Eukaryotic transcription initiation (WP948)

Pan troglodytes

In eukaryotes, RNA polymerase, and therefore the initiation of transcription, requires the presence of a core promoter sequence in the DNA. RNA polymerase is able to bind to core promoters in the presence of various specific transcription factors. The most common type of core promoter in eukaryotes is a short DNA sequence known as a TATA box. The TATA box, as a core promoter, is the binding site for a transcription factor known as TATA binding protein (TBP), which is itself a subunit of another transcription factor, called Transcription Factor II D (TFIID). After TFIID binds to the TATA box via the TBP, five more transcription factors and RNA polymerase combine around the TATA box in a series of stages to form a preinitiation complex. One transcription factor, DNA helicase, has helicase activity and so is involved in the separating of opposing strands of double-stranded DNA to provide access to a single-stranded DNA template. However, only a low, or basal, rate of transcription is driven by the preinitiation complex alone. Other proteins known as activators and repressors, along with any associated coactivators or corepressors, are responsible for modulating transcription rate. Source: [[wikipedia:Transcription_(genetics)|Wikipedia]]
last edited


Thomas Kelder , Christine Chichester , and Eric Weitz

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Pathway Ontology: transcription pathway


Label Type Compact Identifier Comment
TBP GeneProduct ncbigene:738472
GTF2B GeneProduct ncbigene:743356
TAF5 GeneProduct ncbigene:735428
TAF6 GeneProduct ncbigene:463588
TAF7 GeneProduct ncbigene:471669
LOC100612178 GeneProduct ncbigene:100612178
ILK GeneProduct ncbigene:748941
TAF12 GeneProduct ncbigene:456686
TAF13 GeneProduct ncbigene:738503
GTF2A2 GeneProduct ncbigene:453483
GTF2F2 GeneProduct ncbigene:741868
GTF2E2 GeneProduct ncbigene:464098
CDK7 GeneProduct ncbigene:462378
GTF2H1 GeneProduct ncbigene:748842
GTF2H2 GeneProduct ncbigene:461830
GTF2H4 GeneProduct ncbigene:462549
ERCC3 GeneProduct ncbigene:470526
ERCC2 GeneProduct ncbigene:456124
POLR1B GeneProduct ncbigene:459495
POLR3B GeneProduct ncbigene:452201
POLR2A GeneProduct ncbigene:454463
POLR2F GeneProduct ncbigene:458824
POLR2C GeneProduct ncbigene:454119
POLR2E GeneProduct ncbigene:456359
POLR2K GeneProduct ncbigene:741257
POLR3D GeneProduct ncbigene:464043
POLR3E GeneProduct ncbigene:454351
POLR3H GeneProduct ncbigene:745514
POLR3K GeneProduct ncbigene:744386
POLR1A GeneProduct ncbigene:459373
POLR2I GeneProduct ncbigene:746261
POLR2G GeneProduct ncbigene:740537
POLR2H GeneProduct ncbigene:471027
POLR2B GeneProduct ncbigene:461305
CCNH GeneProduct ncbigene:739351
MNAT1 GeneProduct ncbigene:452954
GTF2H3 GeneProduct ncbigene:452352
POLR1E GeneProduct ncbigene:747551


  1. Regulation of gene expression by TBP-associated proteins. Lee TI, Young RA. Genes Dev. 1998 May 15;12(10):1398–408. PubMed Europe PMC Scholia
  2. Orchestrated response: a symphony of transcription factors for gene control. Lemon B, Tjian R. Genes Dev. 2000 Oct 15;14(20):2551–69. PubMed Europe PMC Scholia