Eukaryotic Translation Termination (Homo sapiens)

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1, 573, 824, 67cytosolRPL10L RPL39L RPL35 RPS8 RPL22L1 RPS13 RPS4Y1 RPS24 18S rRNA RPL37A RPS24 RPL17 GSPT1 RPS15A RPS29 GTP RPL8 RPL10 RPL15 RPS14 RPL40 RPL39 RPL11 RPL27 RPS4Y1 RPS25 RPL36AL RPL3 RPLP2 RPL10A 5S rRNA RPL12 ETF1 dimer:eRF3:GTPRPLP2 GSPT2 RPS28 RPL3 RPL32 RPL21 RPL5 TRMT112 RPS3 GSPT2 RPL10A RPL5 RPS26 RPS24 RPL23 RPL14 RPL10L RPL9 RPS3A FAU RPL37 RPL22 PiRPS19 RPL39 RPS27L RPS3A RPLP0 RPL22L1 RPS21 RPL6 GSPT1 RPL5 RPL18A RPS15 RPL6 RPL34 RPL26L1 RPL17 RPL22 RPL10 RPS19 RPS4X RPS26 RPL36AL RPL40 RPL28 RPS16 RPLP1 RPL3 RPL34 RPL35 Ceruloplasmin mRNA RPS23 RPS28 RPL31 GTP RPS3 RPL29 5S rRNA RPL13 RPL35A RPL18A Ceruloplasmin mRNA RPL18 RPL30 RPS23 RPL17 RPL27 peptidyl-tRNA with elongated peptide RPL7 RPL7A RPL18 RPSA RPL14 RPL5 GSPT1 RPS23 RPL37 protein RPS12 peptidyl-tRNA with elongated peptide RPS27L RPL32 APEHRPS11 RPL7 RPL19 RPS27L Me-Q185-ETF1dimer:eRF3:GDP:80SRibosome:mRNA:tRNAComplexRPS25 RPS9 RPS12 peptidyl-tRNA with elongated peptide RPL9 RPL30 RPL13 N6AMT1:TRMT112GTP RPS8 RPL30 RPSA 28S rRNA RPL4 RPL14 RPS27 RPL7 RPS4X FAU RPL18 RPL6 RPL35 RPL10L RPL24 18S rRNA RPS21 RPS18 RPL8 RPL7A RPL19 RPLP1 Me-Q185-ETF1dimer:eRF3:GTPRPS17 RPS11 RPS15A RPL27 RPL37A AdoHcyRPL26 RPL37A RPL35A RPL27A RPS4Y1 RPS14 RPS6 RPL39L RPS2 RPLP1 NAc-Ser residue RPL32 RPL21 Me-Q185-ETF1 RPS10 RPL37A RPS27 RPL39L RPL4 18S rRNA RPL36A RPS21 RPL39L 5.8S rRNA RPL3L RPS19 RPS7 Me-Q185-ETF1dimer:eRF3:GDP:80SRibosome:mRNA:peptidyl-tRNA ComplexRPL22 RPL18A RPL36 RPL35A RPS17 RPL9 GSPT2 RPL23A RPS17 RPS27L RPL23A RPL27A RPL19 RPL22L1 RPS16 RPL4 RPS7 RPS4X RPL41 RPL10 RPS17 RPS2 RPS27A(77-156) RPS6 RPSA RPS15 RPS5 RPS10 RPL27 RPL30 RPL15 RPS4Y2 RPL40 RPL26L1 Ceruloplasmin mRNA RPL19 RPS20 RPS7 RPS5 RPS11 RPL15 RPL38 RPS12 RPS28 Ceruloplasmin mRNA NAc-Ser residueRPS15 RPL11 RPS20 RPS5 RPL8 RPL38 H2ORPL36A RPS11 GDP RPS14 RPS27 RPL13 RPL36 RPL34 RPS27 RPL29 RPL17 RPL37 5.8S rRNA Me-Q185-ETF1 RPL29 RPS4Y2 RPS20 RPL23A RPS18 RPL3L RPLP2 RPL41 N6AMT1 RPL28 RPL18A RPL12 RPS27A(77-156) RPLP2 RPL24 Me-Q185-ETF1dimer:eRF3:GTP:80SRibosome:mRNA:peptidyl-tRNA28S rRNA RPL22 RPL11 FAU RPS2 RPS27A(77-156) RPL35A RPS13 RPL8 RPS25 RPS3 RPL36AL RPL23 RPL9 5.8S rRNA RPS6 RPL34 RPS10 RPL7A RPL36AL RPL26 RPL7A RPS4Y2 RPS29 RPL23 RPL13A RPL7 RPL28 RPS29 Me-Q185-ETF1 RPS28 RPL39 RPL23 RPS16 RPL36 RPS21 RPS9 28S rRNA RPL27A RPL6 RPS9 proteinRPL3L ETF1 Me-Q185-ETF1 5S rRNA RPL28 RPS5 RPL14 RPS3A FAU RPL13A 18S rRNA RPL15 RPL13 RPL4 80SRibosome:mRNA:peptidyl-tRNA with elongating peptideRPL26L1 RPL36A RPS26 RPS13 RPL32 RPS4Y1 RPL38 GSPT2 RPL13A 5S rRNA RPS15A RPS12 RPS9 5.8S rRNA RPSA 28S rRNA RPS29 RPL10A RPS26 RPL31 RPL36A RPL26 RPL38 RPS4X RPS6 NAc-Ser:proteinRPS2 RPL10 RPLP1 RPL12 RPS15A RPS13 GSPT2 RPLP0 RPL22L1 RPS8 RPS4Y2 RPL3 RPL35 RPL27A RPL39 H2ORPS10 RPS14 RPL11 RPS24 RPS18 RPL18 RPL13A tRNA GDP RPLP0 RPL23A RPL36 AdoMetRPS19 RPS8 RPS7 RPS23 RPS3A RPS27A(77-156) RPL24 GSPT1 RPLP0 RPL10A RPL3L RPL41 RPS20 RPL31 RPL10L RPL31 RPL12 GSPT1 RPS25 RPL29 RPL26 RPL37 RPL24 RPL21 RPL21 RPS3 RPS15 RPL26L1 RPS16 RPL41 RPL40 RPS18


The arrival of any of the three stop codons (UAA, UAG and UGA) into the ribosomal A-site triggers the binding of a release factor (RF) to the ribosome and subsequent polypeptide chain release. In eukaryotes, the RF is composed of two proteins, eRF1 and eRF3. eRF1 is responsible for the hydrolysis of the peptidyl-tRNA, while eRF3 provides a GTP-dependent function. The ribosome releases the mRNA and dissociates into its two complex subunits, which can reassemble on another molecule to begin a new round of protein synthesis. It should be noted that at present, there is no factor identified in eukaryotes that would be the functional equivalent of the bacterial ribosome release (or recycling) factor, RRF, that catalyzes dissociation of the ribosome from the mRNA following release of the polypeptide View original pathway at:Reactome.


Pathway is converted from Reactome ID: 72764
Reactome version: 66
Reactome Author 
Reactome Author: Bedwell, David M

Quality Tags

Ontology Terms



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  1. Ichikawa S, Kaji A.; ''Molecular cloning and expression of ribosome releasing factor.''; PubMed Europe PMC
  2. Figaro S, Scrima N, Buckingham RH, Heurgué-Hamard V.; ''HemK2 protein, encoded on human chromosome 21, methylates translation termination factor eRF1.''; PubMed Europe PMC
  3. Hoshino S, Imai M, Mizutani M, Kikuchi Y, Hanaoka F, Ui M, Katada T.; ''Molecular cloning of a novel member of the eukaryotic polypeptide chain-releasing factors (eRF). Its identification as eRF3 interacting with eRF1.''; PubMed Europe PMC
  4. Erlandsson R, Boldog F, Persson B, Zabarovsky ER, Allikmets RL, Sümegi J, Klein G, Jörnvall H.; ''The gene from the short arm of chromosome 3, at D3F15S2, frequently deleted in renal cell carcinoma, encodes acylpeptide hydrolase.''; PubMed Europe PMC
  5. Salas-Marco J, Bedwell DM.; ''GTP hydrolysis by eRF3 facilitates stop codon decoding during eukaryotic translation termination.''; PubMed Europe PMC
  6. Jones WM, Scaloni A, Bossa F, Popowicz AM, Schneewind O, Manning JM.; ''Genetic relationship between acylpeptide hydrolase and acylase, two hydrolytic enzymes with similar binding but different catalytic specificities.''; PubMed Europe PMC
  7. Hoshino S, Imai M, Kobayashi T, Uchida N, Katada T.; ''The eukaryotic polypeptide chain releasing factor (eRF3/GSPT) carrying the translation termination signal to the 3'-Poly(A) tail of mRNA. Direct association of erf3/GSPT with polyadenylate-binding protein.''; PubMed Europe PMC
  8. Frolova L, Le Goff X, Rasmussen HH, Cheperegin S, Drugeon G, Kress M, Arman I, Haenni AL, Celis JE, Philippe M.; ''A highly conserved eukaryotic protein family possessing properties of polypeptide chain release factor.''; PubMed Europe PMC


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101594view11:46, 1 November 2018ReactomeTeamreactome version 66
101130view21:31, 31 October 2018ReactomeTeamreactome version 65
100658view20:05, 31 October 2018ReactomeTeamreactome version 64
100208view16:50, 31 October 2018ReactomeTeamreactome version 63
99759view15:16, 31 October 2018ReactomeTeamreactome version 62 (2nd attempt)
93873view13:42, 16 August 2017ReactomeTeamreactome version 61
93440view11:23, 9 August 2017ReactomeTeamreactome version 61
86531view09:20, 11 July 2016ReactomeTeamreactome version 56
83397view11:07, 18 November 2015ReactomeTeamVersion54
81591view13:08, 21 August 2015ReactomeTeamVersion53
77051view08:35, 17 July 2014ReactomeTeamFixed remaining interactions
76756view12:11, 16 July 2014ReactomeTeamFixed remaining interactions
76081view10:14, 11 June 2014ReactomeTeamRe-fixing comment source
75791view11:32, 10 June 2014ReactomeTeamReactome 48 Update
75141view14:08, 8 May 2014AnweshaFixing comment source for displaying WikiPathways description
74832view10:05, 30 April 2014ReactomeTeamReactome46
74788view08:52, 30 April 2014ReactomeTeamReactome46
68957view17:38, 8 July 2013MaintBotUpdated to 2013 gpml schema
45251view18:37, 7 October 2011AlexanderPicoOntology Term : 'translation termination pathway' added !
42036view21:52, 4 March 2011MaintBotAutomatic update
39839view05:52, 21 January 2011MaintBotNew pathway

External references


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NameTypeDatabase referenceComment
18S rRNA ProteinX03205 (EMBL)
28S rRNA ProteinM11167 (EMBL)
5.8S rRNA ProteinJ01866 (EMBL)
5S rRNA ProteinV00589 (EMBL)
80S Ribosome:mRNA:peptidyl-tRNA with elongating peptideComplexR-HSA-141952 (Reactome)
APEHProteinP13798 (Uniprot-TrEMBL)
AdoHcyMetaboliteCHEBI:16680 (ChEBI)
AdoMetMetaboliteCHEBI:15414 (ChEBI)
Ceruloplasmin mRNA ProteinM13699 (EMBL)
ETF1 ProteinP62495 (Uniprot-TrEMBL)
ETF1 dimer:eRF3:GTPComplexR-HSA-6800132 (Reactome)
FAU ProteinP62861 (Uniprot-TrEMBL)
GDP MetaboliteCHEBI:17552 (ChEBI)
GSPT1 ProteinP15170 (Uniprot-TrEMBL)
GSPT2 ProteinQ8IYD1 (Uniprot-TrEMBL)
GTP MetaboliteCHEBI:15996 (ChEBI)
H2OMetaboliteCHEBI:15377 (ChEBI)


Ribosome:mRNA:peptidyl-tRNA Complex
ComplexR-HSA-143381 (Reactome)

dimer:eRF3:GDP:80S Ribosome:mRNA:tRNA

ComplexR-HSA-143396 (Reactome)


ComplexR-HSA-143397 (Reactome)
Me-Q185-ETF1 dimer:eRF3:GTPComplexR-HSA-6800154 (Reactome)
Me-Q185-ETF1 ProteinP62495 (Uniprot-TrEMBL)
N6AMT1 ProteinQ9Y5N5 (Uniprot-TrEMBL)
N6AMT1:TRMT112ComplexR-HSA-6800133 (Reactome)
NAc-Ser residue MetaboliteCHEBI:83690 (ChEBI)
NAc-Ser residueMetaboliteCHEBI:83690 (ChEBI)
NAc-Ser:proteinComplexR-ALL-5691496 (Reactome)
PiMetaboliteCHEBI:18367 (ChEBI)
RPL10 ProteinP27635 (Uniprot-TrEMBL)
RPL10A ProteinP62906 (Uniprot-TrEMBL)
RPL10L ProteinQ96L21 (Uniprot-TrEMBL)
RPL11 ProteinP62913 (Uniprot-TrEMBL)
RPL12 ProteinP30050 (Uniprot-TrEMBL)
RPL13 ProteinP26373 (Uniprot-TrEMBL)
RPL13A ProteinP40429 (Uniprot-TrEMBL)
RPL14 ProteinP50914 (Uniprot-TrEMBL)
RPL15 ProteinP61313 (Uniprot-TrEMBL)
RPL17 ProteinP18621 (Uniprot-TrEMBL)
RPL18 ProteinQ07020 (Uniprot-TrEMBL)
RPL18A ProteinQ02543 (Uniprot-TrEMBL)
RPL19 ProteinP84098 (Uniprot-TrEMBL)
RPL21 ProteinP46778 (Uniprot-TrEMBL)
RPL22 ProteinP35268 (Uniprot-TrEMBL)
RPL22L1 ProteinQ6P5R6 (Uniprot-TrEMBL)
RPL23 ProteinP62829 (Uniprot-TrEMBL)
RPL23A ProteinP62750 (Uniprot-TrEMBL)
RPL24 ProteinP83731 (Uniprot-TrEMBL)
RPL26 ProteinP61254 (Uniprot-TrEMBL)
RPL26L1 ProteinQ9UNX3 (Uniprot-TrEMBL)
RPL27 ProteinP61353 (Uniprot-TrEMBL)
RPL27A ProteinP46776 (Uniprot-TrEMBL)
RPL28 ProteinP46779 (Uniprot-TrEMBL)
RPL29 ProteinP47914 (Uniprot-TrEMBL)
RPL3 ProteinP39023 (Uniprot-TrEMBL)
RPL30 ProteinP62888 (Uniprot-TrEMBL)
RPL31 ProteinP62899 (Uniprot-TrEMBL)
RPL32 ProteinP62910 (Uniprot-TrEMBL)
RPL34 ProteinP49207 (Uniprot-TrEMBL)
RPL35 ProteinP42766 (Uniprot-TrEMBL)
RPL35A ProteinP18077 (Uniprot-TrEMBL)
RPL36 ProteinQ9Y3U8 (Uniprot-TrEMBL)
RPL36A ProteinP83881 (Uniprot-TrEMBL)
RPL36AL ProteinQ969Q0 (Uniprot-TrEMBL)
RPL37 ProteinP61927 (Uniprot-TrEMBL)
RPL37A ProteinP61513 (Uniprot-TrEMBL)
RPL38 ProteinP63173 (Uniprot-TrEMBL)
RPL39 ProteinP62891 (Uniprot-TrEMBL)
RPL39L ProteinQ96EH5 (Uniprot-TrEMBL)
RPL3L ProteinQ92901 (Uniprot-TrEMBL)
RPL4 ProteinP36578 (Uniprot-TrEMBL)
RPL40 ProteinP62987 (Uniprot-TrEMBL)
RPL41 ProteinP62945 (Uniprot-TrEMBL)
RPL5 ProteinP46777 (Uniprot-TrEMBL)
RPL6 ProteinQ02878 (Uniprot-TrEMBL)
RPL7 ProteinP18124 (Uniprot-TrEMBL)
RPL7A ProteinP62424 (Uniprot-TrEMBL)
RPL8 ProteinP62917 (Uniprot-TrEMBL)
RPL9 ProteinP32969 (Uniprot-TrEMBL)
RPLP0 ProteinP05388 (Uniprot-TrEMBL)
RPLP1 ProteinP05386 (Uniprot-TrEMBL)
RPLP2 ProteinP05387 (Uniprot-TrEMBL)
RPS10 ProteinP46783 (Uniprot-TrEMBL)
RPS11 ProteinP62280 (Uniprot-TrEMBL)
RPS12 ProteinP25398 (Uniprot-TrEMBL)
RPS13 ProteinP62277 (Uniprot-TrEMBL)
RPS14 ProteinP62263 (Uniprot-TrEMBL)
RPS15 ProteinP62841 (Uniprot-TrEMBL)
RPS15A ProteinP62244 (Uniprot-TrEMBL)
RPS16 ProteinP62249 (Uniprot-TrEMBL)
RPS17 ProteinP08708 (Uniprot-TrEMBL)
RPS18 ProteinP62269 (Uniprot-TrEMBL)
RPS19 ProteinP39019 (Uniprot-TrEMBL)
RPS2 ProteinP15880 (Uniprot-TrEMBL)
RPS20 ProteinP60866 (Uniprot-TrEMBL)
RPS21 ProteinP63220 (Uniprot-TrEMBL)
RPS23 ProteinP62266 (Uniprot-TrEMBL)
RPS24 ProteinP62847 (Uniprot-TrEMBL)
RPS25 ProteinP62851 (Uniprot-TrEMBL)
RPS26 ProteinP62854 (Uniprot-TrEMBL)
RPS27 ProteinP42677 (Uniprot-TrEMBL)
RPS27A(77-156) ProteinP62979 (Uniprot-TrEMBL)
RPS27L ProteinQ71UM5 (Uniprot-TrEMBL)
RPS28 ProteinP62857 (Uniprot-TrEMBL)
RPS29 ProteinP62273 (Uniprot-TrEMBL)
RPS3 ProteinP23396 (Uniprot-TrEMBL)
RPS3A ProteinP61247 (Uniprot-TrEMBL)
RPS4X ProteinP62701 (Uniprot-TrEMBL)
RPS4Y1 ProteinP22090 (Uniprot-TrEMBL)
RPS4Y2 ProteinQ8TD47 (Uniprot-TrEMBL)
RPS5 ProteinP46782 (Uniprot-TrEMBL)
RPS6 ProteinP62753 (Uniprot-TrEMBL)
RPS7 ProteinP62081 (Uniprot-TrEMBL)
RPS8 ProteinP62241 (Uniprot-TrEMBL)
RPS9 ProteinP46781 (Uniprot-TrEMBL)
RPSA ProteinP08865 (Uniprot-TrEMBL)
TRMT112 ProteinQ9UI30 (Uniprot-TrEMBL)
peptidyl-tRNA with elongated peptide R-ALL-141678 (Reactome)
protein MetaboliteCHEBI:36080 (ChEBI)
proteinMetaboliteCHEBI:36080 (ChEBI)
tRNA R-HSA-141679 (Reactome)

Annotated Interactions

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SourceTargetTypeDatabase referenceComment
80S Ribosome:mRNA:peptidyl-tRNA with elongating peptideR-HSA-141691 (Reactome)
APEHmim-catalysisR-HSA-5691512 (Reactome)
AdoHcyArrowR-HSA-6800138 (Reactome)
AdoMetR-HSA-6800138 (Reactome)
ETF1 dimer:eRF3:GTPR-HSA-6800138 (Reactome)
H2OR-HSA-141673 (Reactome)
H2OR-HSA-5691512 (Reactome)


Ribosome:mRNA:peptidyl-tRNA Complex
ArrowR-HSA-141673 (Reactome)


Ribosome:mRNA:peptidyl-tRNA Complex
R-HSA-141671 (Reactome)

dimer:eRF3:GDP:80S Ribosome:mRNA:tRNA

ArrowR-HSA-141671 (Reactome)


ArrowR-HSA-141691 (Reactome)


R-HSA-141673 (Reactome)
Me-Q185-ETF1 dimer:eRF3:GTPArrowR-HSA-6800138 (Reactome)
Me-Q185-ETF1 dimer:eRF3:GTPR-HSA-141691 (Reactome)
N6AMT1:TRMT112mim-catalysisR-HSA-6800138 (Reactome)
NAc-Ser residueArrowR-HSA-5691512 (Reactome)
NAc-Ser:proteinArrowR-HSA-141671 (Reactome)
NAc-Ser:proteinR-HSA-5691512 (Reactome)
PiArrowR-HSA-141673 (Reactome)
R-HSA-141671 (Reactome) Please note that this reaction was inferred from experiments performed using Saccharomyces cerevisiae.
R-HSA-141673 (Reactome) Please note that this reaction was inferred from experiments performed using Saccharomyces cerevisiae.
R-HSA-141691 (Reactome) Please note that this reaction was inferred from experiments performed using Saccharomyces cerevisiae.
R-HSA-5691512 (Reactome) Protein acetylation, which can occur during or after polypeptide chain biosynthesis, helps protect the intracellular proteins from proteolysis. Acylamino-acid-releasing enzyme (APEH) is a cytosolic enzyme able to catalyse the preferential hydrolysis of terminal acetylated amino acids from small acetylated peptides. APEH prefers substrates with acetylated methionine, alanine and serine residues. Hydrolysis produces an acetylated amino acid and a N-terminus protein (Jones et al. 1991). APEH expression is reduced in renal cell carcinoma therefore may represent a tumor suppressor gene, whose loss contributes to the development of renal cell carcinoma (Erlandsson et al. 1991). The hydrolysis of an acetylated serine residue (NAc-Ser-protein) is shown here.
R-HSA-6800138 (Reactome) Class 1 release factors such as eukaryotic peptide chain release factor subunit 1 (ETF1) direct the termination of peptide translation in response to the termination codons UAA, UAG and UGA. ETF1 needs to be complexed with ERF3 in its GTP-bound form to be efficiently post-translationally methylated. HemK methyltransferase family member 2 (N6AMT1) is a heterodimeric methyltransferase that catalyses N5-methylation of ETF1 on glutamine 185 (Q185), using S-adenosyl L-methionine (AdoMet) as the methyl donor (Figaro et al. 2008). N6AMT1 forms a complex with multifunctional methyltransferase subunit TRM112-like protein (TRMT112)
proteinArrowR-HSA-5691512 (Reactome)
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