Signaling by NOTCH3 (Homo sapiens)

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1, 4, 5, 9, 11...22716332, 7, 221522272088332610262227106, 192930301529Signal receiving cellcytosolSignal sending cellnucleoplasmcytosolPSEN1(1-298) UBC(609-684) KAT2A MAML2 WWP2RBPJ MAML2 UBC(381-456) HES5IK1 MAML2 NICD3 MAML2 NOTCH1,NOTCH3coactivatorcomplexesNICD3 DLL1 IK1:PTCRA geneNICD3Ub-NICD3 PLXND1UBB(1-76) PLXND1 gene HEY1 gene MAML2 RBPJ FABP7 geneADAM10:Zn2+DLL/JAG:NOTCH3UbNOTCH3 coactivatorcomplex:HES1 genep-Y701-STAT1 DLL4 NOTCH3(1572-2321) HES1 gene NICD3 NEURL PSENEN SNW1 MAML1 NICD3 MAMLD1 NICD1 MAML3 UBB(77-152) HEYL Gene DLGAP5 gene MAMLD1 NOTCH3coactivatorcomplex:p-Y701-STAT1 dimer:HES1 geneMAMLD1 Ub-DLL1 p-Y701-STAT1 KAT2B UBA52(1-76) UBB(1-76) UBA52(1-76) NICD3EP300 RBPJ UBC(305-380) NOTCH3(1572-2321) JAG2 EP300 MAML3 UBC(533-608) HES5 geneCREBBP HEYL GeneKAT2B NEXT3 MAML3 DLGAP5 geneUb-DLL/JAG:NOTCH3(40-1628)HEY2 geneAPH1B MAML1 MAML1 RPS27A(1-76) UBC(77-152) ADPUBB(153-228) UBC(229-304) UBC(457-532) JAG1 MAMLD1 NICD3 HES1 gene NOTCH3 coactivatorcomplex:PBX1 genePLXND1 geneRBPJ NEXT3p-Y701-STAT1 UBB(153-228) PBX1 gene HEY114xGlcS-10xFucT-4xFucS-NOTCH3(40-1571) NEXT3 APH1A DLL4 PBX1 genePSEN1(299-467) p-Y701-STAT1dimer:HEY1 geneNOTCH3 coactivatorcomplex:DLGAP5 geneNICD3 NICD1 Ub-NEXT3,Ub-NICD3:WWP2NICD3 SNW1 JAG2:NOTCH3HEY1 geneYBX1 MAML3 JAG2 MAMLD1 DLL1PTCRAHEY2EGF:p-6Y-EGFR dimerUb-DLL4 MAML2 MIB2 p-6Y-EGFR Zn2+ UBC(153-228) IK1Ub-JAG1 PBX1NCSTN HEYLNICD3 JAG1 Ub-JAG1 Ub-DLL4 PTCRA gene KAT2A UbMAMLNICD3 UBC(77-152) YBX1PSEN2(1-297) NOTCH3 coactivatorcomplex:WWC1 geneWWP2 EGF NICD3:TACC3UBC(153-228) TACC3Ub-DLL1 NOTCH3(1572-2321) MAML3 MAML1 14xGlcS-10xFucT-4xFucS-NOTCH3(40-1571) MAML1 UBC(381-456) DLGAP5MAML2 gamma-secretasecomplexYBX1:NOTCH3UBC(533-608) UBC(229-304) RPS27A(1-76) MAMLD1 Ub-JAG2 MAML3 NEXT3,NICD3:WWP2RBPJ RBPJ MIB1 WWP2 FABP7p-Y-NICD3NEXT3,NICD3MAML1 JAG1MAML1 p-Y701-STAT1 dimerNICD3 MAML3 PTCRA geneNOTCH1,NOTCH3coactivatorcomplex:PLXND1 geneUb-NEXT3 UBC(305-380) MAMLD1 ATPNOTCH3 coactivatorcomplex:PTCRA geneRBPJDLL1 NOTCH3(1572-2321) NOTCH3(40-1628) NICD3 NOTCH3 coactivatorcomplexNICD3 MAML2 NOTCH3EGF:p-6Y-EGFRdimer:NICD3PSEN2(298-448) HES1 geneMAML1 HES1NICD3 UBC(1-76) UBC(1-76) NEURL1B 14xGlcS-10xFucT-4xFucS-NOTCH3(40-1571) 14xGlcS-10xFucT-4xFucS-NOTCH3(40-1571) Ub-JAG2 CREBBP MAML3 14xGlcS-10xFucT-4xFucS-NOTCH3(40-1571) UBB(77-152) TACC3 JAG2MAML3 NOTCH3(1629-1661)WWC1 geneMAML2 NOTCH3 coactivatorcomplex:HEYL genep-6Y-EGFR MAMLD1 MIB/NEURLNOTCH3(1572-2321) NOTCH3(1572-2321) 14xGlcS-10xFucT-4xFucS-NOTCH3(40-1571) MAML3 MAML2 MAML1 WWC1 gene UBC(457-532) EGF DLL4:NOTCH3MAML3 MAMLD1 PTCRA gene MAML2 MAMLD1 DLL1:NOTCH3UBC(609-684) JAG1:NOTCH3NOTCH3(1572-2321) ADAM10 Ub-DLL/JAG:NOTCH3RBPJ NOTCH3(1572-2321) DLL4MAMLD1 14xGlcS-10xFucT-4xFucS-NOTCH3(40-1571) 14xGlcS-10xFucT-4xFucS-NOTCH3(40-1571) WWC1RBPJ MAML1 RBPJ RBPJ MAML1 NICD3 3222782933


Description

Similar to NOTCH1, NOTCH3 is activated by delta-like and jagged ligands (DLL/JAG) expressed in trans on a neighboring cell. The activation triggers cleavage of NOTCH3, first by ADAM10 at the S2 cleavage site, then by gamma-secretase at the S3 cleavage site, resulting in the release of the intracellular domain of NOTCH3, NICD3, into the cytosol. NICD3 subsequently traffics to the nucleus where it acts as a transcriptional regulator. NOTCH3 expression pattern is more restricted than the expression patterns of NOTCH1 and NOTCH2, with predominant expression of NOTCH3 in vascular smooth muscle cells, lymphocytes and the nervous system (reviewed by Bellavia et al. 2008). Based on the study of Notch3 knockout mice, Notch3 is not essential for embryonic development or fertility (Krebs et al. 2003).

<p>Germline gain-of-function NOTCH3 mutations are an underlying cause of the CADASIL syndrome - cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy. CADASIL is characterized by degeneration and loss of vascular smooth muscle cells from the arterial wall, predisposing affected individuals to an early onset stroke (Storkebaum et al. 2011). NOTCH3 promotes survival of vascular smooth muscle cells at least in part by induction of CFLAR (c FLIP), an inhibitor of FASLG activated death receptor signaling. The mechanism of NOTCH3 mediated upregulation of CFLAR is unknown; it is independent of the NOTCH3 coactivator complex and involves an unelucidated crosstalk with the RAS/RAF/MAPK pathway (Wang et al. 2002).<p><p>In rat brain, NOTCH3 and NOTCH1 are expressed at sites of adult neurogenesis, such as the dentate gyrus (Irvin et al. 2001). NOTCH3, similar to NOTCH1, promotes differentiation of the rat adult hippocampus derived multipotent neuronal progenitors into astroglia (Tanigaki et al. 2001). NOTCH1, NOTCH2, NOTCH3, and their ligand DLL1 are expressed in neuroepithelial precursor cells in the neural tube of mouse embryos. Together, they signal to inhibit neuronal differentiation of neuroepithelial precursors. Expression of NOTCH3 in mouse neuroepithelial precursors is stimulated by growth factors BMP2, FGF2, Xenopus TGF beta5 - homologous to TGFB1, LIF, and NTF3 (Faux et al. 2001).<p>In mouse telencephalon, NOTCH3, similar to NOTCH1, promotes radial glia and neuronal progenitor phenotype. This can, at least in part be attributed to NOTCH mediated activation of RBPJ-dependent and HES5-dependent transcription (Dang et al. 2006).<p>In mouse spinal cord, Notch3 is involved in neuronal differentiation and maturation. Notch3 knockout mice have a decreased number of mature inhibitory interneurons in the spinal cord, which may be involved in chronic pain conditions (Rusanescu and Mao 2014).<p><p>NOTCH3 amplification was reported in breast cancer, where NOTCH3 promotes proliferation and survival of ERBB2 negative breast cancer cells (Yamaguchi et al. 2008), and it has also been reported in ovarian cancer (Park et al. 2006). NOTCH3 signaling is involved in TGF beta (TGFB1) signaling-induced eptihelial to mesenchimal transition (EMT) (Ohashi et al. 2011, Liu et al. 2014)<p><p>NOTCH3 indirectly promotes development of regulatory T cells (Tregs). NOTCH3 signaling activates pre-TCR-dependent and PKC-theta (PRKCQ)-dependent NF-kappaB (NFKB) activation, resulting in induction of FOXP3 expression (Barbarulo et al. 2011). Deregulated NOTCH3 and pre-TCR signaling contributes to development of leukemia and lymphoma (Bellavia et al. 2000, Bellavia et al. 2002). View original pathway at Reactome.</div>

Comments

Reactome-Converter 
Pathway is converted from Reactome ID: 9012852
Reactome-version 
Reactome version: 75
Reactome Author 
Reactome Author: Jassal, Bijay

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Ontology Terms

 

Bibliography

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History

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CompareRevisionActionTimeUserComment
114804view16:30, 25 January 2021ReactomeTeamReactome version 75
113248view11:31, 2 November 2020ReactomeTeamReactome version 74
112466view15:41, 9 October 2020ReactomeTeamReactome version 73
101375view11:26, 1 November 2018ReactomeTeamreactome version 66
100913view21:01, 31 October 2018ReactomeTeamreactome version 65
100454view19:36, 31 October 2018ReactomeTeamreactome version 64
100001view16:19, 31 October 2018ReactomeTeamreactome version 63
99001view15:17, 22 October 2018DeSlchanged reactome ID number, since old number no longer exists.
93913view13:44, 16 August 2017ReactomeTeamreactome version 61
93489view11:25, 9 August 2017ReactomeTeamreactome version 61
87182view19:57, 18 July 2016EgonwOntology Term : 'signaling pathway' added !
86585view09:21, 11 July 2016ReactomeTeamreactome version 56
83126view10:02, 18 November 2015ReactomeTeamVersion54
81466view13:00, 21 August 2015ReactomeTeamVersion53
76939view08:21, 17 July 2014ReactomeTeamFixed remaining interactions
76644view12:01, 16 July 2014ReactomeTeamFixed remaining interactions
75974view10:03, 11 June 2014ReactomeTeamRe-fixing comment source
75677view11:00, 10 June 2014ReactomeTeamReactome 48 Update
75032view13:54, 8 May 2014AnweshaFixing comment source for displaying WikiPathways description
74676view08:44, 30 April 2014ReactomeTeamNew pathway

External references

DataNodes

View all...
NameTypeDatabase referenceComment
14xGlcS-10xFucT-4xFucS-NOTCH3(40-1571) ProteinQ9UM47 (Uniprot-TrEMBL)
ADAM10 ProteinO14672 (Uniprot-TrEMBL)
ADAM10:Zn2+ComplexR-HSA-157186 (Reactome)
ADPMetaboliteCHEBI:456216 (ChEBI)
APH1A ProteinQ96BI3 (Uniprot-TrEMBL)
APH1B ProteinQ8WW43 (Uniprot-TrEMBL)
ATPMetaboliteCHEBI:30616 (ChEBI)
CREBBP ProteinQ92793 (Uniprot-TrEMBL)
DLGAP5 gene ProteinENSG00000126787 (Ensembl)
DLGAP5 geneGeneProductENSG00000126787 (Ensembl)
DLGAP5ProteinQ15398 (Uniprot-TrEMBL)
DLL/JAG:NOTCH3ComplexR-HSA-9013071 (Reactome)
DLL1 ProteinO00548 (Uniprot-TrEMBL)
DLL1:NOTCH3ComplexR-HSA-157136 (Reactome)
DLL1ProteinO00548 (Uniprot-TrEMBL)
DLL4 ProteinQ9NR61 (Uniprot-TrEMBL)
DLL4:NOTCH3ComplexR-HSA-2168132 (Reactome)
DLL4ProteinQ9NR61 (Uniprot-TrEMBL)
EGF ProteinP01133 (Uniprot-TrEMBL)
EGF:p-6Y-EGFR dimer:NICD3ComplexR-HSA-9018569 (Reactome)
EGF:p-6Y-EGFR dimerComplexR-HSA-179882 (Reactome)
EP300 ProteinQ09472 (Uniprot-TrEMBL)
FABP7 geneGeneProductENSG00000164434 (Ensembl)
FABP7ProteinO15540 (Uniprot-TrEMBL)
HES1 gene ProteinENSG00000114315 (Ensembl)
HES1 geneGeneProductENSG00000114315 (Ensembl)
HES1ProteinQ14469 (Uniprot-TrEMBL)
HES5 geneGeneProductENSG00000197921 (Ensembl)
HES5ProteinQ5TA89 (Uniprot-TrEMBL)
HEY1 gene ProteinENSG00000164683 (Ensembl)
HEY1 geneGeneProductENSG00000164683 (Ensembl)
HEY1ProteinQ9Y5J3 (Uniprot-TrEMBL)
HEY2 geneGeneProductENSG00000135547 (Ensembl)
HEY2ProteinQ9UBP5 (Uniprot-TrEMBL)
HEYL Gene ProteinENSG00000163909 (Ensembl)
HEYL GeneGeneProductENSG00000163909 (Ensembl)
HEYLProteinQ9NQ87 (Uniprot-TrEMBL)
IK1 ProteinQ13422-1 (Uniprot-TrEMBL)
IK1:PTCRA geneComplexR-HSA-9017116 (Reactome)
IK1ProteinQ13422-1 (Uniprot-TrEMBL)
JAG1 ProteinP78504 (Uniprot-TrEMBL)
JAG1:NOTCH3ComplexR-HSA-157132 (Reactome)
JAG1ProteinP78504 (Uniprot-TrEMBL)
JAG2 ProteinQ9Y219 (Uniprot-TrEMBL)
JAG2:NOTCH3ComplexR-HSA-157066 (Reactome)
JAG2ProteinQ9Y219 (Uniprot-TrEMBL)
KAT2A ProteinQ92830 (Uniprot-TrEMBL)
KAT2B ProteinQ92831 (Uniprot-TrEMBL)
MAML1 ProteinQ92585 (Uniprot-TrEMBL)
MAML2 ProteinQ8IZL2 (Uniprot-TrEMBL)
MAML3 ProteinQ96JK9 (Uniprot-TrEMBL)
MAMLD1 ProteinQ13495 (Uniprot-TrEMBL)
MAMLComplexR-HSA-212357 (Reactome)
MIB/NEURLComplexR-HSA-1911464 (Reactome)
MIB1 ProteinQ86YT6 (Uniprot-TrEMBL)
MIB2 ProteinQ96AX9 (Uniprot-TrEMBL)
NCSTN ProteinQ92542 (Uniprot-TrEMBL)
NEURL ProteinO76050 (Uniprot-TrEMBL)
NEURL1B ProteinA8MQ27 (Uniprot-TrEMBL)
NEXT3 ProteinQ9UM47 (Uniprot-TrEMBL)
NEXT3,NICD3:WWP2ComplexR-HSA-9021521 (Reactome)
NEXT3,NICD3ComplexR-HSA-9021530 (Reactome)
NEXT3ProteinQ9UM47 (Uniprot-TrEMBL)
NICD1 ProteinP46531 (Uniprot-TrEMBL)
NICD3 ProteinQ9UM47 (Uniprot-TrEMBL)
NICD3:TACC3ComplexR-HSA-9017852 (Reactome)
NICD3ProteinQ9UM47 (Uniprot-TrEMBL)
NOTCH1,NOTCH3

coactivator

complex:PLXND1 gene
ComplexR-HSA-9021452 (Reactome)
NOTCH1,NOTCH3

coactivator

complexes
ComplexR-HSA-9016789 (Reactome)
NOTCH3

coactivator

complex:p-Y701-STAT1 dimer:HES1 gene
ComplexR-HSA-9021313 (Reactome)
NOTCH3 coactivator complex:DLGAP5 geneComplexR-HSA-9018547 (Reactome)
NOTCH3 coactivator complex:HES1 geneComplexR-HSA-9013653 (Reactome)
NOTCH3 coactivator complex:HEYL geneComplexR-HSA-9017405 (Reactome)
NOTCH3 coactivator complex:PBX1 geneComplexR-HSA-9021411 (Reactome)
NOTCH3 coactivator complex:PTCRA geneComplexR-HSA-9016914 (Reactome)
NOTCH3 coactivator complex:WWC1 geneComplexR-HSA-9021490 (Reactome)
NOTCH3 coactivator complexComplexR-HSA-2248837 (Reactome)
NOTCH3(1572-2321) ProteinQ9UM47 (Uniprot-TrEMBL)
NOTCH3(1629-1661)ProteinQ9UM47 (Uniprot-TrEMBL)
NOTCH3(40-1628) ProteinQ9UM47 (Uniprot-TrEMBL)
NOTCH3ComplexR-HSA-157048 (Reactome)
PBX1 gene ProteinENSG00000185630 (Ensembl)
PBX1 geneGeneProductENSG00000185630 (Ensembl)
PBX1ProteinP40424 (Uniprot-TrEMBL)
PLXND1 gene ProteinENSG00000004399 (Ensembl)
PLXND1 geneGeneProductENSG00000004399 (Ensembl)
PLXND1ProteinQ9Y4D7 (Uniprot-TrEMBL)
PSEN1(1-298) ProteinP49768 (Uniprot-TrEMBL)
PSEN1(299-467) ProteinP49768 (Uniprot-TrEMBL)
PSEN2(1-297) ProteinP49810 (Uniprot-TrEMBL)
PSEN2(298-448) ProteinP49810 (Uniprot-TrEMBL)
PSENEN ProteinQ9NZ42 (Uniprot-TrEMBL)
PTCRA gene ProteinENSG00000171611 (Ensembl)
PTCRA geneGeneProductENSG00000171611 (Ensembl)
PTCRAProteinQ6ISU1 (Uniprot-TrEMBL)
RBPJ ProteinQ06330 (Uniprot-TrEMBL)
RBPJProteinQ06330 (Uniprot-TrEMBL)
RPS27A(1-76) ProteinP62979 (Uniprot-TrEMBL)
SNW1 ProteinQ13573 (Uniprot-TrEMBL)
TACC3 ProteinQ9Y6A5 (Uniprot-TrEMBL)
TACC3ProteinQ9Y6A5 (Uniprot-TrEMBL)
UBA52(1-76) ProteinP62987 (Uniprot-TrEMBL)
UBB(1-76) ProteinP0CG47 (Uniprot-TrEMBL)
UBB(153-228) ProteinP0CG47 (Uniprot-TrEMBL)
UBB(77-152) ProteinP0CG47 (Uniprot-TrEMBL)
UBC(1-76) ProteinP0CG48 (Uniprot-TrEMBL)
UBC(153-228) ProteinP0CG48 (Uniprot-TrEMBL)
UBC(229-304) ProteinP0CG48 (Uniprot-TrEMBL)
UBC(305-380) ProteinP0CG48 (Uniprot-TrEMBL)
UBC(381-456) ProteinP0CG48 (Uniprot-TrEMBL)
UBC(457-532) ProteinP0CG48 (Uniprot-TrEMBL)
UBC(533-608) ProteinP0CG48 (Uniprot-TrEMBL)
UBC(609-684) ProteinP0CG48 (Uniprot-TrEMBL)
UBC(77-152) ProteinP0CG48 (Uniprot-TrEMBL)
Ub-DLL/JAG:NOTCH3(40-1628)ComplexR-HSA-9013287 (Reactome)
Ub-DLL/JAG:NOTCH3ComplexR-HSA-9013089 (Reactome)
Ub-DLL1 ProteinO00548 (Uniprot-TrEMBL)
Ub-DLL4 ProteinQ9NR61 (Uniprot-TrEMBL)
Ub-JAG1 ProteinP78504 (Uniprot-TrEMBL)
Ub-JAG2 ProteinQ9Y219 (Uniprot-TrEMBL)
Ub-NEXT3 ProteinQ9UM47 (Uniprot-TrEMBL)
Ub-NEXT3,Ub-NICD3:WWP2ComplexR-HSA-9021524 (Reactome)
Ub-NICD3 ProteinQ9UM47 (Uniprot-TrEMBL)
UbComplexR-HSA-113595 (Reactome)
WWC1 gene ProteinENSG00000113645 (Ensembl)
WWC1 geneGeneProductENSG00000113645 (Ensembl)
WWC1ProteinQ8IX03 (Uniprot-TrEMBL)
WWP2 ProteinO00308 (Uniprot-TrEMBL)
WWP2ProteinO00308 (Uniprot-TrEMBL)
YBX1 ProteinP67809 (Uniprot-TrEMBL)
YBX1:NOTCH3ComplexR-HSA-9017807 (Reactome)
YBX1ProteinP67809 (Uniprot-TrEMBL)
Zn2+ MetaboliteCHEBI:29105 (ChEBI)
gamma-secretase complexComplexR-HSA-157343 (Reactome)
p-6Y-EGFR ProteinP00533 (Uniprot-TrEMBL)
p-Y-NICD3ProteinQ9UM47 (Uniprot-TrEMBL)
p-Y701-STAT1 dimer:HEY1 geneComplexR-HSA-9021333 (Reactome)
p-Y701-STAT1 ProteinP42224 (Uniprot-TrEMBL)
p-Y701-STAT1 dimerComplexR-HSA-6788626 (Reactome)

Annotated Interactions

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SourceTargetTypeDatabase referenceComment
ADAM10:Zn2+mim-catalysisR-HSA-9013284 (Reactome)
ADPArrowR-HSA-9018572 (Reactome)
ATPR-HSA-9018572 (Reactome)
DLGAP5 geneR-HSA-9018538 (Reactome)
DLGAP5 geneR-HSA-9018542 (Reactome)
DLGAP5ArrowR-HSA-9018538 (Reactome)
DLL/JAG:NOTCH3R-HSA-9013069 (Reactome)
DLL1:NOTCH3ArrowR-HSA-157145 (Reactome)
DLL1R-HSA-157145 (Reactome)
DLL4:NOTCH3ArrowR-HSA-2168136 (Reactome)
DLL4R-HSA-2168136 (Reactome)
EGF:p-6Y-EGFR dimer:NICD3ArrowR-HSA-9018573 (Reactome)
EGF:p-6Y-EGFR dimer:NICD3R-HSA-9018572 (Reactome)
EGF:p-6Y-EGFR dimer:NICD3mim-catalysisR-HSA-9018572 (Reactome)
EGF:p-6Y-EGFR dimerArrowR-HSA-9018572 (Reactome)
EGF:p-6Y-EGFR dimerR-HSA-9018573 (Reactome)
FABP7 geneR-HSA-9016751 (Reactome)
FABP7ArrowR-HSA-9016751 (Reactome)
HES1 geneR-HSA-9013567 (Reactome)
HES1 geneR-HSA-9013652 (Reactome)
HES1 geneR-HSA-9021325 (Reactome)
HES1ArrowR-HSA-9013567 (Reactome)
HES5 geneR-HSA-9013568 (Reactome)
HES5ArrowR-HSA-9013568 (Reactome)
HEY1 geneR-HSA-9014129 (Reactome)
HEY1 geneR-HSA-9021334 (Reactome)
HEY1ArrowR-HSA-9014129 (Reactome)
HEY2 geneR-HSA-9014141 (Reactome)
HEY2ArrowR-HSA-9014141 (Reactome)
HEYL GeneR-HSA-9013488 (Reactome)
HEYL GeneR-HSA-9017398 (Reactome)
HEYLArrowR-HSA-9013488 (Reactome)
IK1:PTCRA geneArrowR-HSA-9017120 (Reactome)
IK1:PTCRA geneTBarR-HSA-9016861 (Reactome)
IK1R-HSA-9017120 (Reactome)
JAG1:NOTCH3ArrowR-HSA-157100 (Reactome)
JAG1R-HSA-157100 (Reactome)
JAG2:NOTCH3ArrowR-HSA-157124 (Reactome)
JAG2R-HSA-157124 (Reactome)
MAMLR-HSA-9013631 (Reactome)
MIB/NEURLmim-catalysisR-HSA-9013069 (Reactome)
NEXT3,NICD3:WWP2ArrowR-HSA-9021520 (Reactome)
NEXT3,NICD3:WWP2R-HSA-9021523 (Reactome)
NEXT3,NICD3:WWP2mim-catalysisR-HSA-9021523 (Reactome)
NEXT3,NICD3R-HSA-9021520 (Reactome)
NEXT3ArrowR-HSA-9013284 (Reactome)
NEXT3R-HSA-9013361 (Reactome)
NICD3:TACC3ArrowR-HSA-9017855 (Reactome)
NICD3ArrowR-HSA-157937 (Reactome)
NICD3ArrowR-HSA-9013361 (Reactome)
NICD3ArrowR-HSA-9017817 (Reactome)
NICD3R-HSA-157937 (Reactome)
NICD3R-HSA-9013631 (Reactome)
NICD3R-HSA-9017855 (Reactome)
NICD3R-HSA-9018573 (Reactome)
NOTCH1,NOTCH3

coactivator

complex:PLXND1 gene
ArrowR-HSA-9021450 (Reactome)
NOTCH1,NOTCH3

coactivator

complex:PLXND1 gene
ArrowR-HSA-9021451 (Reactome)
NOTCH1,NOTCH3

coactivator

complexes
ArrowR-HSA-9016751 (Reactome)
NOTCH1,NOTCH3

coactivator

complexes
R-HSA-9021451 (Reactome)
NOTCH3

coactivator

complex:p-Y701-STAT1 dimer:HES1 gene
ArrowR-HSA-9013567 (Reactome)
NOTCH3

coactivator

complex:p-Y701-STAT1 dimer:HES1 gene
ArrowR-HSA-9021325 (Reactome)
NOTCH3 coactivator complex:DLGAP5 geneArrowR-HSA-9018538 (Reactome)
NOTCH3 coactivator complex:DLGAP5 geneArrowR-HSA-9018542 (Reactome)
NOTCH3 coactivator complex:HES1 geneArrowR-HSA-9013567 (Reactome)
NOTCH3 coactivator complex:HES1 geneArrowR-HSA-9013652 (Reactome)
NOTCH3 coactivator complex:HEYL geneArrowR-HSA-9013488 (Reactome)
NOTCH3 coactivator complex:HEYL geneArrowR-HSA-9017398 (Reactome)
NOTCH3 coactivator complex:PBX1 geneArrowR-HSA-9021406 (Reactome)
NOTCH3 coactivator complex:PBX1 geneArrowR-HSA-9021412 (Reactome)
NOTCH3 coactivator complex:PTCRA geneArrowR-HSA-9016861 (Reactome)
NOTCH3 coactivator complex:PTCRA geneArrowR-HSA-9016916 (Reactome)
NOTCH3 coactivator complex:WWC1 geneArrowR-HSA-9021478 (Reactome)
NOTCH3 coactivator complex:WWC1 geneArrowR-HSA-9021480 (Reactome)
NOTCH3 coactivator complexArrowR-HSA-9013568 (Reactome)
NOTCH3 coactivator complexArrowR-HSA-9013631 (Reactome)
NOTCH3 coactivator complexArrowR-HSA-9014129 (Reactome)
NOTCH3 coactivator complexArrowR-HSA-9014141 (Reactome)
NOTCH3 coactivator complexR-HSA-9013652 (Reactome)
NOTCH3 coactivator complexR-HSA-9016916 (Reactome)
NOTCH3 coactivator complexR-HSA-9017398 (Reactome)
NOTCH3 coactivator complexR-HSA-9018542 (Reactome)
NOTCH3 coactivator complexR-HSA-9021325 (Reactome)
NOTCH3 coactivator complexR-HSA-9021406 (Reactome)
NOTCH3 coactivator complexR-HSA-9021480 (Reactome)
NOTCH3(1629-1661)ArrowR-HSA-9013361 (Reactome)
NOTCH3R-HSA-157100 (Reactome)
NOTCH3R-HSA-157124 (Reactome)
NOTCH3R-HSA-157145 (Reactome)
NOTCH3R-HSA-2168136 (Reactome)
NOTCH3R-HSA-9017797 (Reactome)
PBX1 geneR-HSA-9021406 (Reactome)
PBX1 geneR-HSA-9021412 (Reactome)
PBX1ArrowR-HSA-9021412 (Reactome)
PLXND1 geneR-HSA-9021450 (Reactome)
PLXND1 geneR-HSA-9021451 (Reactome)
PLXND1ArrowR-HSA-9021450 (Reactome)
PTCRA geneR-HSA-9016861 (Reactome)
PTCRA geneR-HSA-9016916 (Reactome)
PTCRA geneR-HSA-9017120 (Reactome)
PTCRAArrowR-HSA-9016861 (Reactome)
R-HSA-157100 (Reactome) Based on a study with mouse proteins, NOTCH3 receptor binds to JAG1 (Jagged1) ligand (Shimizu et al. 1999).
R-HSA-157124 (Reactome) Based on a study with mouse NOTCH3 and presumably mouse JAG2 (Jagged2), JAG2 ligand binds to NOTCH3 receptor (Shimizu et al. 2000).
R-HSA-157145 (Reactome) Based on a study with mouse proteins, NOTCH3 receptor binds to DLL1 ligand (Shimizu et al. 2000). The interaction of DLL1 and NOTCH3 is implicated in functional differentiation of activated CD4+ T lymphocytes into type 1 helper T cells (Th1) (Maekawa et al. 2003). The effect of Fringe-mediated modification of NOTCH3 on its interaction with DLL1 is poorly studied (Hou et al. 2012).
R-HSA-157937 (Reactome) Based on an analogy with Drosophila Notch and its intracellular cleavage product NICD, it is believed that the cytosolic NICD3 translocates to the nucleus (Lecourtois and Schweisguth 1998, Struhl and Adachi 1998).
R-HSA-2168136 (Reactome) Binding of NOTCH3 receptor to DLL4 ligand has not been directly demonstrated. DLL4 and NOTCH3 are expressed on neighboring cells in retina (Claxton and Fruttiger 2004) and in endothelium/blood (Indraccolo et al. 2009), and DLL4 significantly and specifically increases NOTCH3 signaling (Indraccolo et al. 2009).
R-HSA-9013069 (Reactome) Based on an analogy with NOTCH1 and NOTCH2, and on studies of Drosophila Notch, NOTCH ligands DLL1, DLL4, JAG1 and JAG2 are assumed to undergo ubiquitination and endocytosis after binding to NOTCH3 in trans. Ubiquitination of DLL/JAG ligands in mammals is performed by orthologues of Drosophila Mindbomb, MIB1, possibly MIB2, and possibly orthologues of Drosophila Neuralized, NEURL and NEURL1B.
R-HSA-9013284 (Reactome) Ligand binding induces a conformational change in NOTCH3, probably through mechanical pulling of NOTCH3 triggered by endocytosis of the receptor-attached ubiquitinated ligand. This conformational change exposes the S2 site in the extracellular region of NOTCH3 and triggers cleavage of NOTCH3 by ADAM10 metalloprotease, generating the membrane-anchored NOTCH3 fragment NEXT3 (Groot et al. 2014). The extracellular NOTCH3 portion remains attached to the ligand presented on the plasma membrane of a neighboring cell.
R-HSA-9013361 (Reactome) NEXT3 fragment of NOTCH3 is further cleaved at the S3 site by the gamma-secretase complex, containing either PSEN1 (presenilin-1) or PSEN2 (presenilin-2) as the catalytic subunit, which releases the intracellular domain NICD3 into the cytosol (Groot et al. 2014).
R-HSA-9013488 (Reactome) HEYL gene expression is stimulated by NOTCH3 (Maier and Gessler 2000). In developing mouse embryos, Notch3 expression overlaps with HeyL expression in vascular smooth muscle cells and in the thymus (Leimeister et al. 2000).
R-HSA-9013567 (Reactome) Based on studies in a mouse system, HES1 gene expression is directly stimulated by the NOTCH3 coactivator complex (Lin et al. 2002, Shimizu et al. 2002). Activated STAT1 directly enhances HES1 transcription induced by NICD3 (Boelens et al. 2014).
R-HSA-9013568 (Reactome) Based on studies in mouse, NOTCH3 intracellular domain (NICD3), likely in complex with RBPJ and MAML (MAML1, MAML2 or MAML3), positively regulates transcription from the HES5 gene promoter. While HES5 promoter contains RBPJ binding elements, direct binding of the NOTCH3 coactivator complex to the HES5 promoter has not been demonstrated (Lin et al. 2002, Shimizu et al. 2002). Studies in rats suggest that NOTCH3-mediated upregulation of HES5 plays a key role in the development of pulmonary arterial hypertension. NOTCH3 and HES5 are both expressed in vascular smooth muscle cells of small pulmonary arteries of human and rat lungs, with NOTCH3 and HES5 levels increased in hypertensive human and rat lung tissues (Li et al. 2009).
R-HSA-9013631 (Reactome) In the nucleus, NICD3 forms a complex with RBPJ (CBF1, CSL) and MAML (mastermind) proteins MAML1, MAML2 or MAML3 (possibly also MAMLD1). NICD3:RBPJ:MAML complex activates transcription from RBPJ-binding promoter elements (Lin et al. 2002).

Besides NICD3, RBPJ and MAML, NOTCH3 coactivator complex likely includes other proteins shown as components of the NOTCH1 coactivator complex. Since disruption of the RBPJ:NCOR corepressor and MAML-mediated recruitment of transcriptional activators has not been studied in the context of NICD3, it is not shown here. More details are available in the pathway Signaling by NOTCH1.

Many NOTCH-regulated genes have paired RBPJ-binding sites in their promoters, in head-to-head arrangement, and require cooperative formation of dimeric NOTCH transcription complexes for transcriptional activation (Nam et al. 2007).
R-HSA-9013652 (Reactome) NOTCH3 coactivator complex, composed of at least NICD3 (NOTCH3 intracellular domain), RBPJ and MAML (MAML1, MAML2 or MAML3; possibly MAMLD1), directly binds to RBPJ elements in the HES1 gene promoter (Lin et al. 2002).
R-HSA-9014129 (Reactome) Based on a study involving mouse and rat proteins and DNA, NOTCH3 coactivator complex positively regulates transcription of HEY1 (HRT1). Direct binding of NOTCH3 to the HEY1 gene promoter has not been demonstrated (Wang et al. 2002). STAT1 directly enhances HEY1 gene transcription induced by NICD3. In breast cancer, stromal cells secrete RNA-containing exosomes. Upon uptake of exosomes by breast cancer cells, STAT1-dependent antiviral signaling is initiated. Stromal cells also express JAG1 ligand on their surface and, in parallel to STAT1 signaling, activate NOTCH3 signaling in NOTCH3-expressing breast cancer cells. Synergistic activation of NOTCH3 and STAT1 increases breast cancer radiation resistance (Boelens et al. 2014).
R-HSA-9014141 (Reactome) Based on a study involving mouse and rat proteins and DNA, NOTCH3 coactivator complex positively regulates transcription of HEY2 (HRT2). Direct binding of NOTCH3 to the HEY2 gene promoter has not been demonstrated (Wang et al. 2002).
R-HSA-9016751 (Reactome) Based on studies in mice, expression of FABP7 (BLBP) in radial glia is positively regulated by NOTCH1 and NOTCH3 during neuronal migration (Anthony et al. 2005, Keilani and Sugaya 2008). The promoter of the mouse Fabp7 gene contains an Rbpj binding site needed for Fabp7 expression (Anthony et al. 2005). Several sites related to the consensus RBPJ binding sequence exist in the human FABP7 gene promoter.
R-HSA-9016861 (Reactome) Based on studies in mice, NOTCH3 intracellular domain NICD3 positively regulates transcription of the PTCRA gene, encoding pre-T-cell receptor alpha (pre-TCR-alpha or pTalpha). NICD3-mediated induction of PTCRA transcription is RBPJ and MAML dependent, and the PTCRA promoter contains several RBPJ-binding sites (Talora et al. 2003, Bellavia et al. 2007). IK1, splicing isoform of the transcription factor Ikaros (IKZF1), competes with RBPJ for binding to the PTCRA promoter and inhibits PTCRA transcription. NOTCH3, through pre-TCR signaling, stimulates expression of the RNA binding protein HuD, which promotes splicing of IKZF1 into dominant negative isoforms. These dominant negative isoforms of IKZF1 heterodimerize with IK1, preventing its binding to target DNA sequences and thus contributing to sustained transcription of PTCRA (Bellavia et al. 2007, reviewed by Bellavia, Mecarrozzi, Campese, Grazioli, Gulino and Screpanti 2007).
R-HSA-9016916 (Reactome) Based on studies in mice, NOTCH3 coactivator complex binds to RBPJ binding sites in the promoter of the PTCRA gene, encoding pre-TCR-alpha (Bellavia et al. 2007).
R-HSA-9017120 (Reactome) IK1, the splicing isoform 1 of the transcription factor Ikaros (IKZF1), binds to the promoter of the PTCRA gene, encoding pre-TCR-alpha. IK1-bindining sites overlap with RBPJ-binding sites. Therefore, IK1 competes with NOTCH3 coactivator complexes for binding to the PTCRA promoter (Bellavia et al. 2007). It is unknown which IK1 heterodimerization partners are involved in binding to the PTCRA promoter.
R-HSA-9017398 (Reactome) Based on the findings that the NOTCH3 coactivator complex component RBPJ (CSL) binds to RBPJ response elements in the promoter of the HEYL gene (Geimer Le Lay et al. 2014) and that HEYL gene expression is stimulated by NOTCH3 (Maier and Gessler 2000) and overlaps with NOTCH3 expression in vascular smooth muscle cells and the thymus (Leimeister et al. 2000), it can be concluded that the NOTCH3 coactivator complex binds the promoter of the HEYL gene.
R-HSA-9017797 (Reactome) YBX1 (YB-1) binds to EGF repeats in the extracellular domain of NOTCH3 (Rauen et al. 2009). While YBX1 is extensively studied as a protein involved in mRNA processing, it is also secreted by mesangial cells and monocytes during inflammation and can act as an extracellular mitogen (Frye et al. 2009).
R-HSA-9017817 (Reactome) Binding of NOTCH3 to YBX1 (YB-1) triggers cleavage of NOTCH3 by the gamma-secretase complex. The exact steps of YBX1-mediated NOTCH3 activation are not known. The gamma-secretase complex releases NOTCH3 intracellular domain (NICD3), leading to activation of NOTCH3 target genes downstream of YBX1 (Rauen et al. 2009).
R-HSA-9017855 (Reactome) Based on studies in mice, the intracellular domain of NOTCH3, NICD3, binds to transforming acidic coiled-coil protein-3 (TACC3). The interaction involves the ankyrin repeats of NOTCH3. The two proteins co-localize in the cytosol and possibly in the nucleus. TACC3 is implicated as a negative regulator of NOTCH signaling and may compete with NOTCH binding to RPBJ (Bargo et al. 2010).
R-HSA-9018538 (Reactome) Binding of the NOTCH3 coactivator complex to RBPJ response elements in the promoter of the DLGAP5 gene stimulates DLGAP5 transcription. DLGAP5 is involved in G2/M transition and is overexpressed in ovarian cancer cells. NOTCH3 gene is frequently amplified in ovarian cancer (Chen et al. 2012).
R-HSA-9018542 (Reactome) NOTCH3 coactivator complex binds the RBPJ (CSL) response elements in the promoter of the DLGAP5 gene. Two adjacent CSL-binding motifs in the DLGAP5 promoter, which are 6 bp apart, are both necessary for NOTCH3-mediated induction of DLGAP5 transcription (Chen et al. 2012).
R-HSA-9018572 (Reactome) EGFR phosphorylates intracellular domain of NOTCH3 (NICD3) on an unknown tyrosine residue. EGFR signaling inhibits NICD3-mediated transcription. It is not known whether EGFR-mediated phosphorylation of NICD3 affects NICD3 nuclear translocation or the formation of the NOTCH3 coactivator complex. Erlotinib treatment, which inhibits EGFR activation, results in increased NOTCH3 signaling and induction of stem-like phenotype in treated cells (Arasada et al. 2014).
R-HSA-9018573 (Reactome) The intracellular domain of NOTCH3 (NICD3) co-immunoprecipitates with ligand activated, autophosphorylated EGFR. Binding of NOTCH3 to EGFR is inhibited by erlotinib treatment, which prevents EGFR activation (Arasada et al. 2014).
R-HSA-9021325 (Reactome) NOTCH3 coactivator complex, composed of at least NICD3 (NOTCH3 intracellular domain), RBPJ and MAML (MAML1, MAML2 or MAML3; possibly MAMLD1), directly binds to RBPJ elements in the HES1 gene promoter (Lin et al. 2002). STAT1 can bind to STAT response elements in the HES1 gene promoter and enhance HES1 transcription induced by NICD3. In breast cancer, stromal cells secrete RNA-containing exosomes. Upon uptake of exosomes by breast cancer cells, STAT1-dependent antiviral signaling is initiated. Stromal cells also express JAG1 ligand on their surface and, in parallel to STAT1 signaling, activate NOTCH3 signaling in NOTCH3-expressing breast cancer cells. Synergistic activation of NOTCH3 and STAT1 increases breast cancer radiation resistance (Boelens et al. 2014).
R-HSA-9021334 (Reactome) STAT1 can bind to STAT response elements in the HEY1 gene promoter and enhances HEY1 transcription induced by NICD3 (Boelens et al. 2014).
R-HSA-9021406 (Reactome) NOTCH3 coactivator complex binds to RBPJ-binding element in the promoter region of the PBX1 gene (Park et al. 2008).
R-HSA-9021412 (Reactome) PBX1 gene transcription is directly stimulated by the NOTCH3 coactivator complex (Park et al. 2008). NOTCH3 gene is amplified in high-grade ovarian serous carcinoma and NOTCH3 overexpression contributes to cancer cell growth (Park et al. 2006). Overexpression of NOTCH3 in high-grade ovarian serous carcinoma correlates with overexpression of PBX1 (Park et al. 2006, Park et al. 2008). PBX1 gene encodes a homeodomain transcription factor PBX1 which is a B cell leukemia (B-ALL) oncogene (Nourse et al. 1990, Kamps et al. 1991) and is also overexpressed in melanoma (Shiraishi et al. 2007).
R-HSA-9021450 (Reactome) PLXND1 gene transcription is stimulated by NOTCH1 and NOTCH3 coactivator complexes. PLXND1 encodes the semaphorin receptor Plexin-D1, involved in neuronal migration as well as cancer cell invasiveness (Rehman et al. 2016).
R-HSA-9021451 (Reactome) PLXND1 gene promoter possesses an RBPJ-binding site which is necessary for NOTCH1 and NOTCH3-mediated induction of PLXND1 transcription. It is therefore concluded that NOTCH1 and NOTCH3 co-activator complexes directly bind to the PLXND1 gene promoter (Rehman et al. 2016).
R-HSA-9021478 (Reactome) The NOTCH3 coactivator complex directly stimulates WWC1 gene transcription. WWC1 gene encodes the protein Kibra, involved in Hippo signaling. NOTCH3-mediated induction of WWC1 positively regulates Hippo signaling and inhibits epithelial-to-mesenchymal transition (EMT) in triple negative breast cancer cells (Zhang et al. 2016).
R-HSA-9021480 (Reactome) The WWC1 (Kibra) gene promoter region contains two RBPJ-binding sites, one on the sense strand and one on the antisense strand. The NOTCH3 coactivator complex binds to the RBPJ-binding site on the antisense strand (Zhang et al. 2016).
R-HSA-9021520 (Reactome) WWP2, an E3 ubiquitin ligase, can interact with NOTCH3 cleavage products NEXT3 and NICD3 in the cytosol. The interaction involves the PPPY motif in the PEST domain of NOTCH3 (Jung et al. 2014).
R-HSA-9021523 (Reactome) WWP2, an E3 ubiquitin ligase, ubiquitinates NOTCH3 cleavage fragments NEXT3 and NICD3 in the cytosol, targeting them for lysosome-mediated degradation. WWP2 thus negatively regulates NOTCH3 signaling. WWP2 is a putative tumor suppressor whose deletions have been reported in the majority of ovarian carcinomas (Jung et al. 2014).
RBPJR-HSA-9013631 (Reactome)
TACC3R-HSA-9017855 (Reactome)
Ub-DLL/JAG:NOTCH3(40-1628)ArrowR-HSA-9013284 (Reactome)
Ub-DLL/JAG:NOTCH3ArrowR-HSA-9013069 (Reactome)
Ub-DLL/JAG:NOTCH3R-HSA-9013284 (Reactome)
Ub-NEXT3,Ub-NICD3:WWP2ArrowR-HSA-9021523 (Reactome)
UbR-HSA-9013069 (Reactome)
UbR-HSA-9021523 (Reactome)
WWC1 geneR-HSA-9021478 (Reactome)
WWC1 geneR-HSA-9021480 (Reactome)
WWC1ArrowR-HSA-9021478 (Reactome)
WWP2R-HSA-9021520 (Reactome)
YBX1:NOTCH3ArrowR-HSA-9017797 (Reactome)
YBX1:NOTCH3R-HSA-9017817 (Reactome)
YBX1R-HSA-9017797 (Reactome)
gamma-secretase complexmim-catalysisR-HSA-9013361 (Reactome)
gamma-secretase complexmim-catalysisR-HSA-9017817 (Reactome)
p-Y-NICD3ArrowR-HSA-9018572 (Reactome)
p-Y701-STAT1 dimer:HEY1 geneArrowR-HSA-9014129 (Reactome)
p-Y701-STAT1 dimer:HEY1 geneArrowR-HSA-9021334 (Reactome)
p-Y701-STAT1 dimerR-HSA-9021325 (Reactome)
p-Y701-STAT1 dimerR-HSA-9021334 (Reactome)

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