Arginine Biosynthesis (Saccharomyces cerevisiae)

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1, 2L-arginino-succinate2-oxoglutaratefumarateN-acetylglutamyl-phosphateH+N-acetylglutamate semialdehydeN-acetyl-L-glutamateN-acetyl-L-glutamateL-aspartatecarbamoyl-phosphateN-alpha-acetylornithineAMPNADPL-glutamateARG2L-glutamateARG4L-ornithineL-glutamatepyrophosphateCoenzyme AATPphosphateARG5,6NADPHARG3ADPacetyl-CoAcitrullineATPECM40ARG5,6ARG8phosphateARG1L-arginine


Description

The first five steps of arginine biosynthesis in S. cerevisiae take place in the mitochondrion (CITS: [11553611])(CITS: [Hinnebusch]). This part of the pathway is known as the acetylated derivatives cycle because the acetyl group that is added to L-glutamate in the first step of the pathway is recycled via N-acetylglutamate generated in the fifth step. The enzymes that catalyze the second and third steps are encoded by a single gene (ARG5,6) that is translated into a pre-protein which is imported into mitochondria and cleaved there to yield two enzymes, N-acetylglutamate kinase and N-acetylglutamyl-phosphate reductase (CITS: [1649049]). These enzymes form a complex with each other and with N-acetylglutamate synthase, the first enzyme in the pathway, which may have implications for regulation of their activity (CITS: [11553611]). The mitochondrial steps of the arginine biosynthesis pathway result in the formation of ornithine, which is exported to the cytoplasm by the transporter Ort1p (CITS: [8798783]). In the cytoplasm, L-ornithine is converted to L-arginine in three reactions mediated by ornithine carbamoyltransferase, arginosuccinate synthase, and argininosuccinate lyase. Transcription of genes of the arginine biosynthetic pathway, as well as of other amino acid biosynthetic pathways, is activated by the transcription factor Gcn4p under conditions of amino acid starvation (CITS: [11390663])(CITS: [Hinnebusch]). Transcription of ARG1, ARG3, ARG5,6, and ARG8 is also repressed in the presence of arginine by the ArgR/Mcm1p complex, which consists of Arg80p, Arg81p, Arg82p, and Mcm1p (CITS: [14563547]). The transcriptional activator Gcn4p interacts with subunits of the ArgR/Mcm1p repressor, allowing for fine-tuning of transcriptional control in response to arginine availability (CITS: [15289616]). SOURCE: SGD pathways, http://pathway.yeastgenome.org/server.html

Comments

GenMAPP remarks 
Based on http://pathway.yeastgenome.org/biocyc/

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Ontology Terms

 

Bibliography

  1. Pauwels K, Abadjieva A, Hilven P, Stankiewicz A, Crabeel M; ''The N-acetylglutamate synthase/N-acetylglutamate kinase metabolon of Saccharomyces cerevisiae allows co-ordinated feedback regulation of the first two steps in arginine biosynthesis.''; Eur J Biochem, 2003 PubMed Europe PMC
  2. Abadjieva A, Pauwels K, Hilven P, Crabeel M; ''A new yeast metabolon involving at least the two first enzymes of arginine biosynthesis: acetylglutamate synthase activity requires complex formation with acetylglutamate kinase.''; J Biol Chem, 2001 PubMed Europe PMC

History

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CompareRevisionActionTimeUserComment
89705view06:39, 27 September 2016EgonwReplace the CAS number of the salt with that of just the metabolite.
69844view15:49, 11 July 2013EgonwMarked a few DataNodes with CAS registry numbers as metabolites.
69609view20:33, 8 July 2013MaintBotUpdated to 2013 gpml schema
67059view10:00, 26 June 2013Christine ChichesterOntology Term : 'arginine biosynthetic pathway' added !
45186view09:32, 7 October 2011Mkutmonconnect lines
41841view04:50, 2 March 2011MaintBotRemoved redundant pathway information and comments
36640view22:36, 9 April 2010MaintBotDescription and bibliography added from SGD
21257view11:31, 14 November 2008MaintBot[[Pathway:Saccharomyces cerevisiae:Arginine Biosynthesis]] moved to [[Pathway:WP275]]: Moved to stable identifier
12656view07:56, 17 May 2008MaintBotautomated metabolite conversion
8645view14:05, 7 January 2008MaintBotAdded to category $category
8643view14:05, 7 January 2008M.BraymerUploaded new pathway

External references

DataNodes

View all...
NameTypeDatabase referenceComment
ADPMetabolite58-64-0 (CAS)
ARG1GeneProductS000005419 (SGD)
ARG2GeneProductS000003607 (SGD)
ARG3GeneProductS000003624 (SGD)
ARG4GeneProductS000001060 (SGD)
ARG5,6GeneProductS000000871 (SGD)
ARG8GeneProductS000005500 (SGD)
ATPMetabolite1927-31-7 (CAS)
Coenzyme AMetabolite64885-97-8 (CAS)
ECM40GeneProductS000004666 (SGD)
L-arginineMetabolite74-79-3 (CAS)
L-glutamateMetabolite56-86-0 (CAS)
L-ornithineMetabolite70-26-8 (CAS)
NADPHMetabolite53-57-6 (CAS)
acetyl-CoAMetabolite72-89-9 (CAS)
citrullineMetabolite372-75-8 (CAS)
phosphateMetabolite14265-44-2 (CAS)
pyrophosphateMetabolite2466-09-3 (CAS)

Annotated Interactions

No annotated interactions
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