Effects of PIP2 hydrolysis (Homo sapiens)

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Curation Tags

Description

Hydrolysis of phosphatidyl inositol-bisphosphate (PIP2) by phospholipase C (PLC) produces diacylglycerol (DAG) and inositol triphosphate (IP3). Both are potent second messengers. IP3 diffuses into the cytosol, but as DAG is a hydrophobic lipid it remains within the plasma membrane. IP3 stimulates the release of calcium ions from the smooth endoplasmic reticulum, while DAG activates the conventional and unconventional protein kinase C (PKC) isoforms, facilitating the translocation of PKC from the cytosol to the plasma membrane. The effects of DAG are mimicked by tumor-promoting phorbol esters. DAG is also a precursor for the biosynthesis of prostaglandins, the endocannabinoid 2-arachidonoylglycerol and an activator of a subfamily of TRP-C (Transient Receptor Potential Canonical) cation channels 3, 6, and 7.Original Pathway at Reactome: http://www.reactome.org/PathwayBrowser/#DB=gk_current&FOCUS_SPECIES_ID=48887&FOCUS_PATHWAY_ID=114508

Ontology Tags

 

Bibliography

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History

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CompareRevisionActionTimeUserComment
76979view08:26, 17 July 2014ReactomeTeamFixed remaining interactions
76684view12:05, 16 July 2014ReactomeTeamFixed remaining interactions
76011view10:07, 11 June 2014ReactomeTeamRe-fixing comment source
75719view11:08, 10 June 2014ReactomeTeamReactome 48 Update
75071view13:58, 8 May 2014AnweshaFixing comment source for displaying WikiPathways description
74716view08:47, 30 April 2014ReactomeTeamReactome46
42032view21:51, 4 March 2011MaintBotAutomatic update
39835view05:52, 21 January 2011MaintBotNew pathway

External references

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NameTypeDatabase referenceComment
1,2-diacyl-glycerol 3-phosphateCHEBI:29089 (ChEBI)
ITPR2 [platelet

dense tubular

network membrane]
ProteinQ14571 (Uniprot-TrEMBL)
2AGMetaboliteCHEBI:52392 (ChEBI)
ITPR3 [platelet

dense tubular

network membrane]
ProteinQ14573 (Uniprot-TrEMBL)
IP3 receptorComplexREACT_18961 (Reactome)
ITPR1 [platelet

dense tubular

network membrane]
ProteinQ14643 (Uniprot-TrEMBL)
AAMetaboliteCHEBI:15843 (ChEBI)
I(1,4,5)P3MetaboliteCHEBI:16595 (ChEBI)
RASGRP1 [cytosol]ProteinO95267 (Uniprot-TrEMBL)
IP3 receptor bound to IP3 and Ca++ComplexREACT_4346 (Reactome)
TRPC7 [plasma membrane]ProteinQ9HCX4 (Uniprot-TrEMBL)
I(1,4,5)P3 [platelet

dense tubular

network membrane]
MetaboliteCHEBI:16595 (ChEBI)
Diacylglycerol lipaseProteinREACT_19676 (Reactome)
Ca2+ [platelet dense

tubular network

membrane]
MetaboliteCHEBI:29108 (ChEBI)
ATPMetaboliteCHEBI:15422 (ChEBI)
Ca2+MetaboliteCHEBI:29108 (ChEBI)
CalDAG-GEFs:DAG:Ca2+ComplexREACT_24405 (Reactome)
CalDAG-GEFsProteinREACT_18194 (Reactome) Rap1 can be activated by certain GEFs that respond to calcium and diacylglycerol (CalDAG-GEFs).
H2OMetaboliteCHEBI:15377 (ChEBI)
DAGCHEBI:17815 (ChEBI)
TRPC3(1-848) [plasma membrane]ProteinQ13507 (Uniprot-TrEMBL)
Protein kinase C, novel isoformsProteinREACT_22540 (Reactome) These isoforms of PKC bind DAG and phosphatidylserine (PS), but unlike the conventional forms, do not require calcium for PS binding.
TRPC6 [plasma membrane]ProteinQ9Y210 (Uniprot-TrEMBL)
Protein kinase C, novel isoforms:DAGComplexREACT_23271 (Reactome)
Fatty AcidCHEBI:35366 (ChEBI)
DAG [plasma membrane]MetaboliteCHEBI:17815 (ChEBI)
GlycerolMetaboliteCHEBI:17754 (ChEBI)
PRKCD(1-676) [cytosol]ProteinQ05655 (Uniprot-TrEMBL)
MGLLProteinQ99685 (Uniprot-TrEMBL)
PRKCE [cytosol]ProteinQ02156 (Uniprot-TrEMBL)
ADPMetaboliteCHEBI:16761 (ChEBI)
PRKCH [cytosol]ProteinP24723 (Uniprot-TrEMBL)
Diacylglycerol kinaseProteinREACT_20449 (Reactome)
PRKCQ [cytosol]ProteinQ04759 (Uniprot-TrEMBL)
RASGRP2 [cytosol]ProteinQ7LDG7 (Uniprot-TrEMBL)
TRPC3/6/7ProteinREACT_23108 (Reactome)
Ca2+ [cytosol]MetaboliteCHEBI:29108 (ChEBI)
DAG-activated TRPC3/6/7ComplexREACT_24061 (Reactome)
H+MetaboliteCHEBI:15378 (ChEBI)
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