgaP_AGAP002307AgaP_AGAP001224SFRS4AgaP_AGAP005298AgaP_AGAP002947AgaP_AGAP004610AgaP_AGAP006885RNU2PCBP2AgaP_AGAP002374AgaP_AGAP011494AgaP_AGAP006755AgaP_AGAP008263AgaP_AGAP008303AgaP_AGAP001645AgaP_AGAP002193HNRPA3P1AgaP_AGAP008433AgaP_AGAP011149AgaP_AGAP002265AgaP_AGAP003460AgaP_AGAP004765AgaP_AGAP008577SFRS10AgaP_AGAP008298DICER1AgaP_AGAP009093AgaP_AGAP007246AgaP_AGAP009296AgaP_AGAP004682PRPF4BSF3A3AgaP_AGAP000399PTBP2HNRPH1AgaP_AGAP003635AgaP_AGAP005117AgaP_AGAP007242AgaP_AGAP002956SFRS14AgaP_AGAP006885AgaP_AGAP009477HNRPH2HRMT1L1AgaP_AGAP003462AgaP_AGAP012054SRPK2CLK4AgaP_AGAP008925AgaP_AGAP002474AgaP_AGAP011340AgaP_AGAP008122AgaP_AGAP002658AgaP_AGAP002144AgaP_AGAP004592AgaP_AGAP009506AgaP_AGAP003945FUSIP1AgaP_AGAP010496PAPOLACUGBP1


Description

This process describes the conversion of precursor messenger RNA into mature messenger RNA (mRNA). The pre-mRNA molecule undergoes three main modifications. These modifications are 5' capping, 3' polyadenylation, and RNA splicing, which occur in the cell nucleus before the RNA is translated.

5' Capping: Capping of the pre-mRNA involves the addition of 7-methylguanosine (m7G) to the 5' end. The cap protects the 5' end of the primary RNA transcript from attack by ribonucleases that have specificity to the 3'5' phosphodiester bonds.

3' Processing: The pre-mRNA processing at the 3' end of the RNA molecule involves cleavage of its 3' end and then the addition of about 200 adenine residues to form a poly(A) tail. As the poly(A) tails is synthesised, it binds multiple copies of poly(A) binding protein, which protects the 3'end from ribonuclease digestion.

Splicing: RNA splicing is the process by which introns, regions of RNA that do not code for protein, are removed from the pre-mRNA and the remaining exons connected to re-form a single continuous molecule.

Description adapted from Wikipedia: http://en.wikipedia.org/wiki/Post-transcriptional_modification

Comments

GenMAPP notes 
Gene lists from obtained from InterPro 9-23-2003: Mm RNA-binding region RNP-1 (RNA recognition motif)
GenMAPP remarks 
Adapted from http://www.reactome.org.
HomologyConvert 
This pathway was inferred from Homo sapiens pathway WP411(r30272) with a 65% conversion rate.

Quality Tags

Ontology Terms

 

Bibliography

No bibliography


History

View all...
CompareRevisionActionTimeUserComment
105892view11:21, 16 August 2019MkutmonHMDB identifier unification
94573view17:53, 28 September 2017AlexanderPicoremoved nested group
71445view19:12, 17 October 2013MaintBotAutomated update of data sources
68444view19:18, 5 July 2013MaintBotUpdated to 2013 gpml schema
67494view11:09, 26 June 2013DdiglesOntology Term : 'spliceosome pathway' added !
40476view18:47, 1 March 2011MaintBotRemoved redundant pathway information and comments
35234view19:22, 11 February 2010KhanspersPeriodical save, work in progress
35233view19:10, 11 February 2010KhanspersModified description
33733view23:44, 8 December 2009MaintBotAutomatic update of empty xrefs
32285view13:09, 15 August 2009MaintBotFixed text labels
31836view12:28, 14 August 2009MaintBotFixed group labels
31313view20:14, 13 August 2009MaintBotFixed text labels
30798view23:44, 29 July 2009MaintBotNew pathway

External references

DataNodes

View all...
NameTypeDatabase referenceComment
ATPMetabolite
AgaP_AGAP000178GeneProduct1272086 (Entrez Gene)
AgaP_AGAP000399GeneProduct1271847 (Entrez Gene)
AgaP_AGAP000847GeneProduct1277360 (Entrez Gene)
AgaP_AGAP001064GeneProduct1278678 (Entrez Gene)
AgaP_AGAP001195GeneProduct1281974 (Entrez Gene)
AgaP_AGAP001224GeneProduct1281946 (Entrez Gene)
AgaP_AGAP001419GeneProduct1281757 (Entrez Gene)
AgaP_AGAP001460GeneProduct1281715 (Entrez Gene)
AgaP_AGAP001538GeneProduct1281638 (Entrez Gene)
AgaP_AGAP001645GeneProduct1281527 (Entrez Gene)
AgaP_AGAP001792GeneProduct1281323 (Entrez Gene) GenBank sequence: M10329
AgaP_AGAP002144GeneProduct1269414 (Entrez Gene)
AgaP_AGAP002193GeneProduct1269364 (Entrez Gene)
AgaP_AGAP002265GeneProduct1269293 (Entrez Gene) PMID: 14559993. SRrp86 is a unique member of the SR protein superfamily containing one RNA recognition motif and two serine-arginine (SR)-rich domains separated by an unusual glutamic acid-lysine (EK)-rich region. Previously, we showed that SRrp86 could regulate alternative splicing by both positively and negatively modulating the activity of other SR proteins and that the unique EK domain could inhibit both constitutive and alternative splicing. These functions were most consistent with the model in which SRrp86 functions by interacting with and thereby modulating the activity of target proteins. To identify the specific proteins that interact with SRrp86, we used a yeast two-hybrid library screen and immunoprecipitation coupled to mass spectrometry. We show that SRrp86 interacts with all of the core SR proteins, as well as a subset of other splicing regulatory proteins, including SAF-B, hnRNP G, YB-1, and p72. In contrast to previous results that showed activation of SRp20 by SRrp86, we now show that SAF-B, hnRNP G, and 9G8 all antagonize the activity of SRrp86. Overall, we conclude that not only does SRrp86 regulate SR protein activity but that it is, in turn, regulated by other splicing factors to control alternative splice site selection.
AgaP_AGAP002307GeneProduct1273662 (Entrez Gene)
AgaP_AGAP002374GeneProduct3290608 (Entrez Gene)
AgaP_AGAP002474GeneProduct1273485 (Entrez Gene)
AgaP_AGAP002614GeneProduct1273354 (Entrez Gene)
AgaP_AGAP002658GeneProduct1273301 (Entrez Gene)
AgaP_AGAP002706GeneProduct1273262 (Entrez Gene)
AgaP_AGAP002776GeneProduct1273194 (Entrez Gene)
AgaP_AGAP002895GeneProduct1273072 (Entrez Gene)
AgaP_AGAP002908GeneProduct1273053 (Entrez Gene)
AgaP_AGAP002947GeneProduct1273015 (Entrez Gene)
AgaP_AGAP002956GeneProduct1273007 (Entrez Gene)
AgaP_AGAP003019GeneProduct1272933 (Entrez Gene)
AgaP_AGAP003428GeneProduct1272801 (Entrez Gene)
AgaP_AGAP003460GeneProduct1272829 (Entrez Gene)
AgaP_AGAP003462GeneProduct1272833 (Entrez Gene)
AgaP_AGAP003635GeneProduct1274299 (Entrez Gene)
AgaP_AGAP003794GeneProduct1271533 (Entrez Gene)
AgaP_AGAP003936GeneProduct1278762 (Entrez Gene)
AgaP_AGAP003945GeneProduct1278776 (Entrez Gene)
AgaP_AGAP004090GeneProduct1270887 (Entrez Gene)
AgaP_AGAP004414GeneProduct1274558 (Entrez Gene)
AgaP_AGAP004592GeneProduct1275942 (Entrez Gene)
AgaP_AGAP004610GeneProduct1275923 (Entrez Gene) PMID: 14729963
AgaP_AGAP004682GeneProduct1268691 (Entrez Gene)
AgaP_AGAP004765GeneProduct1278460 (Entrez Gene)
AgaP_AGAP004950GeneProduct1275761 (Entrez Gene)
AgaP_AGAP005021GeneProduct4576284 (Entrez Gene)
AgaP_AGAP005117GeneProduct1274794 (Entrez Gene)
AgaP_AGAP005136GeneProduct1274814 (Entrez Gene)
AgaP_AGAP005142GeneProduct1274820 (Entrez Gene)
AgaP_AGAP005298GeneProduct1270385 (Entrez Gene)
AgaP_AGAP005322GeneProduct1276029 (Entrez Gene) PMID: 12773558
AgaP_AGAP005366GeneProduct3289953 (Entrez Gene)
AgaP_AGAP005523GeneProduct1276207 (Entrez Gene)
AgaP_AGAP005549GeneProduct1276233 (Entrez Gene)
AgaP_AGAP005640GeneProduct1276326 (Entrez Gene)
AgaP_AGAP006108GeneProduct4576730 (Entrez Gene)
AgaP_AGAP006171GeneProduct1276838 (Entrez Gene) PMID: 12773558
AgaP_AGAP006240GeneProduct1276901 (Entrez Gene)
AgaP_AGAP006599GeneProduct1277182 (Entrez Gene)
AgaP_AGAP006668GeneProduct4576768 (Entrez Gene)
AgaP_AGAP006755GeneProduct1270305 (Entrez Gene)
AgaP_AGAP006885GeneProduct1270195 (Entrez Gene)
AgaP_AGAP007212GeneProduct1269919 (Entrez Gene)
AgaP_AGAP007242GeneProduct1269904 (Entrez Gene)
AgaP_AGAP007246GeneProduct1269901 (Entrez Gene)
AgaP_AGAP007325GeneProduct1269848 (Entrez Gene)
AgaP_AGAP007670GeneProduct1269557 (Entrez Gene)
AgaP_AGAP007701GeneProduct1269532 (Entrez Gene)
AgaP_AGAP007810GeneProduct1278148 (Entrez Gene)
AgaP_AGAP008122GeneProduct1277833 (Entrez Gene)
AgaP_AGAP008263GeneProduct1277723 (Entrez Gene)
AgaP_AGAP008298GeneProduct1277686 (Entrez Gene)
AgaP_AGAP008303GeneProduct1277682 (Entrez Gene)
AgaP_AGAP008433GeneProduct1277542 (Entrez Gene)
AgaP_AGAP008528GeneProduct1277444 (Entrez Gene)
AgaP_AGAP008577GeneProduct1275439 (Entrez Gene)
AgaP_AGAP008925GeneProduct1279895 (Entrez Gene)
AgaP_AGAP009093GeneProduct1280046 (Entrez Gene)
AgaP_AGAP009296GeneProduct1280262 (Entrez Gene)
AgaP_AGAP009477GeneProduct1271433 (Entrez Gene)
AgaP_AGAP009506GeneProduct1271405 (Entrez Gene)
AgaP_AGAP009570GeneProduct1278931 (Entrez Gene) PMID: 14729963
AgaP_AGAP009905GeneProduct1279319 (Entrez Gene)
AgaP_AGAP010496GeneProduct1275237 (Entrez Gene) Antagonist splicing factor
AgaP_AGAP010918GeneProduct1271032 (Entrez Gene)
AgaP_AGAP011035GeneProduct1269464 (Entrez Gene)
AgaP_AGAP011149GeneProduct1270778 (Entrez Gene)
AgaP_AGAP011328GeneProduct1270609 (Entrez Gene)
AgaP_AGAP011340GeneProduct1270599 (Entrez Gene)
AgaP_AGAP011494GeneProduct1278378 (Entrez Gene)
AgaP_AGAP011637GeneProduct1280995 (Entrez Gene)
AgaP_AGAP011907GeneProduct1280756 (Entrez Gene)
AgaP_AGAP012054GeneProduct1280613 (Entrez Gene)
AgaP_AGAP012380GeneProduct1280333 (Entrez Gene)
CLK1GeneProductPMID: 15010457: Inhibitor John C. Bell, PhD

Professor, Depts. of Medicine and Biochemistry, Microbiology & Immunology University of Ottawa Career Scientist, Ottawa Regional Cancer Centre Associate Scientist, The Ottawa Hospital Research Institute Telephone: 613-737-7700 ext 6893 Fax: 613-247-3524 Email: John.Bell@orcc.on.ca The control of pre-mRNA splicing by the Clk kinase family

We are studying a family of kinases which we believe provide an interface between intracellular signaling networks and the post-transcriptional mechanism of mRNA splicing. We are performing a structure:function analysis of the three Clk family members to identify domains in the three proteins which are involved in regulating splicing. Using homologous recombination we are generating null strains of mice which are lacking one, two or all three Clk genes. The Clk kinases all possess dual specificity kinase activity and yeast expression systems are being used to produce large amounts of the kinase to perform a detailed analysis of the sites of serine, threonine and tyrosine autophosphorylation within the kinase.
CLK2GeneProduct
CLK4GeneProduct
CPSF4GeneProduct
CUGBP1GeneProduct
DDX20GeneProduct
DICER1GeneProduct
FUSIP1GeneProductFUSI MOUSE PMID: 11684676
GMPMetaboliteHMDB01397 (HMDB)
HNRPA2B1GeneProduct
HNRPA3P1GeneProduct
HNRPCGeneProduct
HNRPH1GeneProduct
HNRPH2GeneProduct
HNRPKGeneProduct
HNRPUGeneProduct
HRMT1L1GeneProduct
NCBP2GeneProduct
NONOGeneProduct
PAPOLAGeneProduct
PCBP2GeneProduct
PRPF4BGeneProduct
PSKH1GeneProductPMID: 12466556
PTBP2GeneProduct
RBMXGeneProduct
RNU2GeneProductGenBank sequence: K00027
SF3A3GeneProduct
SF4GeneProductinferred from: SURP 2, G patch protein, UniProt:O60378
SFRS10GeneProduct
SFRS14GeneProduct
SFRS2GeneProduct
SFRS4GeneProduct
SFRS5GeneProduct
SFRS9GeneProductPMID: 12738786, PMID: 12024014 (associated with hnRNP A1 splicing)
SNRPB2GeneProduct
SNRPEGeneProduct
SNRPNGeneProduct
SRPK2GeneProductPMID: 12773558
SSFA1GeneProduct
U1 snRNARnaGenBank sequence: J00645
U4 snRNAGeneProductGenBank sequence: M10328, M18004
U5 snRNAGeneProductGenBank sequence: AB021173
U5-116KDGeneProduct

Annotated Interactions

No annotated interactions
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