Regulation of DNA replication (Homo sapiens)

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152159, 10162, 8, 11, 141615135, 7171, 171518cytosolnucleoplasmPSMA5 ORC4 ORC2 ORC6 p-CDC6CDT1 GMNN MCM10 CDK2 CDT1 ORC4 UBC(229-304) ORC5 RPS27A(1-76) PSMD6 UBC(77-152) MCM7 MCM7 PSME4 PSMD13 UBA52(1-76) PSMD2 ubiquitinated Cdc6UBC(229-304) ORC5 Mcm4,6,7 complexPSMB2 UBC(1-76) UBC(457-532) UBB(77-152) UBB(153-228) UBC(77-152) MCM2ATPPSMA7 MCM4 MCM5 UBC(77-152) MCM3MCM6 MCM6 UBC(77-152) MCM7 SHFM1 p-T160-CDK2 UBC(153-228) RPS27A(1-76) MCM2 PSMD14 PSMA4 origin of replication UBC(533-608) ORC1 UBA52(1-76) UBB(153-228) MCM3 UBC(1-76) UBC(229-304) UBC(229-304) UBB(77-152) origin of replication UBB(1-76) UBC(153-228) UBC(153-228) UBC(609-684) MCM10:activepre-replicativecomplexCdt1:gemininORC6 UBB(153-228) ADPUBB(1-76) UBC(457-532) UBB(77-152) CDT1UBC(1-76) UBC(457-532) RPS27A(1-76) CCNA2 GMNNPSME2 UBB(77-152) ORC4 UBC(533-608) CDKN1B UBC(1-76) UBC(609-684) ORC5 MCM4 UBC(457-532) CyclinA:Cdk2:substratecomplexPSMC4 UBC(1-76) CDC6 cyclin UBB(153-228) UBC(153-228) E2F1 ORC5 PSMD9 UBC(153-228) UBB(77-152) ORC2 UBC(381-456) MCM6 CCNA1 Orc4:Orc5:Orc3:Orc2:originCDT1 UBC(533-608) UBC(1-76) ORC3 UBC(381-456) UBC(229-304) RB1 MCM3 CDC6 PSMC6 PSMD4 MCM4 MCM6 p-CDC6UBB(153-228) ORC1 FZR1 E2F1/2/3PSMD10 MCM4 UBC(77-152) PSMC5 UBB(1-76) PSMC1 PSMD11 ADPORC6 Orc1:Orc4:Orc5:Orc3:Orc2:originUbPSMF1 PSMB7 UBC(457-532) UBA52(1-76) anaphase-promotingcomplex (APC)E2F3 ORC4 MCM4 MCM8 UBB(1-76) MCM2 p-S,T-ORC1PSMB6 PSMC2 PSME1 MCM3 UBC(305-380) PSMD1 CDT1PSMB3 MCM7 PSME3 ORC4 origin of replication RPS27A(1-76) 26S proteasomePSMA3 CDC6 E2F2 origin of replication UBC(457-532) ORC2 PSMB5 UBC(305-380) ORC3 MCM10 ORC1 MCM3 ORC4 PSMA8 UBC(533-608) UBC(381-456) RPS27A(1-76) MCM2-7MCM6 PSMD3 UBA52(1-76) ORC5 ATPgeminin:ubiquitincomplexUBC(381-456) MCM6 MCM5 UbATPUBC(305-380) PSMD7 Cdt1:gemininCDKPSMC3 PSMA1 MCM2 UBC(305-380) MCM5 ubiquitinated Orc1UBC(305-380) ORC1 PSMB9 RPS27A(1-76) PSMD8 MCM3 MCM5MCM5 ORC5 GMNN PSMA2 UBC(381-456) UBC(533-608) ORC1 CDT1 MCM8 PSMB1 UBC(533-608) MCM10:pre-replicative complexUBC(305-380) UBA52(1-76) MCM7 UBC(77-152) UBC(153-228) CDT1 PSMD5 PSMB11 PSMA6 ORC3 UBB(153-228) ORC2 UBB(1-76) CDKN1A PSMD12 UBB(1-76) PSMB10 origin of replication ORC1 UBA52(1-76) PSMB4 pre-replicativecomplex(Orc1-minus)UBC(609-684) ORC2 UBC(609-684) MCM4 ORC3 ORC6 GMNN UBC(609-684) CDC6 UBC(609-684) ORC1MCM2 PSMB8 MCM2 MCM5 UBC(229-304) CDC6MCM7 pre-replicativecomplexORC3 ORC2 MCM8 UBC(381-456) origin of replication MCM8 ubiquitinated Orc1ORC3 CDC6 UBB(77-152) 1816152, 11, 14154, 633, 122, 11, 14


Description

DNA replication is regulated at various levels via ORC proteins. This pathway includes annotation of individual events that lead to the regulation of replication. View original pathway at:Reactome.

Comments

Reactome-Converter 
Pathway is converted from Reactome ID: 69304
Reactome-version 
Reactome version: 63

Quality Tags

Ontology Terms

 

Bibliography

View all...
  1. McGarry TJ, Kirschner MW.; ''Geminin, an inhibitor of DNA replication, is degraded during mitosis.''; PubMed Europe PMC
  2. Méndez J, Stillman B.; ''Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis.''; PubMed Europe PMC
  3. Wei SJ, Williams JG, Dang H, Darden TA, Betz BL, Humble MM, Chang FM, Trempus CS, Johnson K, Cannon RE, Tennant RW.; ''Identification of a specific motif of the DSS1 protein required for proteasome interaction and p53 protein degradation.''; PubMed Europe PMC
  4. Harper JW, Burton JL, Solomon MJ.; ''The anaphase-promoting complex: it's not just for mitosis any more.''; PubMed Europe PMC
  5. Schaarschmidt D, Ladenburger EM, Keller C, Knippers R.; ''Human Mcm proteins at a replication origin during the G1 to S phase transition.''; PubMed Europe PMC
  6. Zachariae W, Nasmyth K.; ''Whose end is destruction: cell division and the anaphase-promoting complex.''; PubMed Europe PMC
  7. Sato M, Gotow T, You Z, Komamura-Kohno Y, Uchiyama Y, Yabuta N, Nojima H, Ishimi Y.; ''Electron microscopic observation and single-stranded DNA binding activity of the Mcm4,6,7 complex.''; PubMed Europe PMC
  8. Li CJ, DePamphilis ML.; ''Mammalian Orc1 protein is selectively released from chromatin and ubiquitinated during the S-to-M transition in the cell division cycle.''; PubMed Europe PMC
  9. Ohtani K, Tsujimoto A, Ikeda M, Nakamura M.; ''Regulation of cell growth-dependent expression of mammalian CDC6 gene by the cell cycle transcription factor E2F.''; PubMed Europe PMC
  10. Yan Z, DeGregori J, Shohet R, Leone G, Stillman B, Nevins JR, Williams RS.; ''Cdc6 is regulated by E2F and is essential for DNA replication in mammalian cells.''; PubMed Europe PMC
  11. Dhar SK, Delmolino L, Dutta A.; ''Architecture of the human origin recognition complex.''; PubMed Europe PMC
  12. Voges D, Zwickl P, Baumeister W.; ''The 26S proteasome: a molecular machine designed for controlled proteolysis.''; PubMed Europe PMC
  13. Bell SP, Dutta A.; ''DNA replication in eukaryotic cells.''; PubMed Europe PMC
  14. Vashee S, Simancek P, Challberg MD, Kelly TJ.; ''Assembly of the human origin recognition complex.''; PubMed Europe PMC
  15. Méndez J, Zou-Yang XH, Kim SY, Hidaka M, Tansey WP, Stillman B.; ''Human origin recognition complex large subunit is degraded by ubiquitin-mediated proteolysis after initiation of DNA replication.''; PubMed Europe PMC
  16. Jiang W, Wells NJ, Hunter T.; ''Multistep regulation of DNA replication by Cdk phosphorylation of HsCdc6.''; PubMed Europe PMC
  17. Wohlschlegel JA, Dwyer BT, Dhar SK, Cvetic C, Walter JC, Dutta A.; ''Inhibition of eukaryotic DNA replication by geminin binding to Cdt1.''; PubMed Europe PMC
  18. Petersen BO, Wagener C, Marinoni F, Kramer ER, Melixetian M, Lazzerini Denchi E, Gieffers C, Matteucci C, Peters JM, Helin K.; ''Cell cycle- and cell growth-regulated proteolysis of mammalian CDC6 is dependent on APC-CDH1.''; PubMed Europe PMC

History

View all...
CompareRevisionActionTimeUserComment
99909view16:06, 31 October 2018ReactomeTeamreactome version 63
99465view14:39, 31 October 2018ReactomeTeamreactome version 62 (2nd attempt)
93850view13:40, 16 August 2017ReactomeTeamreactome version 61
93408view11:22, 9 August 2017ReactomeTeamreactome version 61
86497view09:19, 11 July 2016ReactomeTeamreactome version 56
83249view10:30, 18 November 2015ReactomeTeamVersion54
81355view12:52, 21 August 2015ReactomeTeamVersion53
76824view08:04, 17 July 2014ReactomeTeamFixed remaining interactions
76528view11:50, 16 July 2014ReactomeTeamFixed remaining interactions
75861view09:51, 11 June 2014ReactomeTeamRe-fixing comment source
75561view10:36, 10 June 2014ReactomeTeamReactome 48 Update
74916view13:44, 8 May 2014AnweshaFixing comment source for displaying WikiPathways description
74560view08:36, 30 April 2014ReactomeTeamReactome46
45052view19:13, 6 October 2011ThomasOntology Term : 'DNA replication pathway' added !
42114view21:58, 4 March 2011MaintBotAutomatic update
39924view05:56, 21 January 2011MaintBotNew pathway

External references

DataNodes

View all...
NameTypeDatabase referenceComment
26S proteasomeComplexR-HSA-68819 (Reactome)
ADPMetaboliteCHEBI:16761 (ChEBI)
ATPMetaboliteCHEBI:15422 (ChEBI)
CCNA1 ProteinP78396 (Uniprot-TrEMBL)
CCNA2 ProteinP20248 (Uniprot-TrEMBL)
CDC6 ProteinQ99741 (Uniprot-TrEMBL)
CDC6ProteinQ99741 (Uniprot-TrEMBL)
CDK2 ProteinP24941 (Uniprot-TrEMBL)
CDKN1A ProteinP38936 (Uniprot-TrEMBL)
CDKN1B ProteinP46527 (Uniprot-TrEMBL)
CDKComplexR-HSA-68380 (Reactome)
CDT1 ProteinQ9H211 (Uniprot-TrEMBL)
CDT1ProteinQ9H211 (Uniprot-TrEMBL)
Cdt1:gemininComplexR-HSA-156502 (Reactome)
Cdt1:gemininComplexR-HSA-68537 (Reactome)
Cyclin

A:Cdk2:substrate

complex
ComplexR-HSA-187947 (Reactome)
E2F1 ProteinQ01094 (Uniprot-TrEMBL)
E2F1/2/3ComplexR-HSA-68640 (Reactome)
E2F2 ProteinQ14209 (Uniprot-TrEMBL)
E2F3 ProteinO00716 (Uniprot-TrEMBL)
FZR1 ProteinQ9UM11 (Uniprot-TrEMBL)
GMNN ProteinO75496 (Uniprot-TrEMBL)
GMNNProteinO75496 (Uniprot-TrEMBL)
MCM10 ProteinQ7L590 (Uniprot-TrEMBL)
MCM10:active

pre-replicative

complex
ComplexR-HSA-156564 (Reactome)
MCM10:pre-replicative complexComplexR-HSA-68560 (Reactome)
MCM2 ProteinP49736 (Uniprot-TrEMBL)
MCM2-7ComplexR-HSA-68558 (Reactome)
MCM2ProteinP49736 (Uniprot-TrEMBL)
MCM3 ProteinP25205 (Uniprot-TrEMBL)
MCM3ProteinP25205 (Uniprot-TrEMBL)
MCM4 ProteinP33991 (Uniprot-TrEMBL)
MCM5 ProteinP33992 (Uniprot-TrEMBL)
MCM5ProteinP33992 (Uniprot-TrEMBL)
MCM6 ProteinQ14566 (Uniprot-TrEMBL)
MCM7 ProteinP33993 (Uniprot-TrEMBL)
MCM8 ProteinQ9UJA3 (Uniprot-TrEMBL)
Mcm4,6,7 complexComplexR-HSA-69018 (Reactome)
ORC1 ProteinQ13415 (Uniprot-TrEMBL)
ORC1ProteinQ13415 (Uniprot-TrEMBL)
ORC2 ProteinQ13416 (Uniprot-TrEMBL)
ORC3 ProteinQ9UBD5 (Uniprot-TrEMBL)
ORC4 ProteinO43929 (Uniprot-TrEMBL)
ORC5 ProteinO43913 (Uniprot-TrEMBL)
ORC6 ProteinQ9Y5N6 (Uniprot-TrEMBL)
Orc1:Orc4:Orc5:Orc3:Orc2:originComplexR-HSA-68592 (Reactome)
Orc4:Orc5:Orc3:Orc2:originComplexR-HSA-68520 (Reactome)
PSMA1 ProteinP25786 (Uniprot-TrEMBL)
PSMA2 ProteinP25787 (Uniprot-TrEMBL)
PSMA3 ProteinP25788 (Uniprot-TrEMBL)
PSMA4 ProteinP25789 (Uniprot-TrEMBL)
PSMA5 ProteinP28066 (Uniprot-TrEMBL)
PSMA6 ProteinP60900 (Uniprot-TrEMBL)
PSMA7 ProteinO14818 (Uniprot-TrEMBL)
PSMA8 ProteinQ8TAA3 (Uniprot-TrEMBL)
PSMB1 ProteinP20618 (Uniprot-TrEMBL)
PSMB10 ProteinP40306 (Uniprot-TrEMBL)
PSMB11 ProteinA5LHX3 (Uniprot-TrEMBL)
PSMB2 ProteinP49721 (Uniprot-TrEMBL)
PSMB3 ProteinP49720 (Uniprot-TrEMBL)
PSMB4 ProteinP28070 (Uniprot-TrEMBL)
PSMB5 ProteinP28074 (Uniprot-TrEMBL)
PSMB6 ProteinP28072 (Uniprot-TrEMBL)
PSMB7 ProteinQ99436 (Uniprot-TrEMBL)
PSMB8 ProteinP28062 (Uniprot-TrEMBL)
PSMB9 ProteinP28065 (Uniprot-TrEMBL)
PSMC1 ProteinP62191 (Uniprot-TrEMBL)
PSMC2 ProteinP35998 (Uniprot-TrEMBL)
PSMC3 ProteinP17980 (Uniprot-TrEMBL)
PSMC4 ProteinP43686 (Uniprot-TrEMBL)
PSMC5 ProteinP62195 (Uniprot-TrEMBL)
PSMC6 ProteinP62333 (Uniprot-TrEMBL)
PSMD1 ProteinQ99460 (Uniprot-TrEMBL)
PSMD10 ProteinO75832 (Uniprot-TrEMBL)
PSMD11 ProteinO00231 (Uniprot-TrEMBL)
PSMD12 ProteinO00232 (Uniprot-TrEMBL)
PSMD13 ProteinQ9UNM6 (Uniprot-TrEMBL)
PSMD14 ProteinO00487 (Uniprot-TrEMBL)
PSMD2 ProteinQ13200 (Uniprot-TrEMBL)
PSMD3 ProteinO43242 (Uniprot-TrEMBL)
PSMD4 ProteinP55036 (Uniprot-TrEMBL)
PSMD5 ProteinQ16401 (Uniprot-TrEMBL)
PSMD6 ProteinQ15008 (Uniprot-TrEMBL)
PSMD7 ProteinP51665 (Uniprot-TrEMBL)
PSMD8 ProteinP48556 (Uniprot-TrEMBL)
PSMD9 ProteinO00233 (Uniprot-TrEMBL)
PSME1 ProteinQ06323 (Uniprot-TrEMBL)
PSME2 ProteinQ9UL46 (Uniprot-TrEMBL)
PSME3 ProteinP61289 (Uniprot-TrEMBL)
PSME4 ProteinQ14997 (Uniprot-TrEMBL)
PSMF1 ProteinQ92530 (Uniprot-TrEMBL)
RB1 ProteinP06400 (Uniprot-TrEMBL)
RPS27A(1-76) ProteinP62979 (Uniprot-TrEMBL)
SHFM1 ProteinP60896 (Uniprot-TrEMBL)
UBA52(1-76) ProteinP62987 (Uniprot-TrEMBL)
UBB(1-76) ProteinP0CG47 (Uniprot-TrEMBL)
UBB(153-228) ProteinP0CG47 (Uniprot-TrEMBL)
UBB(77-152) ProteinP0CG47 (Uniprot-TrEMBL)
UBC(1-76) ProteinP0CG48 (Uniprot-TrEMBL)
UBC(153-228) ProteinP0CG48 (Uniprot-TrEMBL)
UBC(229-304) ProteinP0CG48 (Uniprot-TrEMBL)
UBC(305-380) ProteinP0CG48 (Uniprot-TrEMBL)
UBC(381-456) ProteinP0CG48 (Uniprot-TrEMBL)
UBC(457-532) ProteinP0CG48 (Uniprot-TrEMBL)
UBC(533-608) ProteinP0CG48 (Uniprot-TrEMBL)
UBC(609-684) ProteinP0CG48 (Uniprot-TrEMBL)
UBC(77-152) ProteinP0CG48 (Uniprot-TrEMBL)
UbComplexR-HSA-113595 (Reactome)
UbComplexR-HSA-68524 (Reactome)
anaphase-promoting complex (APC)R-HSA-69007 (Reactome)
cyclin R-HSA-68379 (Reactome)
geminin:ubiquitin complexComplexR-HSA-68585 (Reactome)
origin of replication R-ALL-68419 (Reactome)
p-CDC6ProteinQ99741 (Uniprot-TrEMBL)
p-S,T-ORC1ProteinQ13415 (Uniprot-TrEMBL)
p-T160-CDK2 ProteinP24941 (Uniprot-TrEMBL)
pre-replicative

complex

(Orc1-minus)
ComplexR-HSA-157563 (Reactome)
pre-replicative complexComplexR-HSA-68559 (Reactome)
ubiquitinated Cdc6ComplexR-HSA-68570 (Reactome)
ubiquitinated Orc1ComplexR-HSA-113570 (Reactome)
ubiquitinated Orc1ComplexR-HSA-68586 (Reactome)

Annotated Interactions

View all...
SourceTargetTypeDatabase referenceComment
26S proteasomemim-catalysisR-HSA-68948 (Reactome)
26S proteasomemim-catalysisR-HSA-69016 (Reactome)
ADPArrowR-HSA-68944 (Reactome)
ADPArrowR-HSA-69005 (Reactome)
ATPR-HSA-68944 (Reactome)
ATPR-HSA-69005 (Reactome)
ATPR-HSA-69015 (Reactome)
CDC6ArrowR-HSA-68637 (Reactome)
CDC6R-HSA-69005 (Reactome)
CDKmim-catalysisR-HSA-69005 (Reactome)
CDT1ArrowR-HSA-68712 (Reactome)
CDT1ArrowR-HSA-68940 (Reactome)
CDT1R-HSA-69299 (Reactome)
Cdt1:gemininArrowR-HSA-69299 (Reactome)
Cdt1:gemininR-HSA-68712 (Reactome)
Cyclin

A:Cdk2:substrate

complex
mim-catalysisR-HSA-68944 (Reactome)
E2F1/2/3ArrowR-HSA-68637 (Reactome)
GMNNR-HSA-69299 (Reactome)
MCM10:active

pre-replicative

complex
ArrowR-HSA-68940 (Reactome)
MCM10:pre-replicative complexR-HSA-68940 (Reactome)
MCM2-7R-HSA-69019 (Reactome)
MCM2ArrowR-HSA-69019 (Reactome)
MCM3ArrowR-HSA-69019 (Reactome)
MCM5ArrowR-HSA-69019 (Reactome)
Mcm4,6,7 complexArrowR-HSA-69019 (Reactome)
ORC1R-HSA-68611 (Reactome)
Orc1:Orc4:Orc5:Orc3:Orc2:originArrowR-HSA-68611 (Reactome)
Orc4:Orc5:Orc3:Orc2:originR-HSA-68611 (Reactome)
R-HSA-68611 (Reactome) At the beginning of this reaction, 1 molecule of 'Orc4:Orc5:Orc3:Orc2:origin', and 1 molecule of 'Orc1' are present. At the end of this reaction, 1 molecule of 'Orc1:Orc4:Orc5:Orc3:Orc2:origin' is present.

This reaction takes place in the 'nucleus'.

R-HSA-68637 (Reactome) At the end of this reaction, 1 molecule of 'Cdc6' is present.



R-HSA-68712 (Reactome) At the beginning of this reaction, 1 molecule of 'ubiquitin', and 1 molecule of 'Cdt1:geminin' are present. At the end of this reaction, 1 molecule of 'geminin:ubiquitin complex', and 1 molecule of 'Cdt1' are present.

This reaction takes place in the 'cytosol'.

R-HSA-68940 (Reactome) At the beginning of this reaction, 1 molecule of 'Mcm10:pre-replicative complex' is present. At the end of this reaction, 1 molecule of 'Mcm10:active pre-replicative complex', and 1 molecule of 'CDT1' are present.

This reaction takes place in the 'nucleus'.

R-HSA-68944 (Reactome) At the beginning of this reaction, 1 molecule of 'ATP', and 1 molecule of 'pre-replicative complex' are present. At the end of this reaction, 1 molecule of 'phosphorylated Orc1', 1 molecule of 'pre-replicative complex (Orc1-minus)', and 1 molecule of 'ADP' are present.

This reaction takes place in the 'nucleus' and is mediated by the 'kinase activity' of 'Cyclin A:Cdk2 complex'.

R-HSA-68946 (Reactome) At the beginning of this reaction, 1 molecule of 'ubiquitin', and 1 molecule of 'phosphorylated Orc1' are present. At the end of this reaction, 1 molecule of 'ubiquitinated Orc1' is present.

This reaction takes place in the 'nucleus'.

R-HSA-68947 (Reactome) In this reaction, 1 molecule of 'ubiquitinated Orc1' is translocated from nucleoplasm to cytosol.

This movement of the molecule occurs through the 'nuclear pore'.

R-HSA-68948 (Reactome) At the beginning of this reaction, 1 molecule of 'ubiquitinated Orc1' is present. At the end of this reaction, 1 molecule of 'ubiquitin' is present.

This reaction takes place in the 'cytosol' and is mediated by the 'endopeptidase activity' of '26S proteasome'.

R-HSA-69005 (Reactome) At the beginning of this reaction, 1 molecule of 'CDC6', and 1 molecule of 'ATP' are present. At the end of this reaction, 1 molecule of 'ADP', and 1 molecule of 'phosphorylated Cdc6' are present.

This reaction takes place in the 'nucleus' and is mediated by the 'kinase activity' of 'CDK'.

R-HSA-69006 (Reactome) In this reaction, 1 molecule of 'phosphorylated Cdc6' is translocated from nucleoplasm to cytosol.

This movement of the molecule occurs through the 'nuclear pore'.

R-HSA-69015 (Reactome) At the beginning of this reaction, 1 molecule of 'phosphorylated Cdc6', 1 molecule of 'ubiquitin', and 1 molecule of 'ATP' are present. At the end of this reaction, 1 molecule of 'ubiquitinated Cdc6' is present.

This reaction takes place in the 'cytosol' and is mediated by the 'endopeptidase activity' of 'anaphase-promoting complex (APC)'.

R-HSA-69016 (Reactome) At the beginning of this reaction, 1 molecule of 'ubiquitinated Cdc6' is present. At the end of this reaction, 1 molecule of 'ubiquitin' is present.

This reaction takes place in the 'cytosol' and is mediated by the 'endopeptidase activity' of '26S proteasome'.

R-HSA-69019 (Reactome) At the start of the elongation phase of DNA replication, the Mcm2-7 complex may re-arrange to function as the replicative helicase associated with the replication fork. In general, a replicative helicase is associated with the replication fork and unwinds DNA ahead of the polymerase. In yeast, the Mcm proteins associate with origin DNA in G1 phase and then exit the origin upon replication initiation, consistent with moving out of the origin with the replication fork. The Mcm2-7 complex is a ring-shaped hexamer. Complexes of Mcm4, Mcm6 and Mcm7 proteins from humans or S. pombe display a modest ATP-dependent helicase activity in vitro. Consistent with the hypothesis that eukaryotic Mcm complexes function as helicases, an archaeal Mcm homolog is a ring-shaped double hexamer that has a processive DNA unwinding activity. Mcm proteins may have additional functions during elongation, as uninterrupted function of all six is required for replication fork progression in budding yeast. Mcm4,6,7 helicase activity may be negatively regulated in two ways. Mcm2, Mcm4, Mcm6, and Mcm7 also form a stable complex which, however, has no helicase activity, suggesting that Mcm2 inhibits DNA unwinding by Mcm4,6,7. In addition, phosphorylation of human Mcm4,6,7 complex by CDK inhibits its helicase activity.
R-HSA-69299 (Reactome) At the beginning of this reaction, 1 molecule of 'geminin', and 1 molecule of 'CDT1' are present. At the end of this reaction, 1 molecule of 'Cdt1:geminin' is present.

This reaction takes place in the 'nucleoplasm'.

UbArrowR-HSA-68948 (Reactome)
UbArrowR-HSA-69016 (Reactome)
UbR-HSA-68712 (Reactome)
UbR-HSA-68946 (Reactome)
UbR-HSA-69015 (Reactome)
anaphase-promoting complex (APC)mim-catalysisR-HSA-69015 (Reactome)
geminin:ubiquitin complexArrowR-HSA-68712 (Reactome)
p-CDC6ArrowR-HSA-69005 (Reactome)
p-CDC6ArrowR-HSA-69006 (Reactome)
p-CDC6R-HSA-69006 (Reactome)
p-CDC6R-HSA-69015 (Reactome)
p-S,T-ORC1ArrowR-HSA-68944 (Reactome)
p-S,T-ORC1R-HSA-68946 (Reactome)
pre-replicative

complex

(Orc1-minus)
ArrowR-HSA-68944 (Reactome)
pre-replicative complexR-HSA-68944 (Reactome)
ubiquitinated Cdc6ArrowR-HSA-69015 (Reactome)
ubiquitinated Cdc6R-HSA-69016 (Reactome)
ubiquitinated Orc1ArrowR-HSA-68946 (Reactome)
ubiquitinated Orc1ArrowR-HSA-68947 (Reactome)
ubiquitinated Orc1R-HSA-68947 (Reactome)
ubiquitinated Orc1R-HSA-68948 (Reactome)
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